Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_P16
(821 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 221 1e-58
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 184 1e-47
SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 32 0.11
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.8
SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosa... 26 5.6
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 7.4
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 7.4
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 9.8
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 25 9.8
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 9.8
SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomy... 25 9.8
SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 25 9.8
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 9.8
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 221 bits (539), Expect = 1e-58
Identities = 97/159 (61%), Positives = 120/159 (75%)
Frame = -3
Query: 630 ANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTM 451
A RI G+D+NP+K A FG +F+NP D PIQ VL+ TDGGL++TF+C GNV M
Sbjct: 221 AGRIFGIDVNPEKKNWAMSFGATDFINPNDLQSPIQDVLIHETDGGLDWTFDCTGNVHVM 280
Query: 450 RAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGGYKSRESVPKLVDEY 271
R+ALEACHKGWG S++IGVAAAG+EISTRPFQLVTGR W+G AFGG K R +P LV EY
Sbjct: 281 RSALEACHKGWGQSIVIGVAAAGQEISTRPFQLVTGRVWRGCAFGGVKGRSQLPDLVKEY 340
Query: 270 LEKKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVVDM 154
L+ KL +D+++TH PLKEINEAF MH G I+ V+ +
Sbjct: 341 LDHKLEIDKYITHRRPLKEINEAFTDMHNGNCIKTVLSI 379
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 184 bits (448), Expect = 1e-47
Identities = 85/162 (52%), Positives = 112/162 (69%)
Frame = -3
Query: 639 AAGANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNV 460
AAGA+RII +DIN DK AKKFG +F++ K + Q ++D+TDGG++Y F+C GNV
Sbjct: 215 AAGASRIIAIDINADKEVYAKKFGATDFIDSSKV-KDLVQYVIDVTDGGVDYAFDCTGNV 273
Query: 459 GTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGGYKSRESVPKLV 280
M+ L+ CHKGWG +IGVAAAG+ + RPF +VTGR G+AFGG K R +P V
Sbjct: 274 TVMQQELQFCHKGWGKLCVIGVAAAGKTLDFRPFLVVTGRQVLGSAFGGVKGRSELPNFV 333
Query: 279 DEYLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVVDM 154
DEY++ +DE++T+ PLK IN+AF MH GK IR VVDM
Sbjct: 334 DEYMQGHFKVDEYITNEEPLKNINKAFDHMHEGKCIRCVVDM 375
>SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 260
Score = 31.9 bits (69), Expect = 0.11
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -3
Query: 624 RIIGVDINPDKFEVAKKFGVNEFVNP 547
R++G+D +PD + A++ G+N +V P
Sbjct: 57 RVVGIDASPDMIKAARELGLNAYVIP 82
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.9 bits (59), Expect = 1.8
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +2
Query: 323 AVPFQVRPVTSWNGRVLISSPAAATPMIT 409
+VP+ PVTS N + S+P +TP+ T
Sbjct: 501 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 529
Score = 27.9 bits (59), Expect = 1.8
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +2
Query: 323 AVPFQVRPVTSWNGRVLISSPAAATPMIT 409
+VP+ PVTS N + S+P +TP+ T
Sbjct: 560 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 588
>SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase
Rdp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1215
Score = 26.2 bits (55), Expect = 5.6
Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = -3
Query: 630 ANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLV-DLTDGGLEYTFECIGN 463
ANR + V I + ++ + G + F N K Y + IQQ+L + G Y F GN
Sbjct: 485 ANRFMRVQITDEYYKQKIRGGSDGFRNEKLYSR-IQQLLTYGIKVGNQIYEFLAFGN 540
>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
Itr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = -3
Query: 417 GVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGG 313
G V+IG + G E++ + +T T G GG
Sbjct: 110 GALVVIGTSLGGHELTNGGKEFITSATSLGALLGG 144
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/31 (35%), Positives = 14/31 (45%), Gaps = 3/31 (9%)
Frame = -2
Query: 397 CSCCW---RRDQHSSIPTCYRSHLEGNSFWR 314
C CW + D + P C R + E N WR
Sbjct: 42 CRFCWHHIKEDLNGRCPACRRLYTEENVQWR 72
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.4 bits (53), Expect = 9.8
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = -3
Query: 528 IQQVLVDLTDGGLEYTFECIGNVGTMRAALEACH 427
I Q + DLT G + +C+ + A+L CH
Sbjct: 3280 IVQFITDLTVGEFQLCLKCLLSFSQHAASLRICH 3313
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1919
Score = 25.4 bits (53), Expect = 9.8
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -3
Query: 135 IYTIFVHLLNLFSSAFTTSIIKIHN 61
IYT+F HLLN++ S K N
Sbjct: 1720 IYTLFEHLLNIYKETINDSDKKEKN 1744
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 25.4 bits (53), Expect = 9.8
Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +2
Query: 368 VLISSPA-AATPMITDTPHPLWQASSAALMVPTFPIHSKVYSRPPSVK 508
VL++S A A T + H A +A +++P+F + SK+ + S+K
Sbjct: 1000 VLVNSEADTALRAKTTSQHLKQSAMAAKVVLPSFIVTSKISKQTKSIK 1047
>SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 557
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -3
Query: 456 TMRAALEACHKGWGVSVIIGVA-AAGEEIS 370
++ AA E H+GW + IG A A G+ IS
Sbjct: 227 SLNAAFEHVHQGWRIMFGIGAAPALGQLIS 256
>SPAC1952.17c ||SPAC890.01c|GTPase activating
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 619
Score = 25.4 bits (53), Expect = 9.8
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 219 KEINEAFHLMHAGKSIRAVVDM*FLRKNIY 130
K +N F L H K+ + DM ++RKN Y
Sbjct: 444 KLLNLTFELQHLRKTSKNDEDMSYVRKNSY 473
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 9.8
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +3
Query: 261 SSPSTHLQALVH-FLYFCNLQKLFPSRCD 344
SSPS+ L+ F Y CN+ KL+ + D
Sbjct: 914 SSPSSDLEVKQQAFTYVCNISKLYVYKSD 942
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,137,896
Number of Sequences: 5004
Number of extensions: 64296
Number of successful extensions: 184
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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