Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_B05
(922 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 124 6e-29
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 124 8e-29
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 117 1e-26
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 83 3e-16
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 67 2e-11
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 60 2e-09
At3g31540.1 68416.m04025 hypothetical protein 32 0.47
At5g10920.1 68418.m01267 argininosuccinate lyase, putative / arg... 31 0.81
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 1.1
At3g06570.1 68416.m00763 kelch repeat-containing F-box family pr... 30 1.9
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.9
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 2.5
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 29 3.3
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 5.7
At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421... 28 7.6
At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 28 7.6
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 124 bits (300), Expect = 6e-29
Identities = 54/86 (62%), Positives = 67/86 (77%)
Frame = +1
Query: 184 DDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 363
+DKG++TSED RFYA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK
Sbjct: 65 NDKGIQTSEDYRFYAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKK 124
Query: 364 MHGETPYEIMFGPDICGPGTKKVHVI 441
G+TPY IMFGPDICG TKKVH I
Sbjct: 125 FGGDTPYSIMFGPDICGYSTKKVHAI 150
Score = 107 bits (257), Expect = 1e-23
Identities = 44/70 (62%), Positives = 53/70 (75%)
Frame = +2
Query: 449 YKGKNHLIKKDIRCKDDVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIK 628
Y NHLIKKD+ C+ D TH+YT I +PD TY +LIDN + ++G L +DWD LPPKKIK
Sbjct: 153 YNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDNVEKQTGSLYSDWDLLPPKKIK 212
Query: 629 DPEAKKPEDW 658
DP AKKPEDW
Sbjct: 213 DPSAKKPEDW 222
Score = 34.3 bits (75), Expect = 0.12
Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 2/38 (5%)
Frame = +3
Query: 60 VFFEEKFPDDSWESNWVYSE--HPGKEFGKFKLTAGKF 167
V FEE+F DD WE+ WV SE G++K TAG +
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNW 60
Score = 29.5 bits (63), Expect = 3.3
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +2
Query: 614 PKKIKDPEAKKPEDW 658
PK+I D ++KKPEDW
Sbjct: 243 PKEIPDTDSKKPEDW 257
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 124 bits (299), Expect = 8e-29
Identities = 56/103 (54%), Positives = 71/103 (68%)
Frame = +1
Query: 184 DDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 363
+DKG++TSED RFYA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q
Sbjct: 65 NDKGIQTSEDYRFYAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTK 124
Query: 364 MHGETPYEIMFGPDICGPGTKKVHVIFXLQG*EPPHQKRYPLQ 492
G+TPY IMFGPDICG TKKVH I G +K P +
Sbjct: 125 FGGDTPYSIMFGPDICGYSTKKVHAILTYNGTNHLIKKEVPCE 167
Score = 105 bits (252), Expect = 4e-23
Identities = 42/70 (60%), Positives = 53/70 (75%)
Frame = +2
Query: 449 YKGKNHLIKKDIRCKDDVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIK 628
Y G NHLIKK++ C+ D TH+YT + +PD TY +LIDN + ++G L +DWD LP KKIK
Sbjct: 153 YNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTGSLYSDWDLLPAKKIK 212
Query: 629 DPEAKKPEDW 658
DP AKKPEDW
Sbjct: 213 DPSAKKPEDW 222
Score = 34.7 bits (76), Expect = 0.087
Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
Frame = +3
Query: 48 INCDVFFEEKFPDDSWESNWVYSE--HPGKEFGKFKLTAGKF 167
++ +V FEEKF +D WE WV S+ G++K TAG +
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNW 60
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 117 bits (281), Expect = 1e-26
Identities = 54/101 (53%), Positives = 71/101 (70%)
Frame = +1
Query: 175 DPEDDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLE 354
DP D+KG++T DA+ YA+S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ +
Sbjct: 69 DP-DNKGIQTYNDAKHYAISAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVN 127
Query: 355 QKDMHGETPYEIMFGPDICGPGTKKVHVIFXLQG*EPPHQK 477
QK G+TPY +MFGPDICG TKK+HVI QG P +K
Sbjct: 128 QKQFGGDTPYSLMFGPDICGTQTKKLHVIVSYQGQNYPIKK 168
Score = 95.1 bits (226), Expect = 6e-20
