Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_G01
(859 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 215 9e-57
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 215 9e-57
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 152 7e-38
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 91 2e-19
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 77 2e-15
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 76 7e-15
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 74 3e-14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 41 2e-04
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 35 0.013
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 35 0.013
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 35 0.017
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.48
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 2.0
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 28 2.0
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 27 2.6
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.6
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 2.6
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 27 4.5
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 215 bits (524), Expect = 9e-57
Identities = 108/173 (62%), Positives = 130/173 (75%)
Frame = +2
Query: 62 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 241
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 242 KDEQDRCITIKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVT 421
DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSPGHVDFSSEVT
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGHVDFSSEVT 116
Query: 422 AALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDRALLXLXL 580
AALRVTDGAL QTETVLRQA+ ERI+P++ +NK+DRALL L +
Sbjct: 117 AALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQI 169
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 215 bits (524), Expect = 9e-57
Identities = 108/173 (62%), Positives = 130/173 (75%)
Frame = +2
Query: 62 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 241
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 242 KDEQDRCITIKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVT 421
DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSPGHVDFSSEVT
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGHVDFSSEVT 116
Query: 422 AALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDRALLXLXL 580
AALRVTDGAL QTETVLRQA+ ERI+P++ +NK+DRALL L +
Sbjct: 117 AALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQI 169
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 152 bits (368), Expect = 7e-38
Identities = 83/167 (49%), Positives = 112/167 (67%)
Frame = +2
Query: 80 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 259
+++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R
Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66
Query: 260 CITIKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 439
IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+
Sbjct: 67 GITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLC 118
Query: 440 DGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDRALLXLXL 580
DGA QT TVLRQA +RIK IL +NKMDR + L L
Sbjct: 119 DGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDRLITELKL 165
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 91.1 bits (216), Expect = 2e-19
Identities = 62/171 (36%), Positives = 88/171 (51%), Gaps = 7/171 (4%)
Frame = +2
Query: 68 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF-- 229
N + E DKKR IRN+ + AH+D GK+T T+ ++ G I G+
Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100
Query: 230 -TDTRKDEQDRCITIKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDF 406
D + E+++ ITI+S A +E + + N Q+ EK + IN+ID+PGH+DF
Sbjct: 101 KMDFMELEREKGITIQSAATHCTWERTVDQIEA--NEKQKTDFEKSYNINIIDTPGHIDF 158
Query: 407 SSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 559
+ EV ALRV DGA+ QT TV RQ + I F+NKMDR
Sbjct: 159 TIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 77.4 bits (182), Expect = 2e-15
Identities = 55/151 (36%), Positives = 78/151 (51%), Gaps = 2/151 (1%)
Frame = +2
Query: 113 NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAI 286
+IRN+ +IAH+D GK+TLT+ ++ G + +T T D E+ R ITI S AI
Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86
Query: 287 SMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 466
S + +QR INLID+PGH DF+ EV ++ V DGA+
Sbjct: 87 SFTWR------------NQR--------INLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 467 XXXXXXQTETVLRQAIAERIKPILFMNKMDR 559
QT+ V +QA I ++F+NKMDR
Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 75.8 bits (178), Expect = 7e-15
Identities = 49/157 (31%), Positives = 79/157 (50%)
Frame = +2
Query: 86 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 265
+RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I
Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106
Query: 266 TIKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 445
T+K+ SM + + +L+NLID+PGHVDF +EV +L +G
Sbjct: 107 TVKAQTCSMIYYYH----------------GQSYLLNLIDTPGHVDFRAEVMHSLAACEG 150
Query: 446 ALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMD 556
+ QT + A ++ + I +NK+D
Sbjct: 151 CILLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVD 187
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 73.7 bits (173), Expect = 3e-14
Identities = 51/164 (31%), Positives = 81/164 (49%), Gaps = 1/164 (0%)
Frame = +2
Query: 92 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 268
G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++
Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191
Query: 269 IKSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 448
IKST +++ VS D + K+ F ID+PGHVDF EV A + ++DG
Sbjct: 192 IKSTPLTL--------AVS----DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDGV 236
