Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_B10
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 264 8e-72
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 138 7e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 136 5e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 3e-32
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 29 1.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 4.4
SPAC23C4.07 |tht2|mug22|meiotically upregulated gene Mug22|Schiz... 27 4.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 264 bits (648), Expect = 8e-72
Identities = 120/192 (62%), Positives = 142/192 (73%)
Frame = +3
Query: 81 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYV 260
MREIVH+QAGQCGNQ+G+ FW I+DEHG+D+ G+Y G S+ Q +R+NVY+NEA+ GKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 261 PRAVLVDLEPGTMDAVRSGPYGMLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVI 440
PRAVLVDLEPGTMDAV+SG +G LFRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDV+
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 441 RKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFXXXXXXXXXXXXX 620
R+EAE+CD LQGFQ KIREEYPDR+M TF
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 621 XXYNATLSVHQL 656
YNATLS+HQL
Sbjct: 181 EPYNATLSMHQL 192
Score = 64.9 bits (151), Expect = 1e-11
Identities = 30/51 (58%), Positives = 37/51 (72%)
Frame = +1
Query: 661 ENTDMSFCIDNEALYNICFKTLRLSSPSYGDLNXLISLTMSGVTTXPXVPG 813
EN+D +FCIDNEAL +I TL++ SPSY DLN L+S M+GVTT PG
Sbjct: 194 ENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 138 bits (335), Expect = 7e-34
Identities = 70/192 (36%), Positives = 97/192 (50%), Gaps = 2/192 (1%)
Frame = +3
Query: 81 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDR--INVYYNEASSGK 254
MREI+ + GQ G QIG+ WE+ EHG+ G E+ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 255 YVPRAVLVDLEPGTMDAVRSGPYGMLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 434
YVPR++ VDLEP +D VR+GPY LF P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 435 VIRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFXXXXXXXXXXX 614
IR+ A++C LQGF ++ EY + F
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 615 XXXXYNATLSVH 650
YN+ L+ H
Sbjct: 181 VVEPYNSVLTTH 192
Score = 45.6 bits (103), Expect = 9e-06
Identities = 23/76 (30%), Positives = 41/76 (53%)
Frame = +1
Query: 565 ES*IHSALYPRLR*AKSFWSPTTRRCPCTSWXENTDMSFCIDNEALYNICFKTLRLSSPS 744
+S + ++YP + + S P + + D +F +DNE+ Y+IC + L + PS
Sbjct: 164 KSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPS 223
Query: 745 YGDLNXLISLTMSGVT 792
Y +LN LI+ +S +T
Sbjct: 224 YENLNRLIAQVVSSIT 239
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 136 bits (328), Expect = 5e-33
Identities = 72/197 (36%), Positives = 101/197 (51%), Gaps = 7/197 (3%)
Frame = +3
Query: 81 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTG-------VYKGESDLQLDRINVYYNE 239
MRE++ V GQ G QIG+ WE+ EHG+ G V+K S L D +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLN-DGFGTFFSE 59
Query: 240 ASSGKYVPRAVLVDLEPGTMDAVRSGPYGMLFRPDNFVFGQSGAGNNWAKGHYTEGAELV 419
GK+VPR++ VDLEP +D VR+GPY LF P+ V G+ A NN+A+GHYT G E++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 420 DSVLDVIRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFXXXXXX 599
DSVL+ IR+ A++C LQGF ++ EY + F
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 600 XXXXXXXXXYNATLSVH 650
YN+ L+ H
Sbjct: 180 QVSTSVVEPYNSVLTTH 196
Score = 49.6 bits (113), Expect = 6e-07
Identities = 24/76 (31%), Positives = 43/76 (56%)
Frame = +1
Query: 565 ES*IHSALYPRLR*AKSFWSPTTRRCPCTSWXENTDMSFCIDNEALYNICFKTLRLSSPS 744
+S + ++YP + + S P + +N+D +F +DNEA Y+IC + L + P+
Sbjct: 168 KSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPT 227
Query: 745 YGDLNXLISLTMSGVT 792
Y +LN LI+ +S +T
Sbjct: 228 YENLNRLIAQVVSSIT 243
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (321), Expect = 3e-32
Identities = 59/168 (35%), Positives = 100/168 (59%), Gaps = 2/168 (1%)
Frame = +3
Query: 84 REIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYVP 263
REI+ +QAGQCGNQIGS+FW+ + EHG+ G + + +DR +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 264 RAVLVDLEPGTMDAVRSGPYGMLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 437
RA+L+DLEP ++ + S YG L+ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 438 IRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTF 581
I +EA+ D L+GF ++ + YP +I+ T+
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTY 169
Score = 35.1 bits (77), Expect = 0.013
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +1
Query: 664 NTDMSFCIDNEALYNICFKTLRLSSPSYGDLNXLISLTMSGVTTXPXVPG 813
N D +DN AL +I L +P++ N L+S MS TT PG
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 28.7 bits (61), Expect = 1.1
Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 315 GPYGMLFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 416
GPYG +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 384 AKGHYTEGAELVDSVLDVIRKEAESCDCLQ 473
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC23C4.07 |tht2|mug22|meiotically upregulated gene
Mug22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 26.6 bits (56), Expect = 4.4
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +1
Query: 661 ENTDMSFCIDNEALYNICFKTLRLSSPSYGDLNXLISLTMSGVTT 795
EN SF ID+ + K+L +PS+GD+ ++LT V++
Sbjct: 2 ENQVSSFLIDSPMEWKTYMKSLSDDNPSFGDVT--VNLTKISVSS 44
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,196,248
Number of Sequences: 5004
Number of extensions: 63018
Number of successful extensions: 158
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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