Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_D13
(885 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 332 3e-92
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 328 5e-91
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 164 1e-41
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 134 2e-32
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.2
SPBC4B4.06 |vps25||ESCRT II complex subunit Vps25|Schizosaccharo... 26 8.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 332 bits (817), Expect = 3e-92
Identities = 149/226 (65%), Positives = 180/226 (79%), Gaps = 1/226 (0%)
Frame = +3
Query: 129 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 308
HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++
Sbjct: 8 HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67
Query: 309 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 488
VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 489 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 668
C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNS 187
Query: 669 ILTTHTTLEHSDCAFMVDNEAIYDICRR-ISTLSANLTNLXRLIGQ 803
+LTTH TL+ +DC FMVDNE+ YDICRR + + NL RLI Q
Sbjct: 188 VLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQ 233
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 328 bits (807), Expect = 5e-91
Identities = 151/231 (65%), Positives = 182/231 (78%), Gaps = 6/231 (2%)
Frame = +3
Query: 129 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 293
HVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK V
Sbjct: 8 HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66
Query: 294 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 473
PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 474 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 653
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186
Query: 654 EPYNSILTTHTTLEHSDCAFMVDNEAIYDICRR-ISTLSANLTNLXRLIGQ 803
EPYNS+LTTH TL++SDC FMVDNEA YDICRR + NL RLI Q
Sbjct: 187 EPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQ 237
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 164 bits (399), Expect = 1e-41
Identities = 81/203 (39%), Positives = 115/203 (56%)
Frame = +3
Query: 135 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 314
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 315 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 494
LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 495 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIL 674
LQGF + H LL+ ++ +Y + F++ PAP+ S VVEPYN+ L
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
Query: 675 TTHTTLEHSDCAFMVDNEAIYDI 743
+ H +E+SD F +DNEA+ I
Sbjct: 188 SMHQLVENSDETFCIDNEALSSI 210
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 134 bits (324), Expect = 2e-32
Identities = 74/206 (35%), Positives = 115/206 (55%), Gaps = 3/206 (1%)
Frame = +3
Query: 135 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 314
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 315 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 488
LEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 489 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYN 665
L+GF + H L+ERL+ Y KK ++++P Q VS VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
Query: 666 SILTTHTTLEHSDCAFMVDNEAIYDI 743
S+L ++D ++DN A+ I
Sbjct: 188 SLLALKRLTLNADSVVVLDNAALAHI 213
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 187 STASSLMARCPQTRPSGVETILSTLSSARPELAST 291
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPBC4B4.06 |vps25||ESCRT II complex subunit
Vps25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 175
Score = 25.8 bits (54), Expect = 8.2
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -1
Query: 780 WLGWRSMSRYGGRCHRWL 727
W+ WRS+S +G +WL
Sbjct: 97 WVYWRSISEWGNMILKWL 114
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 206 WPDAHRQDHRGWRRFFQHFLQR 271
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,344,558
Number of Sequences: 5004
Number of extensions: 66728
Number of successful extensions: 193
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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