Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_N21
(867 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 292 4e-80
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 285 6e-78
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 150 3e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 131 1e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 292 bits (717), Expect = 4e-80
Identities = 128/188 (68%), Positives = 155/188 (82%)
Frame = +2
Query: 50 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 229
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 230 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 409
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 410 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 589
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 590 VVEPYNSI 613
VVEPYNS+
Sbjct: 181 VVEPYNSV 188
Score = 67.3 bits (157), Expect = 3e-12
Identities = 28/34 (82%), Positives = 31/34 (91%)
Frame = +3
Query: 642 SDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 743
+DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLI 231
Score = 49.6 bits (113), Expect = 6e-07
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = +1
Query: 766 TASLRXDGALNVDLTEFXTNLVPYPRI 846
TASLR +G+LNVDL EF TNLVPYPRI
Sbjct: 239 TASLRFEGSLNVDLAEFQTNLVPYPRI 265
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 285 bits (699), Expect = 6e-78
Identities = 129/193 (66%), Positives = 157/193 (81%), Gaps = 5/193 (2%)
Frame = +2
Query: 50 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 214
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 215 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 394
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 395 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 574
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 575 QVSTAVVEPYNSI 613
QVST+VVEPYNS+
Sbjct: 180 QVSTSVVEPYNSV 192
Score = 71.7 bits (168), Expect = 1e-13
Identities = 31/35 (88%), Positives = 32/35 (91%)
Frame = +3
Query: 639 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 743
+SDC FMVDNEA YDICRRNLDIERPTY NLNRLI
Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235
Score = 48.0 bits (109), Expect = 2e-06
Identities = 22/27 (81%), Positives = 23/27 (85%)
Frame = +1
Query: 766 TASLRXDGALNVDLTEFXTNLVPYPRI 846
TASLR G+LNVDL EF TNLVPYPRI
Sbjct: 243 TASLRFAGSLNVDLNEFQTNLVPYPRI 269
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 150 bits (363), Expect = 3e-37
Identities = 73/187 (39%), Positives = 104/187 (55%)
Frame = +2
Query: 50 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 229
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 230 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 409
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 410 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 589
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 590 VVEPYNS 610
VVEPYN+
Sbjct: 179 VVEPYNA 185
Score = 34.7 bits (76), Expect = 0.017
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +3
Query: 639 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 743
+SD F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 195 NSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLV 229
Score = 32.3 bits (70), Expect = 0.092
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 766 TASLRXDGALNVDLTEFXTNLVPYPRI 846
T S R G LN DL + N+VP+PR+
Sbjct: 237 TTSFRFPGELNSDLRKLAVNMVPFPRL 263
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 131 bits (317), Expect = 1e-31
Identities = 71/190 (37%), Positives = 109/190 (57%), Gaps = 3/190 (1%)
Frame = +2
Query: 53 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 232
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 233 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 406
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 407 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 583
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 584 TAVVEPYNSI 613
VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +3
Query: 639 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGT 749
++D ++DN A+ I L + PT+ N+L+ T
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVST 234
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.0
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 129 STASSLMARCPQTRPSGVETILSTLSSARPELAST 233
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 148 WPDAHRQDHRGWRRFFQHFLQR 213
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,488,757
Number of Sequences: 5004
Number of extensions: 70968
Number of successful extensions: 220
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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