BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_G20
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 29 1.1
SPBC29A10.01 |ccr1|SPBC365.17|NADPH-cytochrome p450 reductase |S... 27 2.6
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 2.6
SPCC663.06c |||short chain dehydrogenase |Schizosaccharomyces po... 27 2.6
SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA synthase|Sc... 26 5.9
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 26 7.8
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/48 (37%), Positives = 32/48 (66%), Gaps = 3/48 (6%)
Frame = +3
Query: 318 ASRNEDLSPLHWRVLASNTGSIR--NNWTVGSNI-GQKNTHSLKGRSS 452
AS +E+L+P + LASNT ++ N+ + S+I G + T++L G+S+
Sbjct: 196 ASTSENLTPTSSKSLASNTSLVQSFNSASRSSSISGNQYTYNLLGKST 243
>SPBC29A10.01 |ccr1|SPBC365.17|NADPH-cytochrome p450 reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 678
Score = 27.5 bits (58), Expect = 2.6
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +2
Query: 374 GFNSKQLDSWLKYWAEEYPFA-ERQKFLNQYPHFKTNIQGLNIHFMRITPKVPKDVEIVP 550
G S+QL S++ +A E +K N Y +FK N+ L+++ ++ +V D
Sbjct: 366 GIVSRQLLSFIAPFAPTPESKQELEKLGNDYDYFKKNVVDLHLNLAQVLRRVSPDAPFTK 425
Query: 551 L 553
L
Sbjct: 426 L 426
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 27.5 bits (58), Expect = 2.6
Identities = 8/23 (34%), Positives = 12/23 (52%)
Frame = +2
Query: 395 DSWLKYWAEEYPFAERQKFLNQY 463
++W YW YPF + K +Y
Sbjct: 39 ENWKSYWKSHYPFVKNDKGKKKY 61
>SPCC663.06c |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 253
Score = 27.5 bits (58), Expect = 2.6
Identities = 23/88 (26%), Positives = 38/88 (43%), Gaps = 1/88 (1%)
Frame = +2
Query: 467 HFKTNIQGLNIHFMRITPKVPKDVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDR-NFA 643
H+KTN+ G IH + + K E ++ GS+ F+ SK NF
Sbjct: 110 HYKTNVLG-PIHVYQAFYPLVKKGESKIIVFTSSLVGSMGAFFPFNQSGYGQSKAALNFT 168
Query: 644 LEIIAPSLPGYGFSDXAVRPGLAAAEXA 727
++ I+ L GF ++ PG+ + A
Sbjct: 169 MKEISFELQDEGFIVISIHPGMVRTDSA 196
>SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA
synthase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 447
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -3
Query: 357 PSNGGAKGLRFLMRSLSSFTIVSLKVISKGLMLVS 253
PSN +G+R L+ +L + ++ K + KGLM ++
Sbjct: 273 PSNPELEGVRELLSTLDAKKSLTDKALEKGLMAIT 307
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = -2
Query: 499 NIQTLDISLEMRILVKELLPF-SEWVFFCPIFEPTVQ 392
N+QTLD+SL L+K+ + +++ + EPT+Q
Sbjct: 98 NLQTLDLSLAEFSLIKDAQNYLNKYASYFQAHEPTLQ 134
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,397,288
Number of Sequences: 5004
Number of extensions: 71091
Number of successful extensions: 204
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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