Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_B13
(927 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 177 1e-45
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 162 6e-41
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 96 7e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 6e-14
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.057
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 30 0.53
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 26 0.96
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.1
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 28 2.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 2.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.6
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 6.6
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 177 bits (432), Expect = 1e-45
Identities = 83/150 (55%), Positives = 100/150 (66%)
Frame = +3
Query: 231 HXGXTXXEMGXACFLXXXREHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 410
H G ++G AC+ EHGIQP+G M + D F+TFFSETG GK+VPR+++
Sbjct: 8 HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67
Query: 411 VDLEPTVVDEVRTGTYRQLXHPXQLITGKEDAANXYAXGPXTXGKEXXXLVXDXLRXLAD 590
VDLEP V+D+VRTG YR L HP QLITGKEDA+N YA G T GKE V D +R +AD
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 591 XCTGLQGXLIFHSFXAGTXSGFXXLLXXXL 680
C+GLQG L+FHSF GT SGF LL L
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERL 157
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 162 bits (394), Expect = 6e-41
Identities = 80/155 (51%), Positives = 99/155 (63%), Gaps = 5/155 (3%)
Frame = +3
Query: 231 HXGXTXXEMGXACFLXXXREHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 395
H G ++G AC+ EHGI PDG PT+ K +D F TFFSETG GK V
Sbjct: 8 HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66
Query: 396 PRAVFVDLEPTVVDEVRTGTYRQLXHPXQLITGKEDAANXYAXGPXTXGKEXXXLVXDXL 575
PR+++VDLEP V+D+VRTG Y+ L HP Q++TGKEDA+N YA G T GKE V + +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 576 RXLADXCTGLQGXLIFHSFXAGTXSGFXXLLXXXL 680
R +AD C+GLQG L+FHSF GT SG LL L
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERL 161
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 95.9 bits (228), Expect = 7e-21
Identities = 53/144 (36%), Positives = 71/144 (49%)
Frame = +3
Query: 237 GXTXXEMGXACFLXXXREHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 416
G ++G A + EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 417 LEPTVVDEVRTGTYRQLXHPXQLITGKEDAANXYAXGPXTXGKEXXXLVXDXLRXLADXC 596
LEP +D V++G + L P +I G+ A N +A G T G E V D +R A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 597 TGLQGXLIFHSFXAGTXSGFXXLL 668
LQG + HS GT SG LL
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLL 151
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.9 bits (171), Expect = 6e-14
Identities = 45/150 (30%), Positives = 71/150 (47%), Gaps = 2/150 (1%)
Frame = +3
Query: 237 GXTXXEMGXACFLXXXREHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 416
G ++G + EHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 417 LEPTVVDEVRTGTYRQLXHPXQLITGKE--DAANXYAXGPXTXGKEXXXLVXDXLRXLAD 590
LEP VV+ + + TY L +P ++ K A N +A G + + + D + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 591 XCTGLQGXLIFHSFXAGTXSGFXXLLXXXL 680
L+G + HS GT SG L L
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERL 157
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.1 bits (72), Expect = 0.057
Identities = 23/61 (37%), Positives = 23/61 (37%)
Frame = +3
Query: 729 PPPPXXHXPPXXXXXTLXSXPTXXPXXPXNXPXXGXXPPPPXPPPPXXLEXGPRXXPNXX 908
PPPP PP T P P P P G PPPP PPP GP P
Sbjct: 732 PPPP----PPAVIVPT--PAPAPIPVPPP-APIMGGPPPPP--PPPGVAGAGPPPPPPPP 782
Query: 909 P 911
P
Sbjct: 783 P 783
Score = 32.7 bits (71), Expect = 0.075
Identities = 19/58 (32%), Positives = 19/58 (32%), Gaps = 4/58 (6%)
Frame = +3
Query: 729 PPPPXXHXPPXXXXXTLXSXPTXXPXXPXNXPXX----GXXPPPPXPPPPXXLEXGPR 890
PPPP P P P P G PPPP PPPP G R
