Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_T7_B03
(810 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 227 2e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 223 2e-59
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 1e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.012
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.5
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 5.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 7.3
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 7.3
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 26 7.3
SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharom... 25 9.6
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 9.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 227 bits (554), Expect = 2e-60
Identities = 120/196 (61%), Positives = 141/196 (71%), Gaps = 9/196 (4%)
Frame = -1
Query: 690 EFQTNLVPYPP--YPLPT-----GHVRASHLCREGLP*TAFRRRDHKRMLRA--RQXRW* 538
EFQTNLVPYP +PL T +A H T + +M++ R R+
Sbjct: 258 EFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRY- 316
Query: 537 NATPVMASTWLAVRCTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVV 358
MA+ L RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP V
Sbjct: 317 -----MATCLLY----RGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHV 367
Query: 357 PGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEARE 178
PG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEARE
Sbjct: 368 PGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEARE 427
Query: 177 DLAALEKDYEEVGMDS 130
DLAALE+DYEEVG DS
Sbjct: 428 DLAALERDYEEVGQDS 443
Score = 65.7 bits (153), Expect = 7e-12
Identities = 27/36 (75%), Positives = 31/36 (86%)
Frame = -2
Query: 662 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 555
PRIHFPLVTY+P++SA KA+HE SV EITN CFEP
Sbjct: 267 PRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 59.7 bits (138), Expect = 5e-10
Identities = 39/92 (42%), Positives = 48/92 (52%), Gaps = 2/92 (2%)
Frame = -3
Query: 760 IGQIVSSITASXRFDGALNVDLTRVPD*LGALPPVSTSHWSRTRQSSL-PRRPTMNSFPS 584
I Q+VSSITAS RF G+LNVDL L P + H+ S + + S
Sbjct: 235 IAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRI---HFPLVTYSPIVSAAKAFHESNS 291
Query: 583 PRSQTHASSPPX-QMVKCDPRHGKYMACCTLY 491
+ T+ P QMVKCDPR G+YMA C LY
Sbjct: 292 VQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 223 bits (546), Expect = 2e-59
Identities = 118/194 (60%), Positives = 137/194 (70%), Gaps = 7/194 (3%)
Frame = -1
Query: 690 EFQTNLVPYPP--YPLPT-----GHVRASHLCREGLP*TAFRRRDHKRMLRARQXRW*NA 532
EFQTNLVPYP +PL T +A H T + +M++ A
Sbjct: 254 EFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP----RA 309
Query: 531 TPVMASTWLAVRCTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPG 352
MA+ L RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G
Sbjct: 310 GRYMATCLLY----RGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEG 365
Query: 351 GDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDL 172
++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDL
Sbjct: 366 SEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDL 425
Query: 171 AALEKDYEEVGMDS 130
AALE+DYEEVG DS
Sbjct: 426 AALERDYEEVGQDS 439
Score = 66.9 bits (156), Expect = 3e-12
Identities = 28/36 (77%), Positives = 31/36 (86%)
Frame = -2
Query: 662 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 555
PRIHFPLVTYAP++SA KA+HE SV EITN CFEP
Sbjct: 263 PRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298
Score = 60.1 bits (139), Expect = 4e-10
Identities = 38/92 (41%), Positives = 49/92 (53%), Gaps = 2/92 (2%)
Frame = -3
Query: 760 IGQIVSSITASXRFDGALNVDLTRVPD*LGALPPVSTSHWSRTRQSSL-PRRPTMNSFPS 584
I Q+VSSITAS RF+G+LNVDL L P + H+ + + + S
Sbjct: 231 IAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRI---HFPLVTYAPIVSAAKAFHESNS 287
Query: 583 PRSQTHASSPPX-QMVKCDPRHGKYMACCTLY 491
+ T+ P QMVKCDPR G+YMA C LY
