Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_H22
(899 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 200 2e-52
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch... 29 0.90
SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces pomb... 28 2.1
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 2.7
SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 27 3.6
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.8
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 27 4.8
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha... 27 4.8
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 26 6.3
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 8.4
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 8.4
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom... 26 8.4
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 200 bits (488), Expect = 2e-52
Identities = 102/199 (51%), Positives = 127/199 (63%), Gaps = 1/199 (0%)
Frame = +2
Query: 227 RLSGIICTIGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKL 406
R + IICTIGP S NV L K+ + GMN+ RMNFSHGS+EYH I N R+A +
Sbjct: 26 RRTSIICTIGPKSNNVETLCKLRDAGMNIVRMNFSHGSYEYHQSVIDNARKASAT----- 80
Query: 407 GSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYK 586
F LAIALDTKGPEIRTGL GG + + G + TT Y EK N +Y+DYK
Sbjct: 81 NPLFPLAIALDTKGPEIRTGLTVGG--TDYPISSGHEMIFTTDDAYAEKCNDKVMYIDYK 138
Query: 587 NITNVVKPGNRIFIDDGLISI-ICQSVSADTLTCTIENGGMLGSRKGVNLPGIPVDLPAV 763
NIT V++PG I++DDG++S + + V L + N G + S+KGVNLP VDLPA+
Sbjct: 139 NITKVIQPGRIIYVDDGILSFTVIEKVDDKNLKVRVNNNGKISSKKGVNLPKTDVDLPAL 198
Query: 764 SEKDKSDXLFGVXXGVDMI 820
SEKDK+D FGV GVDMI
Sbjct: 199 SEKDKADLRFGVKNGVDMI 217
>SPBC25B2.07c |mug164||microtubule-associated
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 29.1 bits (62), Expect = 0.90
Identities = 44/182 (24%), Positives = 71/182 (39%), Gaps = 3/182 (1%)
Frame = +2
Query: 182 QHMCGLDIDSKSSYIRLSGI--ICTIGPASRNVAVLE-KMMETGMNVARMNFSHGSHEYH 352
+H+ + S SS R+S I I A ++AV + G NV +
Sbjct: 148 KHVATITKPSTSSIARMSSNARIAAIPRAKSSMAVRSPSRLGNGPNVRSPKVGFNAKSDD 207
Query: 353 AETIRNCREAEKSYSAKLGSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTT 532
+ +++ +K ++ S SL + + P RT V G K +T
Sbjct: 208 SPVVKSPGSNDKPSASPRISVRSLGNSSVVR-PPTRTSTTRPLSRVNVTNASGSISKNST 266
Query: 533 SSDYQEKGNADTIYVDYKNITNVVKPGNRIFIDDGLISIICQSVSADTLTCTIENGGMLG 712
S + K NA T V + V+PG+RIF ++ + +VSA T T+ L
Sbjct: 267 SPS-KVKVNASTKIVRPVSAAQTVRPGSRIFRENSASNTQRPNVSA-TSNSTVRVASSLA 324
Query: 713 SR 718
R
Sbjct: 325 VR 326
>SPAC1B2.03c |||GNS1/SUR4 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 27.9 bits (59), Expect = 2.1
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -2
Query: 154 GLFNCTRESHGYVHRLIGLIYS*YFTVMLKVLN 56
GLF C +S + RL+ L Y Y T L++++
Sbjct: 118 GLFYCVCDSRHFTQRLVTLYYLNYLTKYLELMD 150
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 691 NGTRKSVSADRLTDDRDEAIIDEDSVSWLHDIR 593
N TRKS+S + TD + +D SWL +R
Sbjct: 576 NKTRKSISHSKNTDKHVGNELPQDIPSWLRSLR 608
>SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 791
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = +2
Query: 137 GAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIG 256
G + + ++ L+ + D+D+K +YI+LS T+G
Sbjct: 15 GTLNESSIYKTYQILESLNPKDVDTKENYIKLSNTFSTLG 54
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 26.6 bits (56), Expect = 4.8
Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 4/89 (4%)
Frame = -3
Query: 483 PP--PSKSPVLISGPLVSNAMAKEKGEPSLAL*LFSASRQLRMVSAWYSWEPCEKFILAT 310
PP PS+ P + + P + EPS+ L LR + + +P K L T
Sbjct: 430 PPKHPSRQPTVRATPSTGKQIEPPNDEPSIGNELLPLFESLRFENRKTTSKPKAKKTLVT 489
Query: 309 FIPVSIIFSSTATLRDAGPIVQI--IPER 229
IP +S + D + + IPE+
Sbjct: 490 SIPTKPHQASVEKVYDLNSKINLKNIPEQ 518
>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 4.8
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = -3
Query: 513 SPFFSSTSAEPPPSKSPVLISGPLVSNAMAKEKGEPSL 400
SP ST+A P K+P + L+S +KEK PS+
Sbjct: 305 SPLHQSTTANHP-EKTPSRAAASLLSILDSKEKNTPSI 341
>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1
Query: 565 YNLRGLQEHNECREARKPNLHR*W 636
++LRG H C EA+ PN+ W
Sbjct: 89 HDLRGSHLHTVCEEAKCPNIGECW 112
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 26.2 bits (55), Expect = 6.3
Identities = 14/65 (21%), Positives = 29/65 (44%)
Frame = +2
Query: 251 IGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKLGSPFSLAI 430
I P ++ + ++++ V R+N +H S ++ + SY +LG S I
Sbjct: 1194 ISPEAKTLLFQSIVIKSFSKVGRLNINHVSEPIDSDEFADYLSKSISYHLRLGKSKSKKI 1253
Query: 431 ALDTK 445
++ K
Sbjct: 1254 GIEIK 1258
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 199 RH*LQVFVHPSLRNNLYYWTGIP*CSCAR 285
RH L++ V L N+Y WT +P C +R
Sbjct: 2344 RHFLKLVV---LWKNIYVWTNVPDCDISR 2369
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 25.8 bits (54), Expect = 8.4
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -3
Query: 513 SPFFSSTSAEPPPSKSP 463
SP FSS+S PPP P
Sbjct: 231 SPIFSSSSTPPPPPPRP 247
>SPBC1A4.07c |||U3 snoRNP-associated protein
Sof1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 436
Score = 25.8 bits (54), Expect = 8.4
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1
Query: 694 KRRYARIPERRQPARHTXGPTRSLRKGQV 780
+ RY IPE R+ ARH PT + ++
Sbjct: 369 RERYKHIPEIRRIARHRHLPTNVKKAAEI 397
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,255,003
Number of Sequences: 5004
Number of extensions: 63213
Number of successful extensions: 221
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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