Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_D21
(838 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 288 6e-79
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 281 9e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 4e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 129 4e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.6
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 288 bits (707), Expect = 6e-79
Identities = 126/186 (67%), Positives = 153/186 (82%)
Frame = +2
Query: 17 EYISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 196
E IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+V
Sbjct: 3 EIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYV 62
Query: 197 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 376
PR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+I
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 377 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 556
R++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VV
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVV 182
Query: 557 EPYNSI 574
EPYNS+
Sbjct: 183 EPYNSV 188
Score = 62.5 bits (145), Expect = 7e-11
Identities = 33/49 (67%), Positives = 36/49 (73%)
Frame = +1
Query: 658 NLDIERPTYTNLNRLIGQIVSSITASFEXSTAL*XVDLTEFXTNLVLTP 804
NLDIERP+Y NLNRLI Q+VSSITAS +L VDL EF TNLV P
Sbjct: 216 NLDIERPSYENLNRLIAQVVSSITASLRFEGSL-NVDLAEFQTNLVPYP 263
Score = 38.7 bits (86), Expect = 0.001
Identities = 26/79 (32%), Positives = 38/79 (48%), Gaps = 1/79 (1%)
Frame = +3
Query: 603 SDCAFMVDNEAIYDICRR*SRH*APNLHKPESSHRTDCLLDHCFF*XFDGAXXCGPHRVP 782
+DC FMVDNE+ YDICRR P+ ++ + + F+G+
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPS-YENLNRLIAQVVSSITASLRFEGSLNVDLAEFQ 256
Query: 783 X*LGAYP-VSLPLITYAPV 836
L YP + PL+TYAP+
Sbjct: 257 TNLVPYPRIHFPLVTYAPI 275
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 281 bits (689), Expect = 9e-77
Identities = 127/191 (66%), Positives = 155/191 (81%), Gaps = 5/191 (2%)
Frame = +2
Query: 17 EYISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETG 181
E ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG
Sbjct: 3 EVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETG 61
Query: 182 AGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDL 361
GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121
Query: 362 VLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 541
VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQV
Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181
Query: 542 STAVVEPYNSI 574
ST+VVEPYNS+
Sbjct: 182 STSVVEPYNSV 192
Score = 64.5 bits (150), Expect = 2e-11
Identities = 34/49 (69%), Positives = 37/49 (75%)
Frame = +1
Query: 658 NLDIERPTYTNLNRLIGQIVSSITASFEXSTAL*XVDLTEFXTNLVLTP 804
NLDIERPTY NLNRLI Q+VSSITAS + +L VDL EF TNLV P
Sbjct: 220 NLDIERPTYENLNRLIAQVVSSITASLRFAGSL-NVDLNEFQTNLVPYP 267
Score = 40.7 bits (91), Expect = 3e-04
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3
Query: 600 HSDCAFMVDNEAIYDICRR 656
+SDC FMVDNEA YDICRR
Sbjct: 201 NSDCTFMVDNEACYDICRR 219
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 146 bits (353), Expect = 4e-36
Identities = 71/185 (38%), Positives = 102/185 (55%)
Frame = +2
Query: 17 EYISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 196
E + + GQ G Q+G A W EHG+ G T + N +F+E GK+V
Sbjct: 3 EIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYV 60
Query: 197 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 376
PRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 377 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 556
R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S VV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 557 EPYNS 571
EPYN+
Sbjct: 181 EPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 129 bits (312), Expect = 4e-31
Identities = 70/189 (37%), Positives = 108/189 (57%), Gaps = 3/189 (1%)
Frame = +2
Query: 17 EYISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 196
E I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++
Sbjct: 4 EIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYI 61
Query: 197 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLD 370
PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 371 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VST 547
I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSD 180
Query: 548 AVVEPYNSI 574
VV+PYNS+
Sbjct: 181 VVVQPYNSL 189
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.6
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 90 STASSLMARCPQTRPSGVETILSTLSSARPELAST 194
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 109 WPDAHRQDHRGWRRFFQHFLQR 174
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,398,865
Number of Sequences: 5004
Number of extensions: 68217
Number of successful extensions: 194
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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