Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_E04
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 273 2e-74
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 253 3e-68
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 31 0.27
SPBC1703.03c |||armadillo repeat protein, unknown biological rol... 30 0.36
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.4
SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 27 2.5
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 7.7
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 273 bits (669), Expect = 2e-74
Identities = 136/237 (57%), Positives = 170/237 (71%), Gaps = 1/237 (0%)
Frame = +3
Query: 138 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGK 317
M I+ + ARQI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GK
Sbjct: 1 MAIQKVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGK 60
Query: 318 GVLTAIKDINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXX 497
GVL A+ ++N +IAP + KANL+VT Q+ DE +LKLDGTENKSKLGANAILGVS+
Sbjct: 61 GVLKAVGNVNNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICR 120
Query: 498 XXXXXXNVPLYKHLADLAG-NNDIVLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFS 674
+PL+K++A+ G VLPVP+FNV+NGGSHAG LA QEFMI PTGA +FS
Sbjct: 121 AGAAQKKLPLWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGDLAFQEFMILPTGAPSFS 180
Query: 675 EAMRMGSEVYXHLKXIIKEKLGLDSTAVGDXXGFXPNIXNXXDAXYLIQDAXXKAGY 845
EAMR G+E Y LK I K++ G + VGD G P++ +A LI +A KAGY
Sbjct: 181 EAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQEALDLIVEAINKAGY 237
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 253 bits (619), Expect = 3e-68
Identities = 123/235 (52%), Positives = 163/235 (69%), Gaps = 1/235 (0%)
Frame = +3
Query: 144 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 323
I+ I +R I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV
Sbjct: 4 IQKIYSRSIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGV 63
Query: 324 LTAIKDINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXX 503
A+ ++N +I P L K+++++T QR IDE M+KLDGT +KSKLGAN+I+GVS+
Sbjct: 64 TIAVHNVNNIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAA 123
Query: 504 XXXXNVPLYKHLADLAGNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEA 680
+PLY+++ LAG+ +PVP+FNV+NGG HAG LA QEFMI P A TFSE
Sbjct: 124 AAFLKIPLYEYIGKLAGSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEG 183
Query: 681 MRMGSEVYXHLKXIIKEKLGLDSTAVGDXXGFXPNIXNXXDAXYLIQDAXXKAGY 845
+R GSEVY LK + K+K G + VGD G P++ +A L+ +A +AGY
Sbjct: 184 LRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDLTTAEEALDLVNEAIKEAGY 238
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 30.7 bits (66), Expect = 0.27
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -1
Query: 691 PILMASLKVDAPVGKIMNSCMASLFPACDPPLITLNAGTGRTMSL 557
PI+ S + + +G+ +N + LFP+CD LI G T+ L
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
>SPBC1703.03c |||armadillo repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 664
Score = 30.3 bits (65), Expect = 0.36
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +3
Query: 39 NQILIFLPNPACKVQFC*IRISLNLRKSSSVLKMVIKSIKARQIFDS 179
NQI ++ P+C V+ I I N+ +S V K + +K +FDS
Sbjct: 241 NQIYLYFSYPSCLVRVYSIGIIYNIYQSGFVNKKMESVVKGISLFDS 287
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.4
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 174 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKDINEL 353
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 354 IAPELTKANLEV 389
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 527
Score = 27.5 bits (58), Expect = 2.5
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 482 PSCC*GWCCQEE 517
PSCC G CC+EE
Sbjct: 490 PSCCGGHCCKEE 501
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 7.7
Identities = 22/98 (22%), Positives = 42/98 (42%)
Frame = +3
Query: 120 SSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIK 299
+S V VI++ + G P + ++ LG F + S S + ++ + +
Sbjct: 1088 TSEVTLSVIRARPGLLVKMVDGEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLA 1147
Query: 300 SEYHGKGVLTAIKDINELIAPELTKANLEVTQQREIDE 413
+E H + K +NE +A L + N E T E+ +
Sbjct: 1148 TEIHYAASVGQNKYLNEYVA-SLIRTN-EKTHSTELSQ 1183
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,230,825
Number of Sequences: 5004
Number of extensions: 60891
Number of successful extensions: 143
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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