Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_H15
(867 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 256 4e-69
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 248 6e-67
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 127 2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 117 2e-27
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 29 0.86
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.0
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 26 8.0
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 26 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 256 bits (626), Expect = 4e-69
Identities = 117/189 (61%), Positives = 140/189 (74%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPGXSGFHX 643
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL P
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQ-VST 179
Query: 644 PSSXPYNSI 670
PYNS+
Sbjct: 180 SVVEPYNSV 188
Score = 53.6 bits (123), Expect = 3e-08
Identities = 28/58 (48%), Positives = 38/58 (65%), Gaps = 1/58 (1%)
Frame = +3
Query: 606 EFAIYPXPQVSTXR-RRXPTILSSPPTQPLXHSDCAFMVDNEAIYDIWRRNXXIEXPT 776
+F++YP PQVST ++L++ T L +DC FMVDNE+ YDI RRN IE P+
Sbjct: 168 QFSVYPAPQVSTSVVEPYNSVLTTHATLDL--ADCTFMVDNESCYDICRRNLDIERPS 223
Score = 31.9 bits (69), Expect = 0.12
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1
Query: 778 YTNLNXLIGXIXSSXTXSLKXXGXXNVD 861
Y NLN LI + SS T SL+ G NVD
Sbjct: 224 YENLNRLIAQVVSSITASLRFEGSLNVD 251
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 248 bits (608), Expect = 6e-67
Identities = 118/194 (60%), Positives = 142/194 (73%), Gaps = 5/194 (2%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 268
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 269 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 448
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 449 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPGX 628
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 629 SGFHXPSSXPYNSI 670
PYNS+
Sbjct: 180 Q-VSTSVVEPYNSV 192
Score = 60.5 bits (140), Expect = 3e-10
Identities = 32/60 (53%), Positives = 40/60 (66%), Gaps = 1/60 (1%)
Frame = +3
Query: 600 NWEFAIYPXPQVSTXR-RRXPTILSSPPTQPLXHSDCAFMVDNEAIYDIWRRNXXIEXPT 776
N +F++YP PQVST ++L++ T L +SDC FMVDNEA YDI RRN IE PT
Sbjct: 170 NLQFSVYPAPQVSTSVVEPYNSVLTTHAT--LDNSDCTFMVDNEACYDICRRNLDIERPT 227
Score = 31.9 bits (69), Expect = 0.12
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1
Query: 778 YTNLNXLIGXIXSSXTXSLKXXGXXNVD 861
Y NLN LI + SS T SL+ G NVD
Sbjct: 228 YENLNRLIAQVVSSITASLRFAGSLNVD 255
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 127 bits (306), Expect = 2e-30
Identities = 62/161 (38%), Positives = 88/161 (54%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 586
+R+ A+ C LQGF + H LL+ ++ +Y
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 117 bits (282), Expect = 2e-27
Identities = 66/190 (34%), Positives = 99/190 (52%), Gaps = 2/190 (1%)
Frame = +2
Query: 107 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 286
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 287 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 460
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 461 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPGXSGFH 640
D I + AD L+GF + H L+ERL+ Y KK P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 641 XPSSXPYNSI 670
PYNS+
Sbjct: 180 DVVVQPYNSL 189
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 29.1 bits (62), Expect = 0.86
Identities = 30/96 (31%), Positives = 38/96 (39%), Gaps = 4/96 (4%)
Frame = +3
Query: 462 TESASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWEFAIY-PXPQVS 638
T S+++ TS S SS SV P + PY S +T S + A + P Q S
Sbjct: 198 TTSSATHTS-QFSTSSSSSVNSVHTPVMVPNPYFQYNSSMTAPSSSSSSVAPFVPRRQFS 256
Query: 639 TXRRRXPT---ILSSPPTQPLXHSDCAFMVDNEAIY 737
P I SPP P AFM N+ Y
Sbjct: 257 VSSASDPPQTPISMSPPIPPTPSQFSAFMYQNQQSY 292
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.0
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 183 STASSLMARCPQTRPSGVETILSTLSSARPELAST 287
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 202 WPDAHRQDHRGWRRFFQHFLQR 267
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +3
Query: 621 PXPQVSTXRRRXPTILSSPPTQPLXHSDCAFMVDN 725
P P ++T R + SSPP QP S + M N
Sbjct: 1603 PKPAIATHSRYNASFSSSPPPQPGNSSGMSAMNTN 1637
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +3
Query: 621 PXPQVSTXRRRXPTILSSPPTQPLXHSDCAFMVDN 725
P P ++T R + SSPP QP S + M N
Sbjct: 1603 PKPAIATHSRYNASFSSSPPPQPGNSSGMSAMNTN 1637
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,261,596
Number of Sequences: 5004
Number of extensions: 65890
Number of successful extensions: 180
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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