Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP03_FL5_A02
         (844 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   242   4e-65
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   234   1e-62
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   127   2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   109   5e-25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.7  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.7  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  242 bits (593), Expect = 4e-65
 Identities = 111/188 (59%), Positives = 133/188 (70%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXXXXXXXXXXXXQEV*TGVRHLPRASGSTA 645
           +IR++AD C+GLQGFL+FH                       ++        P    ST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 646 VVEPYNSI 669
           VVEPYNS+
Sbjct: 181 VVEPYNSV 188



 Score = 71.7 bits (168), Expect = 1e-13
 Identities = 32/38 (84%), Positives = 35/38 (92%)
 Frame = +3

Query: 711 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQMVSSIT 824
           FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSIT
Sbjct: 202 FMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  234 bits (572), Expect = 1e-62
 Identities = 112/193 (58%), Positives = 135/193 (69%), Gaps = 5/193 (2%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXXXXXXXXXXXXQEV*TGVRHLPRA 630
           D VL+RIR++AD C+GLQGFL+FH                       ++        P  
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 631 SGSTAVVEPYNSI 669
             ST+VVEPYNS+
Sbjct: 180 QVSTSVVEPYNSV 192



 Score = 74.5 bits (175), Expect = 2e-14
 Identities = 34/38 (89%), Positives = 35/38 (92%)
 Frame = +3

Query: 711 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQMVSSIT 824
           FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSIT
Sbjct: 206 FMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSIT 243


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  127 bits (306), Expect = 2e-30
 Identities = 67/187 (35%), Positives = 91/187 (48%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXXXXXXXXXXXXQEV*TGVRHLPRASGSTA 645
            +R+ A+ C  LQGF + H                         +       P    S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 646 VVEPYNS 666
           VVEPYN+
Sbjct: 179 VVEPYNA 185



 Score = 37.1 bits (82), Expect = 0.003
 Identities = 15/41 (36%), Positives = 25/41 (60%)
 Frame = +3

Query: 711 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQMVSSITXFF 833
           F +DNEA+  I    L I+ P+Y +LN L+  +++ +T  F
Sbjct: 200 FCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  109 bits (262), Expect = 5e-25
 Identities = 63/190 (33%), Positives = 99/190 (52%), Gaps = 3/190 (1%)
 Frame = +1

Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXXXXXXXXXXXXQEV*TGVRHLPRA-SGS 639
           D I + AD    L+GF + H                       +++       P + S S
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 640 TAVVEPYNSI 669
             VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189



 Score = 26.2 bits (55), Expect = 5.8
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = +3

Query: 714 MVDNEAIYDICRRNLDIERPTYTNLNRLIGQMVSSIT 824
           ++DN A+  I    L  + PT+   N+L+  ++S+ T
Sbjct: 204 VLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAST 240


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +2

Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,460,062
Number of Sequences: 5004
Number of extensions: 72447
Number of successful extensions: 198
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)