Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_L23
(900 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 246 4e-66
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 235 5e-63
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 123 4e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 106 5e-24
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 246 bits (601), Expect = 4e-66
Identities = 109/177 (61%), Positives = 134/177 (75%)
Frame = +3
Query: 123 MRECISVHVGQAGVXIGNACWELYCLEHGIQPDGXMPTDXTIGGGDDSFNTFFSXTGAGK 302
MRE IS+HVGQAG IGNACWELYCLEHGIQP+G M + D F+TFFS TG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 303 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVDLVLD 482
+VPR+++VDLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 483 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLXMERLSX*LRQEV*XEFAIYPAPQV 653
+IR++AD C+GLQGFL+FH L +ERL+ ++ +F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177
Score = 38.7 bits (86), Expect = 0.001
Identities = 18/41 (43%), Positives = 21/41 (51%)
Frame = +2
Query: 638 PRASGXTAVVXPYNSILTTHXXLXTLTVLSWXDNXXXYEIC 760
P T+VV PYNS+LTTH L DN Y+IC
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 235 bits (576), Expect = 5e-63
Identities = 107/181 (59%), Positives = 134/181 (74%), Gaps = 4/181 (2%)
Frame = +3
Query: 123 MRECISVHVGQAGVXIGNACWELYCLEHGIQPDGXMPTDXTIGGGD----DSFNTFFSXT 290
MRE ISVHVGQAGV IGNACWELYCLEHGI PDG + + + D F TFFS T
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 291 GAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVD 470
G GK VPR+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 471 LVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLXMERLSX*LRQEV*XEFAIYPAPQ 650
VL+RIR++AD C+GLQGFL+FH L +ERL+ ++ +F++YPAPQ
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 651 V 653
V
Sbjct: 181 V 181
Score = 38.7 bits (86), Expect = 0.001
Identities = 18/41 (43%), Positives = 21/41 (51%)
Frame = +2
Query: 638 PRASGXTAVVXPYNSILTTHXXLXTLTVLSWXDNXXXYEIC 760
P T+VV PYNS+LTTH L DN Y+IC
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 123 bits (296), Expect = 4e-29
Identities = 62/176 (35%), Positives = 90/176 (51%)
Frame = +3
Query: 123 MRECISVHVGQAGVXIGNACWELYCLEHGIQPDGXMPTDXTIGGGDDSFNTFFSXTGAGK 302
MRE + + GQ G +G A W EHG+ G T + N +F+ GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 303 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVDLVLD 482
+VPRAV VDLEP +D V++G + LF P +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 483 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLXMERLSX*LRQEV*XEFAIYPAPQ 650
+R+ A+ C LQGF + H L + ++ + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 106 bits (254), Expect = 5e-24
Identities = 57/177 (32%), Positives = 95/177 (53%), Gaps = 2/177 (1%)
Frame = +3
Query: 126 RECISVHVGQAGVXIGNACWELYCLEHGIQPDGXMPTDXTIGGGDDSFNTFFSXTGAGKH 305
RE I++ GQ G IG+ W+ CLEHGI PDG + + T G D + FF + ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 306 VPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYTIGKEIVDLVL 479
+PRA+ +DLEP VV+ + + TY L++P ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 480 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLXMERLSX*LRQEV*XEFAIYPAPQ 650
D I + AD L+GF + H +ERL+ +++ ++++P Q
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.4
Identities = 21/74 (28%), Positives = 32/74 (43%)
Frame = +1
Query: 85 NSTFKHRKKLKSKCVSASLYTXAKPESXSVMPAGSFTAWSTASSLMAXCPQTXPSGVETI 264
+S+ + S + +S S S+ + T+ ST SSL + + PS T
Sbjct: 135 SSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTS 193
Query: 265 LSTLSSAXPELAST 306
ST SSA P S+
Sbjct: 194 SSTFSSAAPTSTSS 207
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,776,504
Number of Sequences: 5004
Number of extensions: 49560
Number of successful extensions: 124
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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