Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_P18
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 341 1e-94
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 335 4e-93
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 174 2e-44
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 136 3e-33
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 29 0.84
SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo... 28 1.5
SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 27 4.5
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 341 bits (837), Expect = 1e-94
Identities = 152/224 (67%), Positives = 184/224 (82%)
Frame = +2
Query: 176 YCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTY 355
YCLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG Y
Sbjct: 24 YCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPY 83
Query: 356 RQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXX 535
R LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 84 RDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGG 143
Query: 536 XXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFM 715
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FM
Sbjct: 144 GTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFM 203
Query: 716 VDNEAIYDICRRNLDIERPTYTNLNXLIGQXVSSITASLXFDGA 847
VDNE+ YDICRRNLDIERP+Y NLN LI Q VSSITASL F+G+
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGS 247
Score = 49.2 bits (112), Expect = 7e-07
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWEL 174
MRE IS+HVGQAG QIGNACWEL
Sbjct: 1 MREIISIHVGQAGTQIGNACWEL 23
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 335 bits (824), Expect = 4e-93
Identities = 154/229 (67%), Positives = 185/229 (80%), Gaps = 5/229 (2%)
Frame = +2
Query: 176 YCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEV 340
YCLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP V+D+V
Sbjct: 24 YCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQV 82
Query: 341 RTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIF 520
RTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+F
Sbjct: 83 RTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVF 142
Query: 521 HXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHS 700
H LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++S
Sbjct: 143 HSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNS 202
Query: 701 DCAFMVDNEAIYDICRRNLDIERPTYTNLNXLIGQXVSSITASLXFDGA 847
DC FMVDNEA YDICRRNLDIERPTY NLN LI Q VSSITASL F G+
Sbjct: 203 DCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGS 251
Score = 51.2 bits (117), Expect = 2e-07
Identities = 22/23 (95%), Positives = 22/23 (95%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWEL 174
MRE ISVHVGQAGVQIGNACWEL
Sbjct: 1 MREVISVHVGQAGVQIGNACWEL 23
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 174 bits (423), Expect = 2e-44
Identities = 85/220 (38%), Positives = 125/220 (56%)
Frame = +2
Query: 185 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 364
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 365 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 544
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 545 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 724
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DN
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 725 EAIYDICRRNLDIERPTYTNLNXLIGQXVSSITASLXFDG 844
EA+ I L I+ P+Y +LN L+ ++ +T S F G
Sbjct: 205 EALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELL 177
MRE + + GQ G Q+G A W +
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTI 24
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 136 bits (330), Expect = 3e-33
Identities = 76/225 (33%), Positives = 122/225 (54%), Gaps = 3/225 (1%)
Frame = +2
Query: 179 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 358
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 359 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 532
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 533 XXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCA 709
L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSV 202
Query: 710 FMVDNEAIYDICRRNLDIERPTYTNLNXLIGQXVSSITASLXFDG 844
++DN A+ I L + PT+ N L+ +S+ T +L + G
Sbjct: 203 VVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 29.1 bits (62), Expect = 0.84
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = -2
Query: 838 EXQRSSDRGDXLSDETIQVGVGWALNVEITAADVIDG 728
+ Q+SSD + +D T VG+G AL I + D +DG
Sbjct: 182 DKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC1039.07c |||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 448
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/52 (30%), Positives = 23/52 (44%), Gaps = 1/52 (1%)
Frame = +2
Query: 89 KKLKSKCVSASLYTLAKPESRSVMPAGSFYCLE-HGIQPDGQMPTDKTIGGG 241
K LK KC + + + GS + E HGI PD + K++G G
Sbjct: 235 KALKKKCEERGMLLIIDEAQTGIGRTGSMFSFEHHGIVPD-ILTLSKSLGAG 285
>SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 990
Score = 26.6 bits (56), Expect = 4.5
Identities = 12/43 (27%), Positives = 18/43 (41%)
Frame = +1
Query: 151 IGNACWELLLPGARHPA*WPDAHRQDHRGWRRFFQHFLQRDRS 279
+ A W++LLP WP + R +RR L+ S
Sbjct: 412 VEKAWWKMLLPSPEQWNSWPSSSRDSFNSYRRDLGDLLESSYS 454
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 186 STASSLMARCPQTRPSGVETILSTLSSARPELAST 290
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 205 WPDAHRQDHRGWRRFFQHFLQR 270
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,369,793
Number of Sequences: 5004
Number of extensions: 69882
Number of successful extensions: 213
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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