Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_E03
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 4e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29
SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces pom... 28 1.9
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.6
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.4
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.5
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 4e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 267
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 268 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 447
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 448 RKEAESCDCLQG 483
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 110 bits (265), Expect = 2e-25
Identities = 50/67 (74%), Positives = 58/67 (86%)
Frame = +2
Query: 548 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 727
KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFAN 213
Query: 728 TLKLSTP 748
TLK+ +P
Sbjct: 214 TLKIKSP 220
Score = 58.8 bits (136), Expect = 9e-10
Identities = 29/53 (54%), Positives = 36/53 (67%)
Frame = +3
Query: 690 VSTTRLSTISASAR*NYPHPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKL 848
+ LS+I A+ P+Y DLNHLVS M+GVTT RFPG+LN+DLRKL
Sbjct: 202 IDNEALSSIFANTL-KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKL 253
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 9e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 261
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 262 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 441
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 442 VVRKEAESCDCLQG 483
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 65.7 bits (153), Expect = 8e-12
Identities = 28/67 (41%), Positives = 43/67 (64%)
Frame = +2
Query: 548 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 727
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 728 TLKLSTP 748
L + P
Sbjct: 216 NLDIERP 222
Score = 37.1 bits (82), Expect = 0.003
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +3
Query: 747 PTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 839
P+Y +LN L++ +S +T LRF G LN DL
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDL 252
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 2e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 249
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 250 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 429
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 430 SVLDVVRKEAESCDCLQG 483
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 70.5 bits (165), Expect = 3e-13
Identities = 30/67 (44%), Positives = 45/67 (67%)
Frame = +2
Query: 548 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 727
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 728 TLKLSTP 748
L + P
Sbjct: 220 NLDIERP 226
Score = 39.1 bits (87), Expect = 8e-04
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +3
Query: 747 PTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRK 845
PTY +LN L++ +S +T LRF G LN DL +
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNE 258
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 2e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +1
Query: 91 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 270
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 271 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 444
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 445 VRKEAESCDCLQG 483
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 56.8 bits (131), Expect = 4e-09
Identities = 28/68 (41%), Positives = 39/68 (57%), Gaps = 1/68 (1%)
Frame = +2
Query: 548 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 724
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 725 RTLKLSTP 748
L P
Sbjct: 216 DRLHTQNP 223
Score = 39.1 bits (87), Expect = 8e-04
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = +3
Query: 720 ASAR*NYPHPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 839
A+ R + +PT+ N LVS MS TT LR+PG +N DL
Sbjct: 214 AADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
>SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 605
Score = 27.9 bits (59), Expect = 1.9
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = -2
Query: 847 SLRRSAFSCPGNRRHVVTPDMVSDTRWFRSPYVGCG*F*RAEADIVESLVVDTVG 683
SL + GN +V P + + W++S Y G F R V S + T+G
Sbjct: 136 SLHTTPDDLQGNGVNVPNPSLSASRSWYQSGYGISGFFNRIMTPSVNSQYISTIG 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 391 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 489
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 127 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 228
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 322 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 423
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -1
Query: 464 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 327
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 343 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 474
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,208,229
Number of Sequences: 5004
Number of extensions: 62190
Number of successful extensions: 196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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