Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP02_FL5_E22
         (838 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   320   2e-88
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   315   6e-87
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   163   4e-41
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   137   2e-33
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S...    30   0.47 
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.6  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  320 bits (785), Expect = 2e-88
 Identities = 149/247 (60%), Positives = 183/247 (74%), Gaps = 1/247 (0%)
 Frame = +1

Query: 100 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 279
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 280 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 459
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 460 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 639
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQV T+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 640 VVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAA*SRH-*APNLHXLNXLIGQIVSSITX 816
           VVEPYNS+LTTH TL+ +DC      +        +     P+   LN LI Q+VSSIT 
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 817 SLXXXGA 837
           SL   G+
Sbjct: 241 SLRFEGS 247


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  315 bits (773), Expect = 6e-87
 Identities = 151/252 (59%), Positives = 185/252 (73%), Gaps = 6/252 (2%)
 Frame = +1

Query: 100 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 264
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 265 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 444
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 445 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 624
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 625 QVPTAVVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAA*SRH-*APNLHXLNXLIGQIV 801
           QV T+VVEPYNS+LTTH TL++SDC      +        +     P    LN LI Q+V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 802 SSITXSLXXXGA 837
           SSIT SL   G+
Sbjct: 240 SSITASLRFAGS 251


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  163 bits (395), Expect = 4e-41
 Identities = 85/248 (34%), Positives = 129/248 (52%), Gaps = 3/248 (1%)
 Frame = +1

Query: 100 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 279
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 280 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 459
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 460 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 639
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+    
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 640 VVEPYNSILTTHTTLEHSD---CASWSTMKPSMTSAA*SRH*APNLHXLNXLIGQIVSSI 810
           VVEPYN+ L+ H  +E+SD   C     +     +    +  +P+   LN L+  +++ +
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIK--SPSYDDLNHLVSAVMAGV 236

Query: 811 TXSLXXXG 834
           T S    G
Sbjct: 237 TTSFRFPG 244


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  137 bits (331), Expect = 2e-33
 Identities = 83/248 (33%), Positives = 128/248 (51%), Gaps = 4/248 (1%)
 Frame = +1

Query: 103 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 282
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 283 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 456
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 457 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VP 633
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q V 
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 634 TAVVEPYNSILTTHTTLEHSDCASWSTMKPSMTSAA*SRH*A-PNLHXLNXLIGQIVSSI 810
             VV+PYNS+L       ++D             AA   H   P  H  N L+  ++S+ 
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239

Query: 811 TXSLXXXG 834
           T +L   G
Sbjct: 240 TTTLRYPG 247


>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 29.9 bits (64), Expect = 0.47
 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = +1

Query: 580 YGKKSKLEFAIYPAPQVPTAVVE-PYNSILTTHTTLE 687
           Y  + K+E   YP  ++P  V + PYN +L   ++LE
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLE 146


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +2

Query: 179 STASSLMARCPQTRPSGVETILSTLSSARPELAST 283
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 198 WPDAHRQDHRGWRRFFQHFLQR 263
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,269,683
Number of Sequences: 5004
Number of extensions: 67096
Number of successful extensions: 182
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)