Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP02_FL5_O12
         (833 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   180   3e-46
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   170   2e-43
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    93   6e-20
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    88   2e-18
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    26   5.7  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.6  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  180 bits (437), Expect = 3e-46
 Identities = 78/106 (73%), Positives = 89/106 (83%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 423
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARG
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARG 106



 Score = 71.7 bits (168), Expect = 1e-13
 Identities = 28/38 (73%), Positives = 35/38 (92%)
 Frame = +2

Query: 422 GHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 535
           GHYT+GKE+VD V D+IR++AD C+GLQGFL+FHSFGG
Sbjct: 106 GHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGG 143


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  170 bits (413), Expect = 2e-43
 Identities = 79/111 (71%), Positives = 90/111 (81%), Gaps = 5/111 (4%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 423
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARG
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARG 110



 Score = 72.9 bits (171), Expect = 5e-14
 Identities = 28/38 (73%), Positives = 36/38 (94%)
 Frame = +2

Query: 422 GHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 535
           GHYT+GKE++D VL+RIR++AD C+GLQGFL+FHSFGG
Sbjct: 110 GHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGG 147


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 92.7 bits (220), Expect = 6e-20
 Identities = 45/110 (40%), Positives = 67/110 (60%), Gaps = 2/110 (1%)
 Frame = +1

Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGSLH 432
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G  H
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSH 110



 Score = 28.3 bits (60), Expect = 1.4
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +2

Query: 428 YTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 535
           Y+  + I + ++D I + AD    L+GF + HS  G
Sbjct: 108 YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAG 143


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 87.8 bits (208), Expect = 2e-18
 Identities = 44/113 (38%), Positives = 63/113 (55%)
 Frame = +1

Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGSLHHWKG 444
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+G  H+ +G
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG--HYTEG 109



 Score = 50.0 bits (114), Expect = 4e-07
 Identities = 21/38 (55%), Positives = 26/38 (68%)
 Frame = +2

Query: 422 GHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGG 535
           GHYT G E+ D VLD +R+ A+ C  LQGF + HS GG
Sbjct: 104 GHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGG 141


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 3/64 (4%)
 Frame = +1

Query: 379 LITGKEDAANNYARGSLHHWKGN--RRFGFGQNPQAR*PVYRSARIPDLPLL-RGEVPAL 549
           L+TG +D  +    G LHH K        +  +P  + P  RS R  D   L R  V ++
Sbjct: 133 LLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMSI 192

Query: 550 GSLP 561
            S P
Sbjct: 193 KSTP 196


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +2

Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,566
Number of Sequences: 5004
Number of extensions: 54542
Number of successful extensions: 155
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)