Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_G07
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 137 2e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 85 2e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 83 4e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 71 2e-13
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.4
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.5
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 5.9
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 26 5.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 137 bits (331), Expect = 2e-33
Identities = 62/95 (65%), Positives = 70/95 (73%)
Frame = +2
Query: 293 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 472
DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EAE+
Sbjct: 67 DLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEA 126
Query: 473 CDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPD 577
CD LQGFQ KIREEYPD
Sbjct: 127 CDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPD 161
Score = 116 bits (278), Expect = 6e-27
Identities = 49/68 (72%), Positives = 57/68 (83%)
Frame = +1
Query: 94 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 273
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 274 PRAILXRL 297
PRA+L L
Sbjct: 61 PRAVLVDL 68
Score = 58.4 bits (135), Expect = 1e-09
Identities = 29/61 (47%), Positives = 35/61 (57%)
Frame = +1
Query: 571 P*RIMNTYXXXXXXXXXXXXXXXYNATLSVHQLXENTDXTYCIDNEALYDICFRTLKLSX 750
P R+M T+ YNATLS+HQL EN+D T+CIDNEAL I TLK+
Sbjct: 160 PDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKS 219
Query: 751 P 753
P
Sbjct: 220 P 220
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 84.6 bits (200), Expect = 2e-17
Identities = 34/67 (50%), Positives = 49/67 (73%)
Frame = +2
Query: 293 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 472
DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ A++
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 473 CDCLQGF 493
C LQGF
Sbjct: 133 CSGLQGF 139
Score = 51.2 bits (117), Expect = 2e-07
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
Frame = +1
Query: 94 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 255
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 256 SGGKYVPRAI 285
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
Score = 47.2 bits (107), Expect = 3e-06
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 640 YNATLSVHQLXENTDXTYCIDNEALYDICFRTLKLSXP 753
YN+ L+ H +N+D T+ +DNEA YDIC R L + P
Sbjct: 189 YNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 83.4 bits (197), Expect = 4e-17
Identities = 34/67 (50%), Positives = 47/67 (70%)
Frame = +2
Query: 293 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAES 472
DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ A++
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 473 CDCLQGF 493
C LQGF
Sbjct: 129 CSGLQGF 135
Score = 57.2 bits (132), Expect = 3e-09
Identities = 28/66 (42%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
Frame = +1
Query: 94 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 267
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 268 YVPRAI 285
YVPR+I
Sbjct: 61 YVPRSI 66
Score = 41.9 bits (94), Expect = 1e-04
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +1
Query: 640 YNATLSVHQLXENTDXTYCIDNEALYDICFRTLKLSXP 753
YN+ L+ H + D T+ +DNE+ YDIC R L + P
Sbjct: 185 YNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 70.9 bits (166), Expect = 2e-13
Identities = 31/70 (44%), Positives = 47/70 (67%)
Frame = +1
Query: 97 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 276
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 277 RAILXRLGAR 306
RAIL L R
Sbjct: 63 RAILIDLEPR 72
Score = 60.5 bits (140), Expect = 3e-10
Identities = 26/96 (27%), Positives = 50/96 (52%), Gaps = 2/96 (2%)
Frame = +2
Query: 293 DLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKEA 466
DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +EA
Sbjct: 68 DLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREA 126
Query: 467 ESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYP 574
+ D L+GF ++ + YP
Sbjct: 127 DGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYP 162
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 133 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 234
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 329 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 430
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 398 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 487
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 471 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 334
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 26.2 bits (55), Expect = 5.9
Identities = 23/72 (31%), Positives = 31/72 (43%)
Frame = -3
Query: 387 PAPDCPKTKLSGRKICPKGPERTESMVPGSKSXEDGAGHVLAAGGFIVVYVDALQLQVRV 208
P+ PK L R I P GPE + + GS S A ++ +G + Y+D VR
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHSL--WAHYLWNSGIELANYIDKNPDTVRA 77
Query: 207 PMVGTGGVDAVL 172
V G A L
Sbjct: 78 KKVLELGAGAGL 89
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 350 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 481
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,843,605
Number of Sequences: 5004
Number of extensions: 49544
Number of successful extensions: 137
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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