Sbjct: 288 VQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 82.2 bits (194), Expect = 8e-17
Identities = 44/122 (36%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = -1
Query: 489 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 310
RG V K+V+ I +++TK + FV+W P + PP DL + + +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIG 369
Query: 309 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVG 139
N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y+E G
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 138 MD 133
+D
Sbjct: 430 ID 431
Score = 33.5 bits (73), Expect = 0.036
Identities = 22/92 (23%), Positives = 38/92 (41%), Gaps = 2/92 (2%)
Frame = -3
Query: 760 IGQIVSSITASXRFDGALNVDLTRVPD*LGALPPVSTSHWSRTRQSSLPR--RPTMNSFP 587
+ +++ +T S RF G LN DL ++ + P + H+ + L + +
Sbjct: 229 VSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRL---HFFMVGFAPLAAIGSSSFQAVS 285
Query: 586 SPRSQTHASSPPXQMVKCDPRHGKYMACCTLY 491
P MV DPRHG+Y+ L+
Sbjct: 286 VPELTQQMFDANNMMVAADPRHGRYLTVAALF 317
Score = 30.3 bits (65), Expect = 0.34
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -2
Query: 662 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 558
PR+HF +V +AP+ + + + +SV E+T F+
Sbjct: 261 PRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFD 295
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 51.6 bits (118), Expect = 1e-07
Identities = 31/117 (26%), Positives = 60/117 (51%), Gaps = 3/117 (2%)
Frame = -1
Query: 489 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 310
+G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ + ML+
Sbjct: 325 QGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----MLA 379
Query: 309 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEAREDLAALEKDYE 148
N T+IA + R ++D + + AF+ Y E + E EF +R+ +A L +YE
Sbjct: 380 NHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 35.1 bits (77), Expect = 0.012
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -2
Query: 497 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 354
A PVV P + RP++P P LS V PV+ V + PP P
Sbjct: 549 AAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 31.5 bits (68), Expect = 0.15
Identities = 26/96 (27%), Positives = 37/96 (38%), Gaps = 2/96 (2%)
Frame = -2
Query: 632 APVISAEKAYHEQLSVAEITNACFEPAXPDGEMRP--PSWQVHGLLYAVPVVTSYPRM*T 459
APV+ + ++ +V + A P P + P PS + VP S P+
Sbjct: 550 APVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPV 609
Query: 458 RPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 351
P P PSV + V P + SV P PE
Sbjct: 610 APVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 645
Score = 29.5 bits (63), Expect = 0.59
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -2
Query: 497 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 354
A PVV P + RP++P P S PV V + PP P
Sbjct: 639 AAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
Score = 27.9 bits (59), Expect = 1.8
Identities = 22/71 (30%), Positives = 28/71 (39%), Gaps = 2/71 (2%)
Frame = -2
Query: 557 PAXPDGEMRPPSWQVHGLLYA--VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSV 384
P P + P + VH A P V S P+ P +P PSV + V P + SV
Sbjct: 515 PQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSV 574
Query: 383 STTSHPPWCPE 351
P PE
Sbjct: 575 PQPPVAPVAPE 585
Score = 27.5 bits (58), Expect = 2.4
Identities = 25/100 (25%), Positives = 38/100 (38%), Gaps = 2/100 (2%)
Frame = -2
Query: 647 PLVTYAPVISAEKAYHEQLSVAEITNACFEPAXPDGEMRP--PSWQVHGLLYAVPVVTSY 474
P APV + ++ +V + A P P + P PS + VP S
Sbjct: 605 PQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSV 664
Query: 473 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 354
P+ P +P PSV + V P + ++ PP P
Sbjct: 665 PQPPAAPVVPEVPSVPQPPAVPVVPEAGQLNEPVVPPLPP 704
Score = 27.1 bits (57), Expect = 3.2
Identities = 21/71 (29%), Positives = 27/71 (38%), Gaps = 2/71 (2%)
Frame = -2
Query: 557 PAXPDGEMRP--PSWQVHGLLYAVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSV 384
