Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_J13
(808 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 357 e-100
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 352 3e-98
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 180 2e-46
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 147 2e-36
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.6
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 357 bits (879), Expect = e-100
Identities = 156/224 (69%), Positives = 188/224 (83%)
Frame = +1
Query: 40 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 219
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 220 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 399
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 400 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYSKKSKLEFAIYPAPQVSTA 579
+IR++AD C+GLQGFL+FH LL+ERL+++Y+KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 580 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTY 711
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224
Score = 43.6 bits (98), Expect = 3e-05
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = +3
Query: 702 PNLHNLNRLIGQIVSSITASLRFXGAL 782
P+ NLNRLI Q+VSSITASLRF G+L
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSL 248
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 352 bits (866), Expect = 3e-98
Identities = 159/229 (69%), Positives = 189/229 (82%), Gaps = 5/229 (2%)
Frame = +1
Query: 40 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 204
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 205 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 384
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 385 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYSKKSKLEFAIYPAP 564
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++Y KKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 565 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTY 711
QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
Score = 43.2 bits (97), Expect = 4e-05
Identities = 20/27 (74%), Positives = 22/27 (81%)
Frame = +3
Query: 702 PNLHNLNRLIGQIVSSITASLRFXGAL 782
P NLNRLI Q+VSSITASLRF G+L
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSL 252
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 180 bits (438), Expect = 2e-46
Identities = 88/224 (39%), Positives = 126/224 (56%)
Frame = +1
Query: 40 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 219
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 220 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 399
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 400 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYSKKSKLEFAIYPAPQVSTA 579
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 580 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTY 711
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 147 bits (356), Expect = 2e-36
Identities = 80/226 (35%), Positives = 125/226 (55%), Gaps = 3/226 (1%)
Frame = +1
Query: 43 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 222
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 223 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 396
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 397 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYSKKSKLEFAIYPAPQ-VS 573
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 574 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTY 711
VV+PYNS+L ++D ++DN A+ I L + PT+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.1 bits (57), Expect = 3.1
Identities = 22/74 (29%), Positives = 33/74 (44%)
Frame = +2
Query: 2 TGSLKHRKKLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETI 181
+ SL + S + +S S S+ + T+ ST SSL + ++PS T
Sbjct: 135 SSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTS 193
Query: 182 LSTLSSARPELAST 223
ST SSA P S+
Sbjct: 194 SSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 138 WPDAHRQDHRGWRRFFQHFLQR 203
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.6
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 29 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 151
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,240,887
Number of Sequences: 5004
Number of extensions: 66714
Number of successful extensions: 194
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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