Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_O13
(783 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 58 5e-09
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 58 9e-09
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 44 1e-04
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 37 0.013
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 36 0.023
At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger) fa... 30 1.5
At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family prot... 29 3.5
At1g74010.1 68414.m08571 strictosidine synthase family protein s... 29 4.6
At4g33750.1 68417.m04792 expressed protein 28 6.1
At3g57600.1 68416.m06417 AP2 domain-containing transcription fac... 28 6.1
At2g02230.1 68415.m00161 F-box family protein / SKP1 interacting... 28 6.1
At4g31820.1 68417.m04522 phototropic-responsive NPH3 family prot... 28 8.0
At4g29380.1 68417.m04197 protein kinase family protein / WD-40 r... 28 8.0
At3g04770.1 68416.m00513 40S ribosomal protein SA (RPSaB) identi... 28 8.0
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 58.4 bits (135), Expect = 5e-09
Identities = 27/83 (32%), Positives = 42/83 (50%)
Frame = -1
Query: 396 KVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQELLGAKNYPS 217
K+ P+ + L Y+LD IY+W+G+ +E+ A A+E L ++N P
Sbjct: 261 KMEPIDGDLSKSMLENTKCYLLDC-GAEIYIWVGRVTQVDERKAASQSAEEFLASENRPK 319
Query: 216 WVQVTRVLQNTEPAAFKQYFFTW 148
VTRV+Q E +FK F +W
Sbjct: 320 ATHVTRVIQGYESHSFKSNFDSW 342
Score = 41.1 bits (92), Expect = 8e-04
Identities = 19/60 (31%), Positives = 33/60 (55%)
Frame = -2
Query: 782 MFQXKGKRXVXVRQVDPQIASMXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQVR 603
++ KGKR + ++QV +S+ DVF+LD + IY F G + E+ KA+ ++
Sbjct: 139 LYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNGANSNIQERAKALEVVQYLK 198
Score = 28.7 bits (61), Expect = 4.6
Identities = 21/79 (26%), Positives = 36/79 (45%)
Frame = -1
Query: 477 GGDDQEFERNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWI 298
GG F+ E V + + GK + PF + +L+ + +ILDT IY +
Sbjct: 121 GGVASGFKTVE-EEVFETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDT-EEKIYQFN 178
Query: 297 GKQATANEKSQAMTKAQEL 241
G + E+++A+ Q L
Sbjct: 179 GANSNIQERAKALEVVQYL 197
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 57.6 bits (133), Expect = 9e-09
Identities = 44/160 (27%), Positives = 74/160 (46%), Gaps = 2/160 (1%)
Frame = -1
Query: 621 VRQSSTDQDHHGRGRVDIVD--KYSSDVDVQKFFTALGSGVKDLVPDESTGGDDQEFERN 448
V Q D+ H G V IVD K ++ D +F+ G G + +D+
Sbjct: 195 VIQYLKDKFHEGTSDVAIVDDGKLDTESDSGEFWVLFG-GFAPIA--RKVASEDEIIP-- 249
Query: 447 EASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKS 268
E + L ++D + LSK + N Y+LD S I++W+G+ E+
Sbjct: 250 ETTPPKLYSIADGQVESIDGDLSKSMLENN----KCYLLDCGS-EIFIWVGRVTQVEERK 304
Query: 267 QAMTKAQELLGAKNYPSWVQVTRVLQNTEPAAFKQYFFTW 148
A+ A++ + ++N P ++TRV+Q EP +FK F +W
Sbjct: 305 TAIQAAEDFVASENRPKATRITRVIQGYEPHSFKSNFDSW 344
Score = 39.9 bits (89), Expect = 0.002
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = -2
Query: 782 MFQXKGKRXVXVRQVDPQIASMXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAI 624
++ KGKR V ++QV +S+ DVF+LD IY F G + E+ KA+
Sbjct: 141 LYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNGANSNIQERAKAL 193
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = -1
Query: 405 GKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANEKSQAMTKAQEL 241
GK V PF + +L+ + +ILDT IY + G + E+++A+ Q L
Sbjct: 146 GKRAVHLKQVPFARSSLNHDDVFILDT-KEKIYQFNGANSNIQERAKALVVIQYL 199
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 44.0 bits (99), Expect = 1e-04
Identities = 30/110 (27%), Positives = 54/110 (49%), Gaps = 4/110 (3%)
Frame = -1
Query: 465 QEFERNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQA 286
