Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_N22
(846 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 266 4e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 258 5e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 132 5e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 1e-27
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 28 1.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.7
SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosac... 26 7.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 266 bits (651), Expect = 4e-72
Identities = 116/174 (66%), Positives = 141/174 (81%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 286
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 287 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 466
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 467 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 628
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPA
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174
Score = 57.6 bits (133), Expect = 2e-09
Identities = 28/57 (49%), Positives = 34/57 (59%)
Frame = +1
Query: 622 PRASGSTAVVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAXXNLDIEPPNLXNLNR 792
P ST+VVEPYNS+LTTH TL+ +DC + NLDIE P+ NLNR
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNR 229
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 258 bits (633), Expect = 5e-70
Identities = 117/179 (65%), Positives = 143/179 (79%), Gaps = 5/179 (2%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 271
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 272 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 451
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 452 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 628
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPA
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178
Score = 60.1 bits (139), Expect = 4e-10
Identities = 29/57 (50%), Positives = 34/57 (59%)
Frame = +1
Query: 622 PRASGSTAVVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAXXNLDIEPPNLXNLNR 792
P ST+VVEPYNS+LTTH TL++SDC + NLDIE P NLNR
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNR 233
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 132 bits (320), Expect = 5e-32
Identities = 65/174 (37%), Positives = 94/174 (54%)
Frame = +2
Query: 107 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 286
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 287 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 466
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 467 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 628
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PA
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172
Score = 34.7 bits (76), Expect = 0.017
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +1
Query: 622 PRASGSTAVVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAXXN-LDIEPPNLXNLN 789
P S VVEPYN+ L+ H +E+SD ++ +++S N L I+ P+ +LN
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSD-ETFCIDNEALSSIFANTLKIKSPSYDDLN 226
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (283), Expect = 1e-27
Identities = 62/174 (35%), Positives = 98/174 (56%), Gaps = 2/174 (1%)
Frame = +2
Query: 110 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 289
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 290 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 463
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 464 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 625
D I + AD L+GF + H L+ERL+ Y KK ++++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173
>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +1
Query: 616 HLPRASGSTAVVEPYNSILTTHTTLEHSD 702
HLP ASGS+ PY + TT +D
Sbjct: 155 HLPNASGSSVYQPPYTPFRSVSTTSSSND 183
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 186 STASSLMARCPQTRPSGVETILSTLSSARPELAST 290
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 305
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +1
Query: 706 ASWSTMKPSMTSAXXNLDIEPPNLXNLNRSHRTDXLLXXCFFXN 837
AS+ T KP + A PPN N+ HR D ++ F N
Sbjct: 240 ASFLTNKPVSSKATYQ-GYAPPNRFNIRPGHRWDGIIRGNGFEN 282
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 205 WPDAHRQDHRGWRRFFQHFLQR 270
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,300,218
Number of Sequences: 5004
Number of extensions: 68581
Number of successful extensions: 229
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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