Identities = 38/70 (54%), Positives = 51/70 (72%)
Frame = +2
Query: 449 YKGKNHLIKKDIRCKDDVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIK 628
Y+G+N+ IKKD++C+ D H YT I +PD +Y VL+DN++ E G + DWD LPP+KIK
Sbjct: 159 YQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMYTDWDILPPRKIK 218
Query: 629 DPEAKKPEDW 658
AKKPEDW
Sbjct: 219 VKNAKKPEDW 228
Score = 33.1 bits (72), Expect = 0.27
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = +3
Query: 57 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFXRP--RG*QRFENL*RCEVL 224
++F EE F + W+S WV S+ E G FK TAGK+ P +G Q + + +
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPDNKGIQTYNDAKHYAIS 87
Query: 225 CSLP 236
+P
Sbjct: 88 AKIP 91
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 82.6 bits (195), Expect = 3e-16
Identities = 36/73 (49%), Positives = 52/73 (71%)
Frame = +1
Query: 175 DPEDDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLE 354
DP D+KG++T DA+ YA+S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ +
Sbjct: 69 DP-DNKGIQTYNDAKHYAISAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVN 127
Query: 355 QKDMHGETPYEIM 393
QK G+TPY ++
Sbjct: 128 QKQFGGDTPYSVL 140
Score = 60.1 bits (139), Expect = 2e-09
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = +2
Query: 536 DNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDW 658
D Y VL+DN++ E G + DWD LPP+KIK AKKPEDW
Sbjct: 134 DTPYSVLVDNKEREFGSMYTDWDILPPRKIKVKNAKKPEDW 174
Score = 33.1 bits (72), Expect = 0.27
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = +3
Query: 57 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFXRP--RG*QRFENL*RCEVL 224
++F EE F + W+S WV S+ E G FK TAGK+ P +G Q + + +
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPDNKGIQTYNDAKHYAIS 87
Query: 225 CSLP 236
+P
Sbjct: 88 AKIP 91
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 66.9 bits (156), Expect = 2e-11
Identities = 35/90 (38%), Positives = 53/90 (58%), Gaps = 4/90 (4%)
Frame = +1
Query: 184 DDKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKL 351
+D GL SE AR Y + ++ +P + + +V+Q+ V+ ++ ++CGG YLK +
Sbjct: 64 EDYGLLVSEKARKYGIVKELDEPLNLKEGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGW 123
Query: 352 EQKDMHGETPYEIMFGPDICGPGTKKVHVI 441
+ E+PY IMFGPD CG GT KVH I
Sbjct: 124 TPQGFDSESPYSIMFGPDKCG-GTNKVHFI 152
Score = 58.0 bits (134), Expect = 8e-09
Identities = 27/58 (46%), Positives = 36/58 (62%), Gaps = 4/58 (6%)
Frame = +2
Query: 497 DVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDF----LPPKKIKDPEAKKPEDW 658
D +H+YT I KPDN +L+D E+ + +L + DF +P K I DPE KKPEDW
Sbjct: 177 DKLSHVYTAILKPDNEVRILVDGEEKKKANLLSGEDFEPALIPAKTIPDPEDKKPEDW 234
Score = 32.3 bits (70), Expect = 0.47
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 796 IDNPXYKGVWAPKXIDXP 849
IDNP YKG+W P+ I P
Sbjct: 335 IDNPAYKGIWKPRDIPNP 352
Score = 31.9 bits (69), Expect = 0.61
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2
Query: 596 DWDFLPPKKIKDPEAKKPEDW 658
DWD P +I+D EA+KPE W
Sbjct: 250 DWDEDAPMEIEDEEAEKPEGW 270
Score = 31.1 bits (67), Expect = 1.1
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 575 ESGDLEADWDFLPPKKIKDPEAKKPEDW 658
E + W P+++ DPEA KPEDW
Sbjct: 262 EEAEKPEGWLDDEPEEVDDPEATKPEDW 289
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 60.5 bits (140), Expect = 2e-09
Identities = 35/93 (37%), Positives = 53/93 (56%), Gaps = 7/93 (7%)
Frame = +1
Query: 184 DDKGLKTSEDARFYALSRKF---KPFS-NEGKPLVVQFTVKHEQDIDCGGGYLKVF---D 342
DD GL SE A+ Y + ++ +P + NEG +V+Q+ + ++ ++CGG YLK +
Sbjct: 64 DDYGLLVSEKAKKYGIVKELDVDEPLNLNEGT-VVLQYEARFQEGLECGGAYLKYLRPQE 122
Query: 343 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVI 441
+ ++PY IMFGPD CG T KVH I
Sbjct: 123 AGWVPQGFDNDSPYSIMFGPDKCG-ATNKVHFI 154
Score = 58.8 bits (136), Expect = 5e-09
Identities = 27/58 (46%), Positives = 37/58 (63%), Gaps = 4/58 (6%)
Frame = +2
Query: 497 DVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDFLPP----KKIKDPEAKKPEDW 658
D+ +H+YT + K DN +L+D E+ + G+L + DF PP K I DPE KKPEDW
Sbjct: 179 DMLSHVYTAVLKSDNEVRILVDGEEKKKGNLLSAEDFEPPLIPSKTIPDPEDKKPEDW 236
Score = 32.7 bits (71), Expect = 0.35
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = +2
Query: 530 KPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDW 658
