Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_T7_D19
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 135 6e-33
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 52 9e-08
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 48 1e-06
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 36 0.005
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 31 0.25
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 29 0.76
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.3
SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 27 3.1
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 27 4.0
SPBC3E7.02c |hsp16||heat shock protein Hsp16|Schizosaccharomyces... 27 4.0
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 135 bits (327), Expect = 6e-33
Identities = 56/78 (71%), Positives = 66/78 (84%)
Frame = -2
Query: 348 GEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFGSVPHGGYGLGLERFLCWLLD 169
GEIVGGSMRI D +EL+ YKREGIDP+PYYW+T+QRK+G+ HGG GLGLERFL WL D
Sbjct: 490 GEIVGGSMRISDIQELLAAYKREGIDPAPYYWFTEQRKYGTTEHGGCGLGLERFLAWLCD 549
Query: 168 RYHIRDVCLYPRFLERCT 115
RY +R+ CL+PRF ERCT
Sbjct: 550 RYTVRECCLFPRFTERCT 567
Score = 93.9 bits (223), Expect = 2e-20
Identities = 46/80 (57%), Positives = 55/80 (68%)
Frame = -1
Query: 589 RMTYIEAIEYLRANNITKEDGSFYEFGEDIPEMPERKMTDAIGVPILLCKFPAEIKSFYM 410
R++Y +AI+YL +NI +G ++FG+DI E ERKMTD I PI L FP EIKSFYM
Sbjct: 410 RLSYEDAIKYLNEHNILTPEGEQHKFGDDIAEAAERKMTDQINRPIFLTYFPLEIKSFYM 469
Query: 409 SRCVDDRRLTESVDVLMPGV 350
R VD LTESVD LMP V
Sbjct: 470 KRVVDRPELTESVDCLMPNV 489
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 52.0 bits (119), Expect = 9e-08
Identities = 27/73 (36%), Positives = 43/73 (58%), Gaps = 1/73 (1%)
Frame = -2
Query: 348 GEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFGSVPHGGYGLGLERFLCWLL- 172
GE+ GGS+R DH ++ YK P WY D K+ + PHGG+GLG+ER + +L
Sbjct: 369 GELAGGSLRK-DH---LDEYKTY---PPELQWYLDLMKYSNAPHGGFGLGIERLIAFLEG 421
Query: 171 DRYHIRDVCLYPR 133
+ ++++ +PR
Sbjct: 422 ENTNVKETIPFPR 434
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 48.4 bits (110), Expect = 1e-06
Identities = 28/72 (38%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = -2
Query: 345 EIVGGSMRIWDHEELMEGYKREGIDPSP-YYWYTDQRKFGSVPHGGYGLGLERFLCWLLD 169
EI+ G+ RI D E L+E K G+ P Y D G PH G G+GLER + + L+
Sbjct: 502 EIMSGAQRIHDPELLVERMKALGVSPDVGLQQYIDAFAIGCPPHAGGGIGLERVVMFYLN 561
Query: 168 RYHIRDVCLYPR 133
+IR +PR
Sbjct: 562 LPNIRLASSFPR 573
Score = 27.5 bits (58), Expect = 2.3
Identities = 12/42 (28%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -1
Query: 442 KFPAEIKSFYMSRCVDDRRLTESVDVLMPG--VRGDRRRLHE 323
K+P+ ++ FY +D R + S D M G + +R+H+
Sbjct: 471 KYPSSVRPFYTMPDPEDPRYSNSYDFFMKGQEIMSGAQRIHD 512
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -2
Query: 237 KFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYP 136
++G P GG+G+G++R + +L D IR+V L+P
Sbjct: 542 EYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 30.7 bits (66), Expect = 0.25
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 231 GSVPHGGYGLGLERFLCWLLDRYHIRDVCLYPR 133
G PHGG LG +R L + IR+V +P+
Sbjct: 544 GCPPHGGIALGFDRLAALLTNAPGIREVIAFPK 576
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 29.1 bits (62), Expect = 0.76
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +1
Query: 439 TCTAVSARQWRPSSSALAFPGCPLQTRRRI 528
TC + R W S FP CP + RR+
Sbjct: 1152 TCKMIEKRLWPTMSPLRQFPNCPSEVIRRV 1181
>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 781
Score = 27.5 bits (58), Expect = 2.3
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -2
Query: 234 FGSVPHGGYGLGLERFLCWLLD 169
FG+ P Y + F+CWL D
Sbjct: 201 FGTSPFNAYQQNTDNFVCWLFD 222
>SPBP8B7.07c |set6||histone lysine methyltransferase Set6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = -2
Query: 234 FGSVPH---GGYGLGLERFLCWLLDR 166
FG + H G Y + L+ F+ WLLDR
Sbjct: 396 FGGLHHCILGRYSISLDDFVLWLLDR 421
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +1
Query: 130 EARVETHVPDVVPVQEPAEEALQTQPVAAVRHRAELA 240
+ R + +PD+V V +P EE T+P + E A
Sbjct: 196 KTRQKKSLPDIVVVLDPKEEEPITKPYTVINQPVEAA 232
>SPBC3E7.02c |hsp16||heat shock protein Hsp16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 26.6 bits (56), Expect = 4.0
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 400 VDDRRLTESVDVLMPGVRGDRRRLHEDMGPRGADGRLQARR 278
V + + T SVDV +PGV+ + ++H D G G + R
Sbjct: 41 VHEGKDTVSVDVELPGVKKEDVQVHYDSGKLTISGEVVNER 81
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,679,550
Number of Sequences: 5004
Number of extensions: 49836
Number of successful extensions: 136
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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