Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_T7_M04
(837 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 338 7e-94
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 334 7e-93
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 137 2e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 4e-14
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.20
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.27
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.4
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 1.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.3
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.8
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.8
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 7.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 338 bits (830), Expect = 7e-94
Identities = 149/189 (78%), Positives = 168/189 (88%)
Frame = -2
Query: 695 DLTEFQXXLVPYPRIXFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHG 516
DL EFQ LVPYPRI FPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR G
Sbjct: 255 DLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTG 314
Query: 515 KYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAK 336
+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AK
Sbjct: 315 RYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAK 374
Query: 335 VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEK 156
V RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+
Sbjct: 375 VNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALER 434
Query: 155 DYEEVGMDS 129
DYEEVG DS
Sbjct: 435 DYEEVGQDS 443
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 334 bits (822), Expect = 7e-93
Identities = 149/189 (78%), Positives = 166/189 (87%)
Frame = -2
Query: 695 DLTEFQXXLVPYPRIXFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHG 516
DL EFQ LVPYPRI FPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR G
Sbjct: 251 DLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAG 310
Query: 515 KYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAK 336
+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AK
Sbjct: 311 RYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAK 370
Query: 335 VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEK 156
V RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+
Sbjct: 371 VDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALER 430
Query: 155 DYEEVGMDS 129
DYEEVG DS
Sbjct: 431 DYEEVGQDS 439
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 137 bits (332), Expect = 2e-33
Identities = 69/191 (36%), Positives = 110/191 (57%), Gaps = 3/191 (1%)
Frame = -2
Query: 695 DLTEFQXXLVPYPRIXFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHG 516
DL + +VP+PR+ F +V +AP+ + + + +SV E+T F+ N MV DPRHG
Sbjct: 249 DLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHG 308
Query: 515 KYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAK 336
+Y+ L+RG V K+V+ I +++TK + FV+W P + PP DL
Sbjct: 309 RYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL-- 361
Query: 335 VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAA 165
+ + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL +
Sbjct: 362 -KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
Query: 164 LEKDYEEVGMD 132
+ Y+E G+D
Sbjct: 421 EYQQYQEAGID 431
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 73.3 bits (172), Expect = 4e-14
Identities = 49/190 (25%), Positives = 91/190 (47%), Gaps = 7/190 (3%)
Frame = -2
Query: 695 DLTEFQXXLVPYPRIXFPLVTYAPVISA---EKAYHEQLSVAEITNACFEPANQMVKCDP 525
DL L+P PR F L +Y P + E + +V ++ P NQMV +P
Sbjct: 252 DLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311
Query: 524 -RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 348
+ +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P +
Sbjct: 312 SKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNH 371
Query: 347 DLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEARE 177
++ + ML+N T+IA + R ++D + + AF+ Y E + E EF +R+
Sbjct: 372 RVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRD 426
Query: 176 DLAALEKDYE 147
+A L +YE
Sbjct: 427 VVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 31.1 bits (67), Expect = 0.20
Identities = 24/89 (26%), Positives = 33/89 (37%)
Frame = -3
Query: 619 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 440
S+P+ P P S + P P + S VV P + RP++P
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPV-----APEVPSAPQRPAAPVVPEAPSVPQRPAVPV 567
Query: 439 KPSVLSNSSTGVQPVSRSVSTTSHPPWCP 353
P LS V PV+ V + PP P
Sbjct: 568 VPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.7 bits (66), Expect = 0.27
Identities = 25/91 (27%), Positives = 39/91 (42%), Gaps = 2/91 (2%)
Frame = -3
Query: 631 TRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*T 458
T S+ P PT+ S +P + T+ ++ + +TPV ++ CT TS P T
Sbjct: 402 TGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--T 459
Query: 457 RPSLPSKPSVLSNSSTGVQPVSRSVSTTSHP 365
+ S P +N +T S TS P
Sbjct: 460 STPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490
Score = 27.5 bits (58), Expect = 2.5
Identities = 29/105 (27%), Positives = 47/105 (44%)
Frame = -3
Query: 685 SSRXXWCLTPVFXSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTW 506
S+ + TPV S++ T SS P T P + T+ ++ + +TPV ++
Sbjct: 499 STSVPYTSTPVTSSNY--TISSSTPVTST------PVTTTNCTTSTSVLYTSTPVTSTPL 550
Query: 505 LAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 371
CT TS P T P S ++ S++ PV+ + TTS
Sbjct: 551 ATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = -2
Query: 575 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 450
+ ACFEP N C H K C L + KD N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 447 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 590
S G +LGY ++ A++V+A + V HL+ G D + K
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 3.3
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 631 TRQSSLPRRPTMNSFPSPRSQTHASSPPTR 542
T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1251 TPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 5.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 625 QSSLPRRPTMNSFPSPRSQTHASSPPTR 542
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.8
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -3
Query: 451 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 365
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +2
Query: 470 WVRRHHGTAYSKPCTCHDGG 529
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,858,673
Number of Sequences: 5004
Number of extensions: 58860
Number of successful extensions: 216
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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