KPD+ E + E + W P +++DPEA KPEDW
Sbjct: 249 KPDDWDEDAPMEIEDEEAEKPEGWLDDEPVEVEDPEASKPEDW 291
Score = 32.3 bits (70), Expect = 0.47
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 796 IDNPXYKGVWAPKXIDXP 849
IDNP YKG+W P+ I P
Sbjct: 337 IDNPAYKGIWKPRDIPNP 354
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 32.3 bits (70), Expect = 0.47
Identities = 18/53 (33%), Positives = 24/53 (45%)
Frame = +2
Query: 458 KNHLIKKDIRCKDDVYTHLYTLIAKPDNTYEVLIDNEKVESGDLEADWDFLPP 616
KN I + CKDD YT + +A D E L+ + G L+ FL P
Sbjct: 252 KNMKIIEGFPCKDDAYTDYFFFVALEDAVLEDLVGKVLTKWGILDRPIRFLEP 304
>At5g10920.1 68418.m01267 argininosuccinate lyase, putative /
arginosuccinase, putative similar to argininosuccinate
lyase [Nostoc punctiforme] GI:7672743; contains Pfam
profile PF00206: Lyase
Length = 517
Score = 31.5 bits (68), Expect = 0.81
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Frame = +2
Query: 323 DTSRSLTAN-WSRRTCTERLHMRLCSALTSVVQEPRRYT*SSXYKGKNHLIKKDIR--CK 493
D R + AN + RT E +HM + +ALT ++ EP + ++ + +N + D R C+
Sbjct: 121 DIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTA--RSRNDQVATDFRLWCR 178
Query: 494 DDVYTHLYTLIAKPDNTYEVLID 562
D + T+I K N L++
Sbjct: 179 DAI----DTIIVKIRNLQRALVE 197
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 1.1
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +1
Query: 277 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 390
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At3g06570.1 68416.m00763 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 390
Score = 30.3 bits (65), Expect = 1.9
Identities = 32/120 (26%), Positives = 48/120 (40%)
Frame = +2
Query: 221 SMLSPVSSNRSVMRVNPWSSSSLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRLCSA 400
SMLSP R + V P S S+ ++ ++ D S+ A R R +C +
Sbjct: 3 SMLSPPVKKRKI--VAPQSQSASASFQ---SLPDDLILSIVARVPR--LYHRTVSLVCKS 55
Query: 401 LTSVVQEPRRYT*SSXYKGKNHLIKKDIRCKDDVYTHLYTLIAKPDNTYEVLIDNEKVES 580
S++ P Y S + I C D ++TL KPD T + EK +S
Sbjct: 56 FRSLLVSPELYKARSVSGHTESCLYLSIACYPDY--RMFTLCRKPDQTLTTSEEEEKKKS 113
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.9
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 277 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 387
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 2.5
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -3
Query: 365 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 237
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 29.5 bits (63), Expect = 3.3
Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = -1
Query: 184 PLGRLKNFPAVNLNFPNSFPGC-SLYTQLLSHES-SGNFSSKNTSQFIEDNASKLTT 20
PL +K+ P +++ PNS P C + + + L + +G K + +I NA +L T
Sbjct: 329 PLRSIKDQPNISVRKPNSVPECLTFHLETLEWQGYAGRPEDKEIAVYILGNALRLNT 385
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 5.7
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 371 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 270
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421:
Protein of unknown function (DUF751)
Length = 185
Score = 28.3 bits (60), Expect = 7.6
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Frame = +2
Query: 209 KMRGSMLSP---VSSNRSVMRVNPWSSSSLSNMNKTLTVEADTSRSLTANWSRRTCTER 376
KMR S L P +S+RS+ + SSS+ N L R LT SR + ER
Sbjct: 8 KMRLSCLQPHLHFTSSRSIAAALAFPSSSIRNRTSCLVRCGSARRRLTTKSSRNSKLER 66
>At2g36350.1 68415.m04461 protein kinase, putative similar to
protein kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 949
Score = 28.3 bits (60), Expect = 7.6
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +2
Query: 257 MRVNPWSSS----SLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRL 391
+R NP SS SLS+ N + TS S +N SR +C + HM +
Sbjct: 489 IRANPTSSEKFDFSLSSKNSLGDYSSSTSMSEESNLSRFSCGNKPHMSM 537
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,077,481
Number of Sequences: 28952
Number of extensions: 423009
Number of successful extensions: 1157
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1091
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1152
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2188225800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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