Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_K23
(1048 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 226 5e-60
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 118 1e-27
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 115 1e-26
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 4e-23
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 5e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 2e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 60 5e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 37 0.004
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.15
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 5.8
SPAC1751.01c |gti1||gluconate transporter inducer Gti1|Schizosac... 26 7.7
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 7.7
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 7.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 226 bits (552), Expect = 5e-60
Identities = 102/111 (91%), Positives = 105/111 (94%)
Frame = +3
Query: 195 PRIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFY 374
P IVGRPRH G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFY
Sbjct: 32 PSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFY 91
Query: 375 NELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSL 527
NELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSL
Sbjct: 92 NELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSL 142
Score = 70.1 bits (164), Expect = 5e-13
Identities = 30/36 (83%), Positives = 34/36 (94%)
Frame = +2
Query: 104 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 211
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPS G+
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR 37
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 118 bits (284), Expect = 1e-27
Identities = 55/118 (46%), Positives = 79/118 (66%), Gaps = 1/118 (0%)
Frame = +3
Query: 195 PRIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFY 374
P VGR +H+ VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + +
Sbjct: 36 PTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYS 95
Query: 375 N-ELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSL*LATRS 545
+ +L PEEHP+LLTE PL N+EK+ + +ET N PA+ ++Q VL+L + R+
Sbjct: 96 DQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASART 153
Score = 44.8 bits (101), Expect = 2e-05
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +2
Query: 122 LVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 211
+ +DNGSG KAGFAGDD P+ +FP+ G+
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 115 bits (276), Expect = 1e-26
Identities = 54/113 (47%), Positives = 79/113 (69%), Gaps = 2/113 (1%)
Frame = +3
Query: 195 PRIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHT 368
P IVGRP R + + KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +T
Sbjct: 30 PSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYT 89
Query: 369 FYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSL 527
F+ +L++ P +LLTE P+NP ANREKM + MFE + +YVAIQAVLSL
Sbjct: 90 FFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSL 142
Score = 39.1 bits (87), Expect = 0.001
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = +2
Query: 110 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 211
E A +V+DNG+G K G+A D+ PR FPS G+
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGR 35
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 103 bits (247), Expect = 4e-23
Identities = 45/93 (48%), Positives = 61/93 (65%), Gaps = 1/93 (1%)
Frame = +3
Query: 252 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 428
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 429 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSL 527
LNP NRE +IMFE+FN +Y+A+QAVL+L
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLAL 165
Score = 38.3 bits (85), Expect = 0.002
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +2
Query: 122 LVVDNGSGMCKAGFAGDDAPRAVFPS 199
+++DNG+G K G+AG+DAP VFP+
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPT 33
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 63.3 bits (147), Expect = 5e-11
Identities = 29/73 (39%), Positives = 41/73 (56%)
Frame = +3
Query: 294 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 473
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 474 ETFNTPAMYVAIQ 512
E+ PA Y+A Q
Sbjct: 123 ESLRCPATYLAKQ 135
Score = 39.9 bits (89), Expect = 6e-04
Identities = 16/35 (45%), Positives = 25/35 (71%)
Frame = +2
Query: 107 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 211
+EV+A+V+D GS + GF+G+D P+ V PS G+
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGE 43
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 61.7 bits (143), Expect = 2e-10
Identities = 29/96 (30%), Positives = 55/96 (57%), Gaps = 1/96 (1%)
Frame = +3
Query: 240 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 416
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 417 TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLS 524
TE NP R MT+++FE +N P++ I + S
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFS 156
Score = 30.7 bits (66), Expect = 0.36
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2
Query: 122 LVVDNGSGMCKAGFAGDDAPRAVF 193
LV+DNGS +AG+ G+ P+ VF
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVF 50
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 60.1 bits (139), Expect = 5e-10
Identities = 31/104 (29%), Positives = 55/104 (52%), Gaps = 2/104 (1%)
Frame = +3
Query: 213 PRHQGVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 386
P + GV + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 387 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAV 518
V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAV 140
Score = 37.1 bits (82), Expect = 0.004
Identities = 16/43 (37%), Positives = 29/43 (67%), Gaps = 1/43 (2%)
Frame = +2
Query: 107 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRG-KAPPSGRD 232
EE+ +LV+D GS + G+AG+++P + PS G ++ +GR+
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYGVRSDVTGRN 50
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 37.1 bits (82), Expect = 0.004
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 4/77 (5%)
Frame = +3
Query: 309 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 476
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 477 TFNTPAMYVAIQAVLSL 527
P +A + ++ L
Sbjct: 172 ECQVPGFTIAYEPLMGL 188
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.15
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 369 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 512
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.6 bits (56), Expect = 5.8
Identities = 13/40 (32%), Positives = 16/40 (40%)
Frame = -2
Query: 255 SPSVPYRPSRPDGGAFPRXEGNTARGASSPAKPALHIPEP 136
+P VP P RP P R A AL +P+P
Sbjct: 538 APEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQP 577
>SPAC1751.01c |gti1||gluconate transporter inducer
Gti1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 26.2 bits (55), Expect = 7.7
Identities = 16/62 (25%), Positives = 27/62 (43%)
Frame = -2
Query: 285 FCSVPHLLHKSPSVPYRPSRPDGGAFPRXEGNTARGASSPAKPALHIPEPLSTTNAATSS 106
F S+P + K P+ P P + + A +S+P P P+ + ++TSS
Sbjct: 193 FSSLPLVSSKPTGTPFVPKSPPSSSPSTTNVSNASTSSAPINP--RAPQTRRESISSTSS 250
Query: 105 SH 100
H
Sbjct: 251 IH 252
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 7.7
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 411 LLTEAPLNPKANREKMTQIMFETFN 485
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 26.2 bits (55), Expect = 7.7
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -2
Query: 276 VPHLLHKSPSVPYRPSRPDGGA 211
+ L+ K+PS PY SRP A
Sbjct: 306 IDQLISKAPSYPYSSSRPSASA 327
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,398,416
Number of Sequences: 5004
Number of extensions: 51222
Number of successful extensions: 148
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 549226150
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -