Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_H01
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 163 2e-41
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 47 3e-06
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 41 3e-04
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 39 0.001
SPAC26H5.06 |pot1||telomere end-binding protein Pot1 |Schizosacc... 29 0.84
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 27 2.6
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 163 bits (397), Expect = 2e-41
Identities = 84/197 (42%), Positives = 115/197 (58%), Gaps = 4/197 (2%)
Frame = +1
Query: 16 RRFFLQEDPSLPKATVVKICETTEHRGQRICIRGWVHRLRRQGKSLAFLTLRDGTGYLQC 195
+ L+E P A + I ++T R R+ + GWVHR+R Q K + F+ LRDGTG+LQC
Sbjct: 115 KNIVLKEPKDAPAAKKIAIIDSTNFRDSRVRVNGWVHRMRTQ-KGIIFIILRDGTGFLQC 173
Query: 196 VLHGLLCQ--TYNALVLSTESSVVLYGKLEAVPEGKKAPGGHELTADYWELIGLAPPGGA 369
VL G + +Y+ + L ES+V LYG ++ +PEGK APG HEL DY++++ AP G
Sbjct: 174 VLSGKVYDRASYDFINLGPESTVCLYGVIKELPEGKSAPGNHELVVDYYQILHAAPTGEE 233
Query: 370 DAI--LNEEALPDVQLDNRHIMIRGENTSKVLRARAAVTRAFREHFASRRYXXXXXXXXX 543
LN EA P LD RH++IRGE S VL+ RA RA R+ F + +
Sbjct: 234 AFTNRLNAEAEPSYLLDQRHLVIRGETASSVLKVRARALRAMRDTFENLKMTEVTPPCMV 293
Query: 544 XXXCEGGSTLFKFXYFG 594
EGG+TLFKF Y+G
Sbjct: 294 QTQVEGGATLFKFNYYG 310
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2
Query: 593 GEXAYLTXXSXLYLXTCLATLG 658
G+ AYLT S LYL L LG
Sbjct: 310 GQDAYLTQSSQLYLEAALPALG 331
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 47.2 bits (107), Expect = 3e-06
Identities = 39/163 (23%), Positives = 69/163 (42%)
Frame = +1
Query: 88 HRGQRICIRGWVHRLRRQGKSLAFLTLRDGTGYLQCVLHGLLCQTYNALVLSTESSVVLY 267
H G+ I I GWV +R+ K++ F + DGT Q L ++ + LS +SV +
Sbjct: 18 HNGELISINGWVRSIRKL-KNVCFAMVSDGT--CQQALQ-VVTSPEQSKKLSYGASVNIE 73
Query: 268 GKLEAVPEGKKAPGGHELTADYWELIGLAPPGGADAILNEEALPDVQLDNRHIMIRGENT 447
G+L K +EL A+ ++ G I + ++ H+ +R
Sbjct: 74 GQLAVSKNAKLGLQQYELLAEKIKIYGQINDDNY-PIQKKHLTTEMLRQIPHLRLRTAKQ 132
Query: 448 SKVLRARAAVTRAFREHFASRRYXXXXXXXXXXXXCEGGSTLF 576
++ R R+ +A R+ F+S+ + CEG +F
Sbjct: 133 GEIFRLRSDSLKALRQFFSSKDFTETNPPIITSSDCEGAGEVF 175
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 40.7 bits (91), Expect = 3e-04
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = +1
Query: 400 DVQLDNRHIMIRGENTSKVLRARAAVTRAFREHFASRRYXXXXXXXXXXXXCEGGSTLFK 579
D +LDNR + +R + +A + +AFRE S + EGGS +FK
Sbjct: 235 DTRLDNRVLDLRTPTNQAIFDIQAGICQAFREFLLSNSFNEIHTPKMSGASSEGGSNVFK 294
Query: 580 FXYF 591
YF
Sbjct: 295 IQYF 298
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 38.7 bits (86), Expect = 0.001
Identities = 49/170 (28%), Positives = 73/170 (42%), Gaps = 29/170 (17%)
Frame = +1
Query: 94 GQRICIRGWVHRLRRQ-GKSLAFLTLRDGTGYLQCVLHG---LLCQTYNALVLST----- 246
G ++ I+GW+ RQ KS++F LRD G + +L +L Q LV ST
Sbjct: 15 GTKVSIQGWLVATSRQVSKSISFHQLRDTHGTILQLLSTDKIILQQKREPLVSSTDFSQQ 74
Query: 247 --------------ESSVVLYGKLEAVPEGKKAPGGH-ELTADYWELIGLAP-----PGG 366
ES V + GKL+ PE + PG EL + +L+ +A PG
Sbjct: 75 KSTSVMRTLSSIPPESVVQVTGKLQRRPEHDRRPGNEFELHVEDVKLLNVAKNLQLFPG- 133
Query: 367 ADAILNEEALPDVQLDNRHIMIRGENTSKVLRARAAVTRAFREHFASRRY 516
+E+ +QL NRHI +R + LR R+ + F R +
Sbjct: 134 -----DEKPGMRIQLANRHIQLRAPKYNSYLRQRSRLAYQVHSFFNDREF 178
>SPAC26H5.06 |pot1||telomere end-binding protein Pot1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 555
Score = 29.1 bits (62), Expect = 0.84
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 172 DGTGYLQCVLHGLLCQTYNALVLSTES 252
D GYL+C+LHG + YN + +S
Sbjct: 295 DHLGYLECILHGDSAKRYNMSIEKVDS 321
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 374 ASAPPGGASPINSQ*SAVSS*PPGAFFPSG 285
A+APPG N+ S+ + PPG P+G
Sbjct: 554 ATAPPGAPGSYNTSESSNLNAPPGVSMPNG 583
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,775,245
Number of Sequences: 5004
Number of extensions: 58350
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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