Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_T7_E02
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 246 3e-66
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 241 6e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 3e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 1e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.25
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 29 0.77
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.3
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.4
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 9.5
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.5
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 25 9.5
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 246 bits (602), Expect = 3e-66
Identities = 107/133 (80%), Positives = 122/133 (91%)
Frame = -2
Query: 532 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 353
PR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG
Sbjct: 311 PRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGS 370
Query: 352 DLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLA 173
+AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLA
Sbjct: 371 GIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLA 430
Query: 172 ALEKDYEEVGMDS 134
ALE+DYEEVG DS
Sbjct: 431 ALERDYEEVGQDS 443
Score = 87.0 bits (206), Expect = 3e-18
Identities = 37/48 (77%), Positives = 41/48 (85%)
Frame = -3
Query: 702 DLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 559
DL EFQ NLVPYPRIHFPLVTY+P++SA KA+HE SV EITN CFEP
Sbjct: 255 DLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 27.5 bits (58), Expect = 2.3
Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 2/74 (2%)
Frame = -1
Query: 746 SSIXXXLRXXGALNVTSPSSRXTWCLTPVSTSHWSRTRQSSL-PRRPTMNSFPSPRSQTH 570
SSI LR G+LNV + L P H+ S + + S + T+
Sbjct: 240 SSITASLRFAGSLNVDLNEFQTN--LVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITN 297
Query: 569 ASSPP-NQMVKCDP 531
P NQMVKCDP
Sbjct: 298 QCFEPYNQMVKCDP 311
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 241 bits (591), Expect = 6e-65
Identities = 106/133 (79%), Positives = 120/133 (90%)
Frame = -2
Query: 532 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 353
PR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G
Sbjct: 307 PRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGS 366
Query: 352 DLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLA 173
++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLA
Sbjct: 367 EIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLA 426
Query: 172 ALEKDYEEVGMDS 134
ALE+DYEEVG DS
Sbjct: 427 ALERDYEEVGQDS 439
Score = 89.0 bits (211), Expect = 7e-19
Identities = 42/68 (61%), Positives = 48/68 (70%)
Frame = -3
Query: 702 DLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPAKPDGEMRPPVM 523
DL EFQ NLVPYPRIHFPLVTYAP++SA KA+HE SV EITN CFEP + P
Sbjct: 251 DLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP--R 308
Query: 522 ASTWLAVC 499
A ++A C
Sbjct: 309 AGRYMATC 316
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 100 bits (239), Expect = 3e-22
Identities = 51/137 (37%), Positives = 79/137 (57%), Gaps = 3/137 (2%)
Frame = -2
Query: 538 ATPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVP 359
A PRHG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 303 ADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK--- 359
Query: 358 GGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR-- 185
DL + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 --DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESN 414
Query: 184 -EDLAALEKDYEEVGMD 137
DL + + Y+E G+D
Sbjct: 415 MNDLVSEYQQYQEAGID 431
Score = 42.3 bits (95), Expect = 8e-05
Identities = 17/47 (36%), Positives = 30/47 (63%)
Frame = -3
Query: 702 DLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 562
DL + N+VP+PR+HF +V +AP+ + + + +SV E+T F+
Sbjct: 249 DLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFD 295
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.0 bits (119), Expect = 1e-07
Identities = 31/119 (26%), Positives = 61/119 (51%), Gaps = 3/119 (2%)
Frame = -2
Query: 499 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 320
+ +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ + M
Sbjct: 323 IIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----M 377
Query: 319 LSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEAREDLAALEKDYE 152
L+N T+IA + R ++D + + AF+ Y E + E EF +R+ +A L +YE
Sbjct: 378 LANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
Score = 28.3 bits (60), Expect = 1.3
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -3
Query: 702 DLTEFQXNLVPYPRIHFPLVTYAP 631
DL +L+P PR HF L +Y P
Sbjct: 252 DLVSIIASLIPSPRCHFLLTSYTP 275
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 30.7 bits (66), Expect = 0.25
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -3
Query: 492 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 358
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 25.4 bits (53), Expect = 9.5
Identities = 26/106 (24%), Positives = 36/106 (33%)
Frame = -3
Query: 675 VPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPAKPDGEMRPPVMASTWLAVCC 496
VP P + P+V AP + V + P P+ P A+
Sbjct: 589 VPQPPVA-PVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAP------ 641
Query: 495 TVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 358
VV P + RP++P P S PV V + PP P
Sbjct: 642 -VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 29.1 bits (62), Expect = 0.77
Identities = 15/56 (26%), Positives = 24/56 (42%)
Frame = +1
Query: 475 WVRRHHGTAYSKPCTCHDGGSHFTIWFGGLEACVCDLGDGKLFMVGLLGRDDWRVR 642
W R H Y+KPC DGG W G+ +G L+ + + +++ R
Sbjct: 16 WRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTR 71
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.3
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -3
Query: 288 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 115
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 114 EPKSTK 97
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -3
Query: 456 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 370
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 632 QSSLPRRPTMNSFPSPRSQTHASSPP 555
+ SLPRRP+ +P S T ++ PP
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPP 763
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -3
Query: 459 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 364
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.4 bits (53), Expect = 9.5
Identities = 16/65 (24%), Positives = 26/65 (40%)
Frame = -3
Query: 567 FEPAKPDGEMRPPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSV 388
F A P + PP + + V P + RP+L S+ S S + PV++
Sbjct: 73 FSHAAPLHPLSPPSFTHSQPQIQAQPVPRRPSLFDRPNLVSRSSSRLGDSPSLSPVAQVA 132
Query: 387 STTSH 373
+ H
Sbjct: 133 NPIHH 137
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,914,438
Number of Sequences: 5004
Number of extensions: 63537
Number of successful extensions: 255
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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