Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_K02
(838 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 120 3e-28
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 80 4e-16
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 79 1e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 71 2e-13
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 28 1.4
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.3
SPBC83.15 |||ribosome biogenesis protein Nsa1|Schizosaccharomyce... 27 3.3
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 27 4.4
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.4
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.8
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 120 bits (289), Expect = 3e-28
Identities = 51/65 (78%), Positives = 59/65 (90%)
Frame = +1
Query: 298 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 477
DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EAE+
Sbjct: 67 DLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEA 126
Query: 478 CDCLQ 492
CD LQ
Sbjct: 127 CDALQ 131
Score = 116 bits (278), Expect = 5e-27
Identities = 49/68 (72%), Positives = 57/68 (83%)
Frame = +3
Query: 99 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 278
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 279 PRAILXRL 302
PRA+L L
Sbjct: 61 PRAVLVDL 68
Score = 105 bits (252), Expect = 8e-24
Identities = 48/67 (71%), Positives = 55/67 (82%)
Frame = +2
Query: 560 KIREEYPDRIMNTYSVVPSPKVSDTVXEPYNATLSVHQLXENTDETYCIDNEALYXICFR 739
KIREEYPDR+M T+SV P+PK SDTV EPYNATLS+HQL EN+DET+CIDNEAL I
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFAN 213
Query: 740 TLKLSXP 760
TLK+ P
Sbjct: 214 TLKIKSP 220
Score = 28.3 bits (60), Expect = 1.4
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 757 PTYGDLNHLXSLTMSGV 807
P+Y DLNHL S M+GV
Sbjct: 220 PSYDDLNHLVSAVMAGV 236
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 79.8 bits (188), Expect = 4e-16
Identities = 32/65 (49%), Positives = 47/65 (72%)
Frame = +1
Query: 298 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 477
DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ A++
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 478 CDCLQ 492
C LQ
Sbjct: 133 CSGLQ 137
Score = 64.9 bits (151), Expect = 1e-11
Identities = 28/67 (41%), Positives = 42/67 (62%)
Frame = +2
Query: 560 KIREEYPDRIMNTYSVVPSPKVSDTVXEPYNATLSVHQLXENTDETYCIDNEALYXICFR 739
++ EY + +SV P+P+VS +V EPYN+ L+ H +N+D T+ +DNEA Y IC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 740 TLKLSXP 760
L + P
Sbjct: 220 NLDIERP 226
Score = 51.2 bits (117), Expect = 2e-07
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
Frame = +3
Query: 99 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 260
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 261 SGGKYVPRAI 290
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 78.6 bits (185), Expect = 1e-15
Identities = 32/65 (49%), Positives = 45/65 (69%)
Frame = +1
Query: 298 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 477
DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ A++
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 478 CDCLQ 492
C LQ
Sbjct: 129 CSGLQ 133
Score = 60.1 bits (139), Expect = 4e-10
Identities = 26/67 (38%), Positives = 40/67 (59%)
Frame = +2
Query: 560 KIREEYPDRIMNTYSVVPSPKVSDTVXEPYNATLSVHQLXENTDETYCIDNEALYXICFR 739
++ EY + +SV P+P+VS +V EPYN+ L+ H + D T+ +DNE+ Y IC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 740 TLKLSXP 760
L + P
Sbjct: 216 NLDIERP 222
Score = 57.2 bits (132), Expect = 3e-09
Identities = 28/66 (42%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
Frame = +3
Query: 99 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 272
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 273 YVPRAI 290
YVPR+I
Sbjct: 61 YVPRSI 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 70.9 bits (166), Expect = 2e-13
Identities = 31/70 (44%), Positives = 47/70 (67%)
Frame = +3
Query: 102 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 281
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 282 RAILXRLGAR 311
RAIL L R
Sbjct: 63 RAILIDLEPR 72
Score = 55.2 bits (127), Expect = 1e-08
Identities = 22/67 (32%), Positives = 43/67 (64%), Gaps = 2/67 (2%)
Frame = +1
Query: 298 DLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKEA 471
DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +EA
Sbjct: 68 DLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREA 126
Query: 472 ESCDCLQ 492
+ D L+
Sbjct: 127 DGSDSLE 133
Score = 54.0 bits (124), Expect = 3e-08
Identities = 27/68 (39%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +2
Query: 560 KIREEYPDRIMNTYSVVP-SPKVSDTVXEPYNATLSVHQLXENTDETYCIDNEALYXICF 736
++ + YP +I+ TYSV P S VSD V +PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 737 RTLKLSXP 760
L P
Sbjct: 216 DRLHTQNP 223
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 28.3 bits (60), Expect = 1.4
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 403 AKGHYTEGAELVDSVLDVVRKEAESCDCLQXIP 501
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.3
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 138 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 239
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC83.15 |||ribosome biogenesis protein Nsa1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 27.1 bits (57), Expect = 3.3
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 767 PYVGGIVLACGSRYXREPRCRYSRFRLCFQXIDELKVWR 651
P + GI+ A G C YS C IDEL++WR
Sbjct: 137 PRIPGIIAAGGKE---NDVCLYSCNPTCKSNIDELELWR 172
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 26.6 bits (56), Expect = 4.4
Identities = 24/72 (33%), Positives = 31/72 (43%)
Frame = -3
Query: 392 PAPDCPKTKLSGRKICPKGPERTESMVPGSKSXEDGAGHVLAAGGFIVVYIDALQLQVRV 213
P+ PK L R I P GPE + + GS S A ++ +G + YID VR
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHSL--WAHYLWNSGIELANYIDKNPDTVRA 77
Query: 212 PMVGTGGVDAVL 177
V G A L
Sbjct: 78 KKVLELGAGAGL 89
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 334 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 435
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.8
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 476 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 339
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 355 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 486
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,028,588
Number of Sequences: 5004
Number of extensions: 56884
Number of successful extensions: 155
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -