Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_T7_B02
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 206 2e-54
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 206 2e-54
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 206 2e-54
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 70 3e-13
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 29 0.77
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 27 2.3
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 206 bits (504), Expect = 2e-54
Identities = 99/161 (61%), Positives = 116/161 (72%), Gaps = 1/161 (0%)
Frame = -2
Query: 799 PRXIXTEVKSVEMHHEALQEAVPGDNVGFXXXXXXXXXXXXXXCW*LQKX-PT*GAADFT 623
P + TEVKSVEMHHE+L +PGDNVGF K P G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 622 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAI 443
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACI 399
Query: 442 VNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVXQGCQ 320
+VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGV + +
Sbjct: 400 AKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVE 440
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 206 bits (504), Expect = 2e-54
Identities = 99/161 (61%), Positives = 116/161 (72%), Gaps = 1/161 (0%)
Frame = -2
Query: 799 PRXIXTEVKSVEMHHEALQEAVPGDNVGFXXXXXXXXXXXXXXCW*LQKX-PT*GAADFT 623
P + TEVKSVEMHHE+L +PGDNVGF K P G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 622 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAI 443
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACI 399
Query: 442 VNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVXQGCQ 320
+VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGV + +
Sbjct: 400 AKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVE 440
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 206 bits (504), Expect = 2e-54
Identities = 99/161 (61%), Positives = 116/161 (72%), Gaps = 1/161 (0%)
Frame = -2
Query: 799 PRXIXTEVKSVEMHHEALQEAVPGDNVGFXXXXXXXXXXXXXXCW*LQKX-PT*GAADFT 623
P + TEVKSVEMHHE+L +PGDNVGF K P G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 622 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAI 443
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACI 399
Query: 442 VNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVXQGCQ 320
+VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGV + +
Sbjct: 400 AKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVE 440
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 70.1 bits (164), Expect = 3e-13
Identities = 36/106 (33%), Positives = 56/106 (52%)
Frame = -2
Query: 655 KXPT*GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 476
K P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T + ++
Sbjct: 551 KNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKP 609
Query: 475 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG 338
P G I L P+C+E F+++ +GRF +RD TVAVG
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVG 655
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 29.1 bits (62), Expect = 0.77
Identities = 29/98 (29%), Positives = 42/98 (42%)
Frame = -2
Query: 628 FTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDA 449
F A++ + G I +G T VL H+ + K+ K + + S K
Sbjct: 498 FVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALV 551
Query: 448 AIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGV 335
I L PLC+ +E P LGRF +R TVA G+
Sbjct: 552 RISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGI 587
>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 27.5 bits (58), Expect = 2.3
Identities = 20/53 (37%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = -3
Query: 687 IASXYVAGDSKNXPPKVLQILQLKSLCLTILVKSQTVTHQSW--IATLPTLPA 535
+ S VA N PP VLQ+L + T K TH+SW P LPA
Sbjct: 189 VLSHKVAASPANFPPLVLQLLVVPVCDNTANAK----THKSWELFENTPQLPA 237
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 444 MAASPDLMDFGLTSVDLPVRRSTFS 518
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,090,065
Number of Sequences: 5004
Number of extensions: 61507
Number of successful extensions: 179
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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