Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_E22
(873 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 192 4e-50
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 165 1e-41
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 77 2e-15
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 34 0.030
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.21
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.28
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 29 0.65
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.5
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 2.6
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 3.5
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 6.1
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 26 8.1
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 192 bits (469), Expect = 4e-50
Identities = 93/130 (71%), Positives = 112/130 (86%)
Frame = +1
Query: 100 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 279
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 280 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 459
YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 460 RLFVGIFLFL 489
RLFV + L L
Sbjct: 125 RLFVAMILIL 134
Score = 39.1 bits (87), Expect = 8e-04
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = +3
Query: 477 ILILIFAEVLGLYGLIVAIYL 539
ILILIFAEVLGLYGLIVA+ L
Sbjct: 131 ILILIFAEVLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 165 bits (400), Expect = 1e-41
Identities = 75/130 (57%), Positives = 99/130 (76%)
Frame = +1
Query: 100 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 279
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 280 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 459
YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 460 RLFVGIFLFL 489
R+FV + L L
Sbjct: 127 RIFVSMVLIL 136
Score = 37.1 bits (82), Expect = 0.003
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = +3
Query: 477 ILILIFAEVLGLYGLIVAIYL 539
+LILIFAEVLGLYGLIV + L
Sbjct: 133 VLILIFAEVLGLYGLIVGLIL 153
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 77.4 bits (182), Expect = 2e-15
Identities = 39/128 (30%), Positives = 66/128 (51%), Gaps = 6/128 (4%)
Frame = +1
Query: 118 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 297
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 298 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 459
++ + + + PA + Y GF G+ VG L G +GI G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 460 RLFVGIFL 483
LFV + +
Sbjct: 165 SLFVKVLV 172
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = +3
Query: 477 ILILIFAEVLGLYGLIVAIYL 539
+++ IF VLGL+GLIV + +
Sbjct: 171 LVVEIFGSVLGLFGLIVGLLI 191
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 33.9 bits (74), Expect = 0.030
Identities = 25/74 (33%), Positives = 37/74 (50%)
Frame = +1
Query: 373 LAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGIFLFLFSLKYWVFTDLSSPSTCTQNK 552
L +GFS LA I I G A R + L +FL++ Y V TD +P+T + K
Sbjct: 225 LMIGFS-LATVIVISIYGVASGRANLSEASL---MFLYIAYTVYMVCTDFGNPNTSSLEK 280
Query: 553 RPEHTPLPSPVLRA 594
P+ LP +L++
Sbjct: 281 -PKFDYLPPNILQS 293
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.21
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +1
Query: 244 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 423
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 424 GDAGVRGT 447
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.28
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -2
Query: 449 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 348
+VP P++P MP P+A P A AP NP
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 29.5 bits (63), Expect = 0.65
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +1
Query: 466 FVGIFLFLFSLKYWVFTDLSSPSTCT 543
F GI++F+FS+ YW F +SS S T
Sbjct: 564 FCGIYVFIFSVFYWFF-KISSSSLAT 588
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.5
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -2
Query: 449 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 276
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 275 AIIPA 261
A PA
Sbjct: 479 APAPA 483
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 2.6
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +1
Query: 103 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 282
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 283 GLVVAVLIAGAL 318
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 3.5
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +1
Query: 79 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 243
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 244 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 345
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 6.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 357 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 244
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = -2
Query: 545 CVQVDGDD-KSV--KTQYFSENKNKNIPTNN 462
C++VD +D K + K+QY +EN N N N+
Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNS 263
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,605,483
Number of Sequences: 5004
Number of extensions: 76948
Number of successful extensions: 238
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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