Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_L08
(849 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 313 2e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 305 6e-84
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 154 1e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 3e-32
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 2.5
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 26 5.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 313 bits (768), Expect = 2e-86
Identities = 155/245 (63%), Positives = 182/245 (74%), Gaps = 1/245 (0%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 646 VVEPYNSILTTPHNP*AL*LC-FHGXQ*SHL*HLPP*SRH*APTYTNLNRLIGQXVSSIT 822
VVEPYNS+LTT H L C F S P+Y NLNRLI Q VSSIT
Sbjct: 181 VVEPYNSVLTT-HATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239
Query: 823 ASLRF 837
ASLRF
Sbjct: 240 ASLRF 244
Score = 47.2 bits (107), Expect = 3e-06
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = +2
Query: 698 SDCAFMVXNEAIYDICRRNLDIER 769
+DC FMV NE+ YDICRRNLDIER
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIER 221
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 305 bits (748), Expect = 6e-84
Identities = 153/249 (61%), Positives = 181/249 (72%), Gaps = 5/249 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 QVSTAVVEPYNSILTTPHNP*AL*LCFHGXQ*SHL*HLPP*SRH*APTYTNLNRLIGQXV 810
QVST+VVEPYNS+LTT F + PTY NLNRLI Q V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 811 SSITASLRF 837
SSITASLRF
Sbjct: 240 SSITASLRF 248
Score = 51.2 bits (117), Expect = 2e-07
Identities = 23/29 (79%), Positives = 25/29 (86%)
Frame = +2
Query: 692 EHSDCAFMVXNEAIYDICRRNLDIERXPT 778
++SDC FMV NEA YDICRRNLDIER PT
Sbjct: 200 DNSDCTFMVDNEACYDICRRNLDIER-PT 227
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 154 bits (374), Expect = 1e-38
Identities = 86/247 (34%), Positives = 127/247 (51%), Gaps = 3/247 (1%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 646 VVEPYNSILT---TPHNP*AL*LCFHGXQ*SHL*HLPP*SRH*APTYTNLNRLIGQXVSS 816
VVEPYN+ L+ N C S + + +P+Y +LN L+ ++
Sbjct: 179 VVEPYNATLSMHQLVENSDET-FCIDNEALSSI--FANTLKIKSPSYDDLNHLVSAVMAG 235
Query: 817 ITASLRF 837
+T S RF
Sbjct: 236 VTTSFRF 242
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (321), Expect = 3e-32
Identities = 84/250 (33%), Positives = 134/250 (53%), Gaps = 7/250 (2%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 639
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 640 TAVVEPYNSILTTPH---NP*AL*LCFHGXQ*SHL*HLPP*SRH*A-PTYTNLNRLIGQX 807
VV+PYNS+L N ++ + + L H+ H PT+ N+L+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAA----LAHIAADRLHTQNPTFHQQNQLVSTV 235
Query: 808 VSSITASLRF 837
+S+ T +LR+
Sbjct: 236 MSASTTTLRY 245
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -2
Query: 365 NNCLYVPVRTPSTTVGSRSTKTARGTCLPAPVSLKKVLKESSPP 234
N+ +Y PV + STT S ST + T A S + +S P
Sbjct: 740 NDAVYYPVSSSSTTTTSTSTGSVTTTSTTATASCTLPISYTSTP 783
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/35 (45%), Positives = 24/35 (68%)
Frame = +3
Query: 693 STLTVLSWSTMKPSMTSAAVISTLSAXLHQPESSH 797
S +TV S ST+KPS+TS+ +ST S+ + SS+
Sbjct: 919 SNITVTS-STLKPSLTSS--VSTASSYIASSASSN 950
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,438,056
Number of Sequences: 5004
Number of extensions: 72051
Number of successful extensions: 205
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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