Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_E14
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 172 5e-44
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 157 2e-39
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 31 0.28
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.5
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 27 3.4
SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomy... 26 5.9
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.9
SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 26 7.8
SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|... 26 7.8
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 172 bits (419), Expect = 5e-44
Identities = 82/127 (64%), Positives = 101/127 (79%)
Frame = +2
Query: 128 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGK 307
M I+ + ARQI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GK
Sbjct: 1 MAIQKVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGK 60
Query: 308 GVLTAIKNINELIAPELTKANLEVTQQRGIDELMLKLDGTENKSKLGANAILGVSLACC* 487
GVL A+ N+N +IAP + KANL+VT Q+ DE +LKLDGTENKSKLGANAILGVS+A C
Sbjct: 61 GVLKAVGNVNNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICR 120
Query: 488 GWCCQEE 508
Q++
Sbjct: 121 AGAAQKK 127
Score = 125 bits (302), Expect = 7e-30
Identities = 61/107 (57%), Positives = 78/107 (72%), Gaps = 1/107 (0%)
Frame = +3
Query: 510 VPLYKHLADLAGNND-IVLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEAMRMGS 686
+PL+K++A+ G VLPVP+FNV+NGGSHAG LA QEFMI PTGA +FSEAMR G+
Sbjct: 128 LPLWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGDLAFQEFMILPTGAPSFSEAMRWGA 187
Query: 687 EVYHHLKKIIKEKXGLDSTAVGDEGGXXPNIQNXKDALYLIQDAIXK 827
E YH LK I K++ G + VGDEGG P++Q ++AL LI +AI K
Sbjct: 188 ETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQEALDLIVEAINK 234
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 157 bits (380), Expect = 2e-39
Identities = 73/115 (63%), Positives = 94/115 (81%)
Frame = +2
Query: 134 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 313
I+ I +R I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV
Sbjct: 4 IQKIYSRSIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGV 63
Query: 314 LTAIKNINELIAPELTKANLEVTQQRGIDELMLKLDGTENKSKLGANAILGVSLA 478
A+ N+N +I P L K+++++T QRGIDE M+KLDGT +KSKLGAN+I+GVS+A
Sbjct: 64 TIAVHNVNNIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMA 118
Score = 117 bits (282), Expect = 2e-27
Identities = 55/105 (52%), Positives = 73/105 (69%), Gaps = 1/105 (0%)
Frame = +3
Query: 510 VPLYKHLADLAGNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEAMRMGS 686
+PLY+++ LAG+ +PVP+FNV+NGG HAG LA QEFMI P A TFSE +R GS
Sbjct: 129 IPLYEYIGKLAGSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEGLRWGS 188
Query: 687 EVYHHLKKIIKEKXGLDSTAVGDEGGXXPNIQNXKDALYLIQDAI 821
EVYH LK + K+K G + VGDEGG P++ ++AL L+ +AI
Sbjct: 189 EVYHTLKALAKKKYGASAGNVGDEGGIAPDLTTAEEALDLVNEAI 233
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 30.7 bits (66), Expect = 0.28
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -1
Query: 682 PILMASLKVDAPVGKIMNSCMASLFPACDPPLITLNAGTGRTMSL 548
PI+ S + + +G+ +N + LFP+CD LI G T+ L
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.5
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +2
Query: 164 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 343
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 344 IAPELTKANLEV 379
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 27.1 bits (57), Expect = 3.4
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = -2
Query: 573 LVQVEQCHYFQPNQPSACTAEHSSWQHQP*QQARETPRIALAPSLDLFSVPSNLSMS 403
L + E F +QPS + S P ++ + P+I+L S D+ + +++S S
Sbjct: 116 LSKYESTDSFPSSQPSRANSPQSDSYSSPYEKGKLFPKISLKSSKDVPTASAHISSS 172
>SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 538
Score = 26.2 bits (55), Expect = 5.9
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +2
Query: 425 TENKSKLGANAILGVSLACC*GWCCQEE 508
T + S + NA+L + + C GW C E
Sbjct: 199 TSSNSGINENAVLPILSSSCPGWICYVE 226
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.9
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = -2
Query: 444 SLDLFSVPSNLSMSSSIPLCWVTSRLAFVSSGAMSSLIFLIAVK 313
SL +F +PSNL M+ + P W+ SR+ V++G + + ++ K
Sbjct: 211 SLIIFDLPSNLLMTRADPRLWL-SRIQ-VTTGIIGACHAVLGTK 252
>SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase
Hrp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1373
Score = 25.8 bits (54), Expect = 7.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 78 QNCTLQAGFGKI*GFGCWRDYSSDP 4
++ L AG K GFGCW+ +DP
Sbjct: 1136 EDSMLLAGINKH-GFGCWQAIKNDP 1159
>SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = -2
Query: 549 YFQPNQPSACTAEHSSWQHQP*QQARETPRIALAPSLDLFSVPSNLSMSSSIPLCWVTSR 370
Y PN+ A +A S + + Q +P + PSL + S+P+ M + VTS
Sbjct: 399 YNPPNRNQAFSARDS--RQEKTQPTNASPAPLVTPSLPVPSIPAVPGMEAMAMPPGVTSS 456
Query: 369 LAFVSSGAM 343
+A ++ +M
Sbjct: 457 IAVPTTSSM 465
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,573,130
Number of Sequences: 5004
Number of extensions: 73142
Number of successful extensions: 185
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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