Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_J22
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 289 3e-79
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 282 4e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 149 4e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 129 6e-31
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 29 1.1
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 26 5.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 289 bits (710), Expect = 3e-79
Identities = 127/186 (68%), Positives = 153/186 (82%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 646 VVEPYN 663
VVEPYN
Sbjct: 181 VVEPYN 186
Score = 86.2 bits (204), Expect = 5e-18
Identities = 39/56 (69%), Positives = 45/56 (80%)
Frame = +3
Query: 687 LEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRXIGXIVSSIXASLXFXGAXNV 854
L+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNR I +VSSI ASL F G+ NV
Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNV 250
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 282 bits (692), Expect = 4e-77
Identities = 128/191 (67%), Positives = 155/191 (81%), Gaps = 5/191 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 QVSTAVVEPYN 663
QVST+VVEPYN
Sbjct: 180 QVSTSVVEPYN 190
Score = 91.9 bits (218), Expect = 1e-19
Identities = 42/56 (75%), Positives = 46/56 (82%)
Frame = +3
Query: 687 LEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRXIGXIVSSIXASLXFXGAXNV 854
L++SDC FMVDNEA YDICRRNLDIERPTY NLNR I +VSSI ASL F G+ NV
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNV 254
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 149 bits (362), Expect = 4e-37
Identities = 73/186 (39%), Positives = 103/186 (55%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 646 VVEPYN 663
VVEPYN
Sbjct: 179 VVEPYN 184
Score = 44.8 bits (101), Expect = 2e-05
Identities = 19/55 (34%), Positives = 31/55 (56%)
Frame = +3
Query: 687 LEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRXIGXIVSSIXASLXFXGAXN 851
+E+SD F +DNEA+ I L I+ P+Y +LN + +++ + S F G N
Sbjct: 193 VENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELN 247
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 129 bits (311), Expect = 6e-31
Identities = 70/188 (37%), Positives = 107/188 (56%), Gaps = 3/188 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 639
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 640 TAVVEPYN 663
VV+PYN
Sbjct: 180 DVVVQPYN 187
Score = 31.1 bits (67), Expect = 0.21
Identities = 13/53 (24%), Positives = 27/53 (50%)
Frame = +3
Query: 693 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRXIGXIVSSIXASLXFXGAXN 851
++D ++DN A+ I L + PT+ N+ + ++S+ +L + G N
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMN 250
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = -3
Query: 836 EXQRSXDRGDNXSDXTIQVGVGWALNVEITAADVIDG 726
+ Q+S D ++ +D T VG+G AL I + D +DG
Sbjct: 182 DKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 26.2 bits (55), Expect = 5.9
Identities = 8/34 (23%), Positives = 17/34 (50%)
Frame = -3
Query: 767 ALNVEITAADVIDGFIVDHESTVRVLKXVWVVRI 666
+LN+ ++ G D+E+ ++ +WV I
Sbjct: 218 SLNINFMGVGIVSGLTADYETQEQITASIWVEHI 251
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,332,621
Number of Sequences: 5004
Number of extensions: 68433
Number of successful extensions: 191
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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