Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP02_FL5_O09
         (858 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   293   3e-80
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   285   4e-78
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   147   2e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   130   3e-31
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom...    27   4.5  
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S...    26   7.9  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  293 bits (718), Expect = 3e-80
 Identities = 130/203 (64%), Positives = 156/203 (76%)
 Frame = +2

Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTAKTIGGGDDSFNTFFSETGAGK 283
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M         D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 643
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQV T+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 644 VVEPYNSIXXXXXXXXXXXCAFM 712
           VVEPYNS+           C FM
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFM 203


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  285 bits (700), Expect = 4e-78
 Identities = 131/208 (62%), Positives = 158/208 (75%), Gaps = 5/208 (2%)
 Frame = +2

Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTA-----KTIGGGDDSFNTFFSE 268
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT      K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 269 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 448
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 449 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 628
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 629 QVPTAVVEPYNSIXXXXXXXXXXXCAFM 712
           QV T+VVEPYNS+           C FM
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFM 207


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  147 bits (357), Expect = 2e-36
 Identities = 72/187 (38%), Positives = 103/187 (55%)
 Frame = +2

Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTAKTIGGGDDSFNTFFSETGAGK 283
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHG--TSEAQHERLNVYFNEAAGGK 58

Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 643
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+    
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 644 VVEPYNS 664
           VVEPYN+
Sbjct: 179 VVEPYNA 185


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  130 bits (313), Expect = 3e-31
 Identities = 70/190 (36%), Positives = 108/190 (56%), Gaps = 3/190 (1%)
 Frame = +2

Query: 107 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTAKTIGGGDDSFNTFFSETGAGKH 286
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 287 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 460
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 461 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VP 637
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q V 
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 638 TAVVEPYNSI 667
             VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189


>SPBC32F12.02 |rec14||recombination protein
           Rec14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 302

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 14/29 (48%), Positives = 16/29 (55%)
 Frame = -3

Query: 277 SSGLAEESVERIVSTPDGLGCGHLAIRLD 191
           S G AE S+   + TP  LG  HLA  LD
Sbjct: 42  SIGDAEHSLVEEIDTPHKLGVHHLATSLD 70


>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 10/29 (34%), Positives = 16/29 (55%), Gaps = 1/29 (3%)
 Frame = +2

Query: 584 YGKKSKLEFAIYPAPQVPTAVVE-PYNSI 667
           Y  + K+E   YP  ++P  V + PYN +
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKL 138


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,100,546
Number of Sequences: 5004
Number of extensions: 62566
Number of successful extensions: 163
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)