Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP03_FL5_I24
         (901 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   272   4e-74
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   265   7e-72
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   136   5e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   118   9e-28
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.4  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.4  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  272 bits (667), Expect = 4e-74
 Identities = 119/177 (67%), Positives = 144/177 (81%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 631
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177



 Score = 79.8 bits (188), Expect = 5e-16
 Identities = 36/56 (64%), Positives = 41/56 (73%)
 Frame = +3

Query: 672 HTTLEHSDCAFMVDNEAIYDICRRXLDIERPXYXNXNRXIGXIVSSXTASLXXXGA 839
           H TL+ +DC FMVDNE+ YDICRR LDIERP Y N NR I  +VSS TASL   G+
Sbjct: 192 HATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGS 247



 Score = 28.7 bits (61), Expect = 1.2
 Identities = 12/19 (63%), Positives = 14/19 (73%)
 Frame = +1

Query: 616 PRASGXTAVVEPYNSILTT 672
           P     T+VVEPYNS+LTT
Sbjct: 173 PAPQVSTSVVEPYNSVLTT 191


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  265 bits (649), Expect = 7e-72
 Identities = 120/182 (65%), Positives = 146/182 (80%), Gaps = 5/182 (2%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 625
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 626 QV 631
           QV
Sbjct: 180 QV 181



 Score = 83.8 bits (198), Expect = 3e-17
 Identities = 38/56 (67%), Positives = 42/56 (75%)
 Frame = +3

Query: 672 HTTLEHSDCAFMVDNEAIYDICRRXLDIERPXYXNXNRXIGXIVSSXTASLXXXGA 839
           H TL++SDC FMVDNEA YDICRR LDIERP Y N NR I  +VSS TASL   G+
Sbjct: 196 HATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGS 251



 Score = 28.7 bits (61), Expect = 1.2
 Identities = 12/19 (63%), Positives = 14/19 (73%)
 Frame = +1

Query: 616 PRASGXTAVVEPYNSILTT 672
           P     T+VVEPYNS+LTT
Sbjct: 177 PAPQVSTSVVEPYNSVLTT 195


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  136 bits (328), Expect = 5e-33
 Identities = 66/176 (37%), Positives = 96/176 (54%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 628
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174



 Score = 40.3 bits (90), Expect = 4e-04
 Identities = 18/55 (32%), Positives = 28/55 (50%)
 Frame = +3

Query: 672 HTTLEHSDCAFMVDNEAIYDICRRXLDIERPXYXNXNRXIGXIVSSXTASLXXXG 836
           H  +E+SD  F +DNEA+  I    L I+ P Y + N  +  +++  T S    G
Sbjct: 190 HQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  118 bits (285), Expect = 9e-28
 Identities = 63/177 (35%), Positives = 99/177 (55%), Gaps = 2/177 (1%)
 Frame = +2

Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 628
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 180 STASSLMARCPQTRPSGVETILSTLSSARPELAST 284
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,396,798
Number of Sequences: 5004
Number of extensions: 68648
Number of successful extensions: 232
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)