Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_O14
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 241 7e-65
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 233 2e-62
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 1e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 110 3e-25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 241 bits (591), Expect = 7e-65
Identities = 106/159 (66%), Positives = 127/159 (79%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 286
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 287 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 466
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 467 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV 583
+IR++AD C+GLQGFL+FH LL+ERL++
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAM 159
Score = 122 bits (293), Expect = 9e-29
Identities = 51/68 (75%), Positives = 62/68 (91%)
Frame = +3
Query: 585 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDV 764
+Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLD+
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219
Query: 765 ERPTYTNL 788
ERP+Y NL
Sbjct: 220 ERPSYENL 227
Score = 29.5 bits (63), Expect = 0.64
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1
Query: 790 NRLIGQIVSSITXSXKXDGXXN 855
NRLI Q+VSSIT S + +G N
Sbjct: 228 NRLIAQVVSSITASLRFEGSLN 249
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 233 bits (570), Expect = 2e-62
Identities = 107/164 (65%), Positives = 129/164 (78%), Gaps = 5/164 (3%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 271
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 272 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 451
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 452 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV 583
D VL+RIR++AD C+GLQGFL+FH LL+ERL++
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNM 163
Score = 128 bits (310), Expect = 7e-31
Identities = 54/68 (79%), Positives = 63/68 (92%)
Frame = +3
Query: 585 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDV 764
+YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLD+
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223
Query: 765 ERPTYTNL 788
ERPTY NL
Sbjct: 224 ERPTYENL 231
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1
Query: 790 NRLIGQIVSSITXSXKXDGXXN 855
NRLI Q+VSSIT S + G N
Sbjct: 232 NRLIAQVVSSITASLRFAGSLN 253
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (300), Expect = 1e-29
Identities = 61/157 (38%), Positives = 86/157 (54%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 286
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 287 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 466
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 467 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERL 577
+R+ A+ C LQGF + H LL+ ++
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKI 155
Score = 61.7 bits (143), Expect = 1e-10
Identities = 27/68 (39%), Positives = 42/68 (61%)
Frame = +3
Query: 585 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDV 764
+Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L +
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217
Query: 765 ERPTYTNL 788
+ P+Y +L
Sbjct: 218 KSPSYDDL 225
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 110 bits (264), Expect = 3e-25
Identities = 58/159 (36%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
Frame = +2
Query: 110 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 289
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 290 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 463
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 464 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 580
D I + AD L+GF + H L+ERL+
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Score = 43.2 bits (97), Expect = 5e-05
Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +3
Query: 588 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDV 764
Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I L
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220
Query: 765 ERPTY 779
+ PT+
Sbjct: 221 QNPTF 225
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 186 STASSLMARCPQTRPSGVETILSTLSSARPELAST 290
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 205 WPDAHRQDHRGWRRFFQHFLQR 270
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,396,251
Number of Sequences: 5004
Number of extensions: 71144
Number of successful extensions: 210
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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