Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_F06
(878 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 261 1e-70
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 253 2e-68
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 132 6e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 107 2e-24
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 261 bits (639), Expect = 1e-70
Identities = 124/216 (57%), Positives = 149/216 (68%), Gaps = 2/216 (0%)
Frame = +1
Query: 124 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 303
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 304 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 483
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 484 RIRKLADQCTGLQGFLIFH--XXXXXXXXXXXXLDGASLR*LRQEV*TGVRHLPRASGST 657
+IR++AD C+GLQGFL+FH L+ ++ ++ P ST
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSK-LQFSVYPAPQVST 179
Query: 658 AVVEPYNSIXXXXXXXXXXXXCFMVDNEXIYDICRR 765
+VVEPYNS+ FMVDNE YDICRR
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 253 bits (620), Expect = 2e-68
Identities = 129/230 (56%), Positives = 154/230 (66%), Gaps = 7/230 (3%)
Frame = +1
Query: 124 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 288
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 289 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 468
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 469 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLDGASLR*LRQEV*TGVRH--LPR 642
D VL+RIR++AD C+GLQGFL+FH L L + + ++ P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN-MEYGKKSNLQFSVYPA 178
Query: 643 ASGSTAVVEPYNSIXXXXXXXXXXXXCFMVDNEXIYDICRR*SRH*APTY 792
ST+VVEPYNS+ FMVDNE YDICRR PTY
Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
Score = 49.6 bits (113), Expect = 6e-07
Identities = 30/77 (38%), Positives = 38/77 (49%)
Frame = +2
Query: 563 LGSLPLMERLSVDYGKKSKLEFAIYPAPQVXXXXXXXXXXXXXXXXXXXXXDCASWSTMX 742
LG+L L+ERL+++YGKKS L+F++YPAPQV DC
Sbjct: 153 LGAL-LLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211
Query: 743 PSMTSAAXNLDIERXPT 793
NLDIER PT
Sbjct: 212 ACYDICRRNLDIER-PT 227
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 132 bits (319), Expect = 6e-32
Identities = 76/224 (33%), Positives = 107/224 (47%), Gaps = 1/224 (0%)
Frame = +1
Query: 124 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 303
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 304 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 483
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 484 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLDGASLR-*LRQEV*TGVRHLPRASGSTA 660
+R+ A+ C LQGF + H L + +R + P S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 661 VVEPYNSIXXXXXXXXXXXXCFMVDNEXIYDICRR*SRH*APTY 792
VVEPYN+ F +DNE + I + +P+Y
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 107 bits (257), Expect = 2e-24
Identities = 68/214 (31%), Positives = 108/214 (50%), Gaps = 4/214 (1%)
Frame = +1
Query: 127 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 306
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 307 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 480
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 481 DRIRKLADQCTGLQGFLIFH--XXXXXXXXXXXXLDGASLR*LRQEV*TGVRHLPRASGS 654
D I + AD L+GF + H L+ + R ++ + T S S
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 655 TAVVEPYNSIXXXXXXXXXXXXCFMVDNEXIYDI 756
VV+PYNS+ ++DN + I
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 203 STASSLMARCPQTRPSGVETILSTLSSARPELAST 307
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 222 WPDAHRQDHRGWRRFFQHFLQR 287
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,515,854
Number of Sequences: 5004
Number of extensions: 72281
Number of successful extensions: 233
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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