Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_M02
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 315 4e-87
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 311 1e-85
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 1e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 3e-32
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 29 0.63
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 28 1.5
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 315 bits (774), Expect = 4e-87
Identities = 138/203 (67%), Positives = 167/203 (82%)
Frame = +3
Query: 45 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 224
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 225 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 404
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 405 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 584
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 585 VVEPYNSILTTHTTLEHSDCAFM 653
VVEPYNS+LTTH TL+ +DC FM
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFM 203
Score = 81.4 bits (192), Expect = 1e-16
Identities = 36/45 (80%), Positives = 42/45 (93%)
Frame = +1
Query: 655 VDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGAL 789
VDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASL+F+G+L
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSL 248
Score = 33.5 bits (73), Expect = 0.039
Identities = 14/18 (77%), Positives = 14/18 (77%)
Frame = +2
Query: 794 VDLTXFQTNLVPYPRYXF 847
VDL FQTNLVPYPR F
Sbjct: 250 VDLAEFQTNLVPYPRIHF 267
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 311 bits (763), Expect = 1e-85
Identities = 140/208 (67%), Positives = 170/208 (81%), Gaps = 5/208 (2%)
Frame = +3
Query: 45 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 209
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 210 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 389
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 390 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 569
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 570 QVSTAVVEPYNSILTTHTTLEHSDCAFM 653
QVST+VVEPYNS+LTTH TL++SDC FM
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Score = 82.6 bits (195), Expect = 6e-17
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +1
Query: 655 VDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGAL 789
VDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASL+F G+L
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252
Score = 33.5 bits (73), Expect = 0.039
Identities = 14/18 (77%), Positives = 14/18 (77%)
Frame = +2
Query: 794 VDLTXFQTNLVPYPRYXF 847
VDL FQTNLVPYPR F
Sbjct: 254 VDLNEFQTNLVPYPRIHF 271
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 161 bits (391), Expect = 1e-40
Identities = 79/202 (39%), Positives = 113/202 (55%)
Frame = +3
Query: 45 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 224
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 225 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 404
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 405 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 584
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 585 VVEPYNSILTTHTTLEHSDCAF 650
VVEPYN+ L+ H +E+SD F
Sbjct: 179 VVEPYNATLSMHQLVENSDETF 200
Score = 42.7 bits (96), Expect = 6e-05
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +1
Query: 655 VDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGAL 789
+DNEA+ I L I+ P+Y +LN L+ +++ +T S +F G L
Sbjct: 202 IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGEL 246
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (321), Expect = 3e-32
Identities = 72/191 (37%), Positives = 110/191 (57%), Gaps = 3/191 (1%)
Frame = +3
Query: 48 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 227
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 228 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 401
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 402 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 578
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 579 TAVVEPYNSIL 611
VV+PYNS+L
Sbjct: 180 DVVVQPYNSLL 190
Score = 32.7 bits (71), Expect = 0.068
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +1
Query: 655 VDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDG 783
+DN A+ I L + PT+ N+L+ ++S+ T +L++ G
Sbjct: 205 LDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 29.5 bits (63), Expect = 0.63
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = -3
Query: 771 QRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 667
Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 184 QKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -3
Query: 786 GAVEFQRSSDRGDNLSDETIQVGVGWALNVEITAAD 679
G ++FQ D G +SD++I +G+ + V + A +
Sbjct: 308 GNIQFQEGQDGGSVISDKSITEFLGYLIGVPVAAIE 343
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 124 STASSLMARCPQTRPSGVETILSTLSSARPELAST 228
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 143 WPDAHRQDHRGWRRFFQHFLQR 208
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,382,374
Number of Sequences: 5004
Number of extensions: 69538
Number of successful extensions: 224
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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