Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP02_FL5_A05
(923 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 191 1e-49
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 191 1e-49
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 191 1e-49
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 99 4e-22
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 98 1e-21
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 51 3e-07
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 45 2e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 37 0.003
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 36 0.006
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.008
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.008
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 29 0.70
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 4.9
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 6.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 8.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 191 bits (466), Expect = 1e-49
Identities = 88/98 (89%), Positives = 92/98 (93%)
Frame = +2
Query: 131 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 310
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 311 DKLKAERERGITIDIALWKFETXKYYVTIIDAPGTQRF 424
DKLKAERERGITIDIALWKFET KY VT+IDAPG + F
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDF 98
Score = 68.1 bits (159), Expect = 2e-12
Identities = 29/34 (85%), Positives = 32/34 (94%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTGDFEAGIS 515
HRDFIKNMITGTSQADCA+LI+ GTG+FEAGIS
Sbjct: 95 HRDFIKNMITGTSQADCAILIIGGGTGEFEAGIS 128
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 191 bits (466), Expect = 1e-49
Identities = 88/98 (89%), Positives = 92/98 (93%)
Frame = +2
Query: 131 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 310
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 311 DKLKAERERGITIDIALWKFETXKYYVTIIDAPGTQRF 424
DKLKAERERGITIDIALWKFET KY VT+IDAPG + F
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDF 98
Score = 68.5 bits (160), Expect = 1e-12
Identities = 30/34 (88%), Positives = 32/34 (94%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTGDFEAGIS 515
HRDFIKNMITGTSQADCAVLI+ GTG+FEAGIS
Sbjct: 95 HRDFIKNMITGTSQADCAVLIIGGGTGEFEAGIS 128
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 191 bits (466), Expect = 1e-49
Identities = 88/98 (89%), Positives = 92/98 (93%)
Frame = +2
Query: 131 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 310
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 311 DKLKAERERGITIDIALWKFETXKYYVTIIDAPGTQRF 424
DKLKAERERGITIDIALWKFET KY VT+IDAPG + F
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDF 98
Score = 68.1 bits (159), Expect = 2e-12
Identities = 29/34 (85%), Positives = 32/34 (94%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTGDFEAGIS 515
HRDFIKNMITGTSQADCA+LI+ GTG+FEAGIS
Sbjct: 95 HRDFIKNMITGTSQADCAILIIGGGTGEFEAGIS 128
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 99 bits (238), Expect = 4e-22
Identities = 44/90 (48%), Positives = 63/90 (70%)
Frame = +2
Query: 143 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 322
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 323 AERERGITIDIALWKFETXKYYVTIIDAPG 412
ERE+G T+++ FET +++DAPG
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPG 325
Score = 43.6 bits (98), Expect = 4e-05
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTGDFEAG 509
H+ ++ NMI G SQAD VL+++A G+FEAG
Sbjct: 326 HKGYVTNMINGASQADIGVLVISARRGEFEAG 357
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 98.3 bits (234), Expect = 1e-21
Identities = 45/94 (47%), Positives = 63/94 (67%)
Frame = +2
Query: 143 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 322
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 323 AERERGITIDIALWKFETXKYYVTIIDAPGTQRF 424
ER RG+T+D+A FE+ K I DAPG + F
Sbjct: 235 EERARGVTMDVASTTFESDKKIYEIGDAPGHRDF 268
Score = 40.3 bits (90), Expect = 4e-04
Identities = 18/32 (56%), Positives = 21/32 (65%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTGDFEAG 509
HRDFI MI G S AD AVL+V + +FE G
Sbjct: 265 HRDFISGMIAGASSADFAVLVVDSSQNNFERG 296
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 50.8 bits (116), Expect = 3e-07
Identities = 33/92 (35%), Positives = 46/92 (50%)
Frame = +2
Query: 137 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 316
++K H+NI IGHVD GK+T T I KC ++G+ SF +DK
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC--------------LSDLGQASFMDYSQIDK 93
Query: 317 LKAERERGITIDIALWKFETXKYYVTIIDAPG 412
E+ RGITI A ++ET + +D PG
Sbjct: 94 APEEKARGITISSAHVEYETANRHYAHVDCPG 125
Score = 35.1 bits (77), Expect = 0.014
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +3
Query: 414 HRDFIKNMITGTSQADCAVLIVAAGTG 494
H D+IKNMITG + D A+++V+A G
Sbjct: 126 HADYIKNMITGAATMDGAIIVVSATDG 152
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 44.8 bits (101), Expect = 2e-05
Identities = 30/90 (33%), Positives = 43/90 (47%)
Frame = +2
Query: 155 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 334
N+ +I H+D+GK+T T ++Y G F + G V+D L AER+
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG---------FTSHFGNVDTGDT----VMDYLPAERQ 76
Query: 335 RGITIDIALWKFETXKYYVTIIDAPGTQRF 424
RGITI+ A F + +ID PG F
Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPGHADF 106
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 37.1 bits (82), Expect = 0.003
Identities = 30/100 (30%), Positives = 44/100 (44%), Gaps = 4/100 (4%)
Frame = +2
Query: 155 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 334
N VI H+D GKST + ++ G I++ + F LDKL+ ER
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNF-----------RNQF-----LDKLEVERR 103
Query: 335 RGITIDI----ALWKFETXKYYVTIIDAPGTQRFHQEHDH 442
RGIT+ ++ + Y + +ID PG F E H
Sbjct: 104 RGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMH 143
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 36.3 bits (80), Expect = 0.006
Identities = 24/68 (35%), Positives = 36/68 (52%)
Frame = +2
Query: 155 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 334
NI + H+DSGK+T T ++Y G I K+ E+ +G +D ++ ERE
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELERE 110
Query: 335 RGITIDIA 358
+GITI A
Sbjct: 111 KGITIQSA 118
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.008
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +2
Query: 131 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 310
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 311 DKLKAERERGITI 349
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.008
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +2
Query: 131 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 310
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 311 DKLKAERERGITI 349
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 29.5 bits (63), Expect = 0.70
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -3
Query: 348 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIPPH 220
I IP+ A S+ N++ + +D LP + S V+L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -3
Query: 372 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMV 241
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.2 bits (55), Expect = 6.5
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +1
Query: 844 PPXGXXXXPXXXXXPXPXXPPPPXPP 921
PP P P PPPP PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/43 (23%), Positives = 22/43 (51%)
Frame = +2
Query: 353 IALWKFETXKYYVTIIDAPGTQRFHQEHDHRNLSG*LRCAHRS 481
+ +W F T + + + ++ ++ F + DHR + + C H S
Sbjct: 451 VRIWNFNTGEQHCVLHNSRNSRVFGLQFDHRRI---IACTHSS 490
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,698,042
Number of Sequences: 5004
Number of extensions: 46963
Number of successful extensions: 167
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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