Query: 449 LXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDRALLXLXL 580
+ T +++ AI + +L +NK+DR +L L L
Sbjct: 237 VLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDRLILELRL 280
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 41.1 bits (92), Expect = 2e-04
Identities = 39/152 (25%), Positives = 61/152 (40%), Gaps = 1/152 (0%)
Frame = +2
Query: 104 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 283
KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S
Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108
Query: 284 ISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXX 463
+ +E + +D PGH D+ + DGA+
Sbjct: 109 VE--YETANRHYAH------------------VDCPGHADYIKNMITGAATMDGAIIVVS 148
Query: 464 XXXXXXXQTETVLRQAIAERIKPI-LFMNKMD 556
QT L A +K I +++NK+D
Sbjct: 149 ATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 35.1 bits (77), Expect = 0.013
Identities = 33/120 (27%), Positives = 53/120 (44%), Gaps = 2/120 (1%)
Frame = +2
Query: 98 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII--AGARAGETRFTDTRKDEQDRCITI 271
M K++ N+ VI HVD GKST T L+ K G I E T+ K +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKG------SF 54
Query: 272 KSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 451
K + + E + ++I + ++ K + + +ID+PGH DF + D A+
Sbjct: 55 KYAWVLDKLKAERERGITIDIALWKFETPK-YNVTVIDAPGHRDFIKNMITGTSQADCAI 113
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 35.1 bits (77), Expect = 0.013
Identities = 33/120 (27%), Positives = 53/120 (44%), Gaps = 2/120 (1%)
Frame = +2
Query: 98 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII--AGARAGETRFTDTRKDEQDRCITI 271
M K++ N+ VI HVD GKST T L+ K G I E T+ K +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKG------SF 54
Query: 272 KSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 451
K + + E + ++I + ++ K + + +ID+PGH DF + D A+
Sbjct: 55 KYAWVLDKLKAERERGITIDIALWKFETPK-YNVTVIDAPGHRDFIKNMITGTSQADCAI 113
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 34.7 bits (76), Expect = 0.017
Identities = 33/120 (27%), Positives = 53/120 (44%), Gaps = 2/120 (1%)
Frame = +2
Query: 98 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII--AGARAGETRFTDTRKDEQDRCITI 271
M K++ N+ VI HVD GKST T L+ K G I E T+ K +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKG------SF 54
Query: 272 KSTAISMFFELEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 451
K + + E + ++I + ++ K + + +ID+PGH DF + D A+
Sbjct: 55 KYAWVLDKLKAERERGITIDIALWKFETPK-YNVTVIDAPGHRDFIKNMITGTSQADCAV 113
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.9 bits (64), Expect = 0.48
Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
Frame = +2
Query: 125 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 292
++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M
Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231
Query: 293 FFELEEK 313
FE K
Sbjct: 232 AFEAMRK 238
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 2.0
Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 1/111 (0%)
Frame = +2
Query: 122 NMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFE 301
N+ I HVD GKSTL +++ G++ R E + E + S A+ E
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV-DKRTMEK--IEREAKEAGKESWYLSWALDSTSE 296
Query: 302 LEEKDLVSITNPDQREKSEKGFLINLIDSPGHVDF-SSEVTAALRVTDGAL 451
EK E + F +L+D+PGH + ++ + A + G L
Sbjct: 297 EREKGKTVEVGRAYFETEHRRF--SLLDAPGHKGYVTNMINGASQADIGVL 345
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.9 bits (59), Expect = 2.0
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = -2
Query: 411 EEKSTCPGESIKLIKKPFSLFSRWSGFVMDTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 235
EE + P + K KK S F+ +T +S S++NI+ A+DL+ + S S ++
Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 27.5 bits (58), Expect = 2.6
Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 1/75 (1%)
Frame = -2
Query: 498 TVSVCTHTPD-TQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMD 322
T + C+ P+ T ST + +V+ S + ++ ST P ++ + S S V
Sbjct: 575 TTTTCSSRPEETISTVSTTSTVSESGSSSASITSTYPSSTLSMTTSHLS-----SSSVHS 629
Query: 321 TKSFSSSSKNIEMAV 277
+ + SSSS++ M++
Sbjct: 630 SSAHSSSSRSSSMSL 644
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/70 (28%), Positives = 33/70 (47%)
Frame = -2
Query: 501 STVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMD 322
S+ S T TP + STT+ + S + S ++S S+ + + S S S D
Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSS-SASSSGSISSAD 198
Query: 321 TKSFSSSSKN 292
K+ S+SS +
Sbjct: 199 AKTVSASSNS 208
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 27.5 bits (58), Expect = 2.6
Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = -2
Query: 501 STVSVCTHTPDTQSTT-TRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 325
+T S + +P + STT T +PS + S++ +S S+ S S S S
Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194
Query: 324 DTKSFSSSSKNIEMAV 277
+ S SSSS + + +
Sbjct: 195 SSSSSSSSSSSSSVPI 210
Score = 26.6 bits (56), Expect = 4.5
Identities = 19/71 (26%), Positives = 32/71 (45%)
Frame = -2
Query: 504 RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 325
++TVS + + T S+++ +PS + + TS S+ S S S S
Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183
Query: 324 DTKSFSSSSKN 292
+ S SSSS +
Sbjct: 184 SSSSSSSSSSS 194
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 26.6 bits (56), Expect = 4.5
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = +3
Query: 459 LTVCLVCVYKLKQYCVRLLPSASSLFCS*TKWTVLF 566
L V + YKL+ RL +A S+ C WT LF
Sbjct: 348 LNVIGIAAYKLEDPVHRLFVTAFSVCCECLAWTSLF 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,552,286
Number of Sequences: 5004
Number of extensions: 47643
Number of successful extensions: 186
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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