P P + P PS + VP S P+ P +P PSV + V P + SV
Sbjct: 605 PQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSV 664
Query: 383 STTSHPPWCPE 351
P PE
Sbjct: 665 PQPPAAPVVPE 675
Score = 27.1 bits (57), Expect = 3.2
Identities = 18/49 (36%), Positives = 21/49 (42%)
Frame = -2
Query: 494 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 348
VP V S P+ P +P PSV + V P SV P PEA
Sbjct: 643 VPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEA 691
Score = 25.8 bits (54), Expect = 7.3
Identities = 31/119 (26%), Positives = 42/119 (35%), Gaps = 2/119 (1%)
Frame = -2
Query: 698 PHPSSRLTWCLTPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPAXPDGEMRPPSW 519
P P + P +H P APV + ++ + + A P P + P +
Sbjct: 515 PQPPAAPVVPEAPSVHQPPA--APVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEAL 572
Query: 518 QVHGLLYA--VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 348
V A P V S P+ P +P PSV V P SV P PEA
Sbjct: 573 SVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEA 631
Score = 25.4 bits (53), Expect = 9.6
Identities = 17/48 (35%), Positives = 20/48 (41%)
Frame = -2
Query: 491 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 348
P V S P+ P +P PSV + V P SV P PEA
Sbjct: 614 PEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEA 661
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -2
Query: 284 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 111
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 110 EPKSTK 93
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.5
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -2
Query: 452 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 366
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 5.5
Identities = 26/101 (25%), Positives = 40/101 (39%)
Frame = -2
Query: 491 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWPRFNVXXXXX 312
PVV + P T+P S P+ S + PVS + T + PP A R N
Sbjct: 988 PVVQNKPAA-TKPV--SMPAAKSKPAPMANPVSTAQQTQNRPP--APAMQARPNTTQAAA 1042
Query: 311 XXXXXXPKLGLALTTSSTSCTPSVLSCTGTSVRVWRRESSP 189
+ A T S++ PS ++ +S + S+P
Sbjct: 1043 PVTSTTTTIKQATTVSASKPAPSTVTSAASSPSNISKPSAP 1083
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 7.3
Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 4/36 (11%)
Frame = -3
Query: 616 PRRPTMNSFPSPR----SQTHASSPPXQMVKCDPRH 521
P RP + + P P+ S HA PP Q + P H
Sbjct: 1356 PVRPAVPTSPKPQIPDSSNVHAPPPPVQPMNAMPSH 1391
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 25.8 bits (54), Expect = 7.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 590 SVAEITNACFEPAXPDGEMRPPSWQV 513
SV ++T ACFEP+ ++ P W +
Sbjct: 800 SVTKVTCACFEPSLDYVVVKIPRWDL 825
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 25.8 bits (54), Expect = 7.3
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -3
Query: 703 VDLTRVPD*LGALPPVSTSHWSRTR-QSSLPRRPTMNSFPSP 581
+DLTR+P+ P SR ++L PT+NS PSP
Sbjct: 39 LDLTRIPE----FQPRRRIRTSRNHLDANLSNVPTINSIPSP 76
>SPAC3G9.14 |sak1||transcriptional repressor
Sak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 766
Score = 25.4 bits (53), Expect = 9.6
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = -1
Query: 684 QTNLVPYPPYPLPTGHVRASHLCREGLP 601
QTNL P + P H SHL + +P
Sbjct: 259 QTNLQLAPSFAAPQAHPLPSHLSQSNVP 286
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.4 bits (53), Expect = 9.6
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 628 QSSLPRRPTMNSFPSPRSQTHASSPP 551
+ SLPRRP+ +P S T ++ PP
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPP 763
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.6
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -2
Query: 455 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 360
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,127,487
Number of Sequences: 5004
Number of extensions: 67446
Number of successful extensions: 261
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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