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSR 791
Score = 25.8 bits (54), Expect = 8.7
Identities = 10/25 (40%), Positives = 11/25 (44%)
Frame = +3
Query: 849 PXPPPPXXLEXGPRXXPNXXPPXXP 923
P PPPP + P P PP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAP 756
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 29.9 bits (64), Expect = 0.53
Identities = 11/24 (45%), Positives = 12/24 (50%)
Frame = +3
Query: 843 PPPXPPPPXXLEXGPRXXPNXXPP 914
PPP PPPP L P+ P P
Sbjct: 370 PPPPPPPPELLNHSPKSRPLGDKP 393
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.2 bits (55), Expect(2) = 0.96
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +3
Query: 840 PPPPXPPPP 866
PPPP PPPP
Sbjct: 945 PPPPPPPPP 953
Score = 21.0 bits (42), Expect(2) = 0.96
Identities = 8/21 (38%), Positives = 9/21 (42%)
Frame = +3
Query: 801 PXXPXNXPXXGXXPPPPXPPP 863
P + P P PP PPP
Sbjct: 892 PNDATSLPTIITHPTPPPPPP 912
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/47 (38%), Positives = 18/47 (38%), Gaps = 2/47 (4%)
Frame = -3
Query: 922 GXXGGXXLGXXRGPXSRXXGGGGXGG--GGXXPXXGXFXGXXGXXVG 788
G GG G GP G GG GG GG G F G G G
Sbjct: 224 GGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270
Score = 27.1 bits (57), Expect = 3.8
Identities = 18/48 (37%), Positives = 18/48 (37%), Gaps = 2/48 (4%)
Frame = -3
Query: 925 EGXXGGXXLGXXRGPXSRXXGGGGXGG--GGXXPXXGXFXGXXGXXVG 788
EG G G GP G GG GG GG G F G G G
Sbjct: 216 EGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGG 263
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.1
Identities = 16/53 (30%), Positives = 16/53 (30%)
Frame = +3
Query: 729 PPPPXXHXPPXXXXXTLXSXPTXXPXXPXNXPXXGXXPPPPXPPPPXXLEXGP 887
P P PP T P P P P PP PPPP P
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG--PPSAPPPPLPASSAP 1741
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +3
Query: 840 PPPPXPPPPXXLEXGPRXXPNXXPPXXPS 926
PPP PPP P P+ PP P+
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPPLPA 1737
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 27.9 bits (59), Expect = 2.1
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +3
Query: 831 GXXPPPPXPPPP 866
G PPPP PPPP
Sbjct: 303 GGLPPPPPPPPP 314
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/43 (34%), Positives = 18/43 (41%), Gaps = 2/43 (4%)
Frame = +3
Query: 777 LXSXPTXXPXXPXNXPXXGXXPP--PPXPPPPXXLEXGPRXXP 899
+ S P P P P G PP PP PPPP ++ P
Sbjct: 1 MASLPPGNPPPPP--PPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/27 (48%), Positives = 13/27 (48%), Gaps = 2/27 (7%)
Frame = +3
Query: 840 PP--PPXPPPPXXLEXGPRXXPNXXPP 914
PP PP PPPP E P P PP
Sbjct: 5 PPGNPPPPPPPPGFEP-PSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.6
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +3
Query: 840 PPPPXPPPP 866
PPPP PPPP
Sbjct: 189 PPPPPPPPP 197
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 6.6
Identities = 17/48 (35%), Positives = 18/48 (37%), Gaps = 1/48 (2%)
Frame = +3
Query: 783 SXPTXXPXXPXNXPXXGXXPPPPXPPPPXXLEXGPRXXPNXXP-PXXP 923
S P+ P P P PPP PPP P PN P P P
Sbjct: 235 SIPSSRP--PERVPSLSAPAPPPIPPPSNGTVSSP---PNSPPRPIAP 277
Score = 25.8 bits (54), Expect = 8.7
Identities = 21/78 (26%), Positives = 23/78 (29%), Gaps = 13/78 (16%)
Frame = +3
Query: 729 PPPPXX------HXPPXXXXXTLXSXPTXXPXXPXNX----PXXGXXP---PPPXPPPPX 869
PPPP PP + + P P P P G PP P PP
Sbjct: 363 PPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPS 422
Query: 870 XLEXGPRXXPNXXPPXXP 923
P P PP P
Sbjct: 423 LPPSAPPSLPPSAPPSLP 440
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 289 STASSLMARCPQTRPSGVETILSTLSSARPELAST 393
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 308 WPDAHRQDHRGWRRFFQHFLQR 373
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,410,233
Number of Sequences: 5004
Number of extensions: 36000
Number of successful extensions: 184
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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