Q+ +R+ S+ L + +K T + F Q++L ++ +ILD + ++VW+G+Q
Sbjct: 614 QKIKRDGESDPHLFSCTYTNESLKATEIFN-FTQDDLMTEDIFILDCHT-EVFVWVGQQV 671
Query: 285 TANEKSQAMTKAQELLG----AKNYPSWVQVTRVLQNTEPAAFKQYFFTW 148
+K QA+ + L +N S + V + EP F + FFTW
Sbjct: 672 DPKKKPQALDIGENFLKHDFLLENLASETPIYIVTEGNEPPFFTR-FFTW 720
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/60 (30%), Positives = 34/60 (56%)
Frame = -2
Query: 782 MFQXKGKRXVXVRQVDPQIASMXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQVR 603
++ KGK V V++V +++ DVF+LD ++ I+ F G K+ E+ KA+ ++
Sbjct: 141 LYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIK 200
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 37.1 bits (82), Expect = 0.013
Identities = 17/60 (28%), Positives = 32/60 (53%)
Frame = -2
Query: 782 MFQXKGKRXVXVRQVDPQIASMXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQVR 603
+ + KG V V++V +S+ DVF+LD + +++F G + EK KA+ ++
Sbjct: 140 LLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSSTQEKAKAMEVVEYIK 199
Score = 37.1 bits (82), Expect = 0.013
Identities = 40/166 (24%), Positives = 72/166 (43%), Gaps = 7/166 (4%)
Frame = -1
Query: 624 FVRQSSTDQDHHGRGRVDIVDKYSSDVDVQKFFTALGSGVK---DLVPDESTGGDDQEFE 454
++ + S+D DH R+ Y D Q + G+ +L+ +S ++E
Sbjct: 558 WIGKLSSDSDHEVLDRM----LYFLDTSCQPIYIREGNETDTFWNLLGGKSEYPKEKEM- 612
Query: 453 RNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTISGNIYVWIGKQATANE 274
R + L S ++ +KV + F Q++L+ ++ ++LD S +YVWIG +
Sbjct: 613 RKQIEEPHLFTCSCSSDVLKVKEIYN-FVQDDLTTEDVFLLDCQS-EVYVWIGSNSNIKS 670
Query: 273 KSQAMTKAQELLG----AKNYPSWVQVTRVLQNTEPAAFKQYFFTW 148
K +A+T + L + V V + EP F + FF W
Sbjct: 671 KEEALTLGLKFLEMDILEEGLTMRTPVYVVTEGHEPPFFTR-FFEW 715
Score = 35.1 bits (77), Expect = 0.053
Identities = 40/160 (25%), Positives = 68/160 (42%), Gaps = 2/160 (1%)
Frame = -1
Query: 540 VQKFFTALGSGVKDLVPDESTGGDDQEFERNEASNVILSEVS-DATGKIKVTPLSKPFKQ 364
V F G V++L PDE +D N N+ + V D + + +K F
Sbjct: 361 VAALFKQKGYDVEEL-PDE----EDDPLYTNCRDNLKVWRVDGDDVSLLSIPDQTKLFTG 415
Query: 363 ENLSPQNAYILDTISGNI-YVWIGKQATANEKSQAMTKAQELLGAKNYPSWVQVTRVLQN 187
+ Q Y + ++ YVWIG ++ +++ A+T A ++G S + + Q
Sbjct: 416 DCYLVQYKYTYKERTEHLLYVWIGCESIQQDRADAITNASAIVGTTKGES--VLCHIYQG 473
Query: 186 TEPAAFKQYFFTWRDFGMSHSRVI*KLKAQNKEKVP*DYH 67
EP+ F F + F SR K+ KEK+ +Y+
Sbjct: 474 NEPSRFFPMFQSLVVFKGGLSRRY-KVLLAEKEKIGEEYN 512
Score = 27.9 bits (59), Expect = 8.0
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = -2
Query: 707 DVFVLDXDNDIYVFVGEKAKNVEKLKAIS 621
DVF+LD +++YV++G + K +A++
Sbjct: 648 DVFLLDCQSEVYVWIGSNSNIKSKEEALT 676
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 36.3 bits (80), Expect = 0.023
Identities = 16/60 (26%), Positives = 32/60 (53%)
Frame = -2
Query: 782 MFQXKGKRXVXVRQVDPQIASMXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQVR 603
+F +GK V V++V +S+ D+++LD + I+ F G + E+ KA+ ++
Sbjct: 141 LFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVVQYIK 200
Score = 29.5 bits (63), Expect = 2.6
Identities = 24/84 (28%), Positives = 41/84 (48%)
Frame = -1
Query: 498 LVPDESTGGDDQEFERNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQNAYILDTIS 319
++P E GG F+ A I + + GK V PF + +L+ + YILDT S
Sbjct: 118 IIPQE--GGVASGFKHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKS 174
Query: 318 GNIYVWIGKQATANEKSQAMTKAQ 247
I+ + G ++ E+++A+ Q
Sbjct: 175 -KIFQFNGSNSSIQERAKALEVVQ 197
Score = 28.7 bits (61), Expect = 4.6
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = -2
Query: 707 DVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQ 609
D+F++D ++I+V+VG++ KL A++ +
Sbjct: 651 DIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEK 683
>At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 645
Score = 30.3 bits (65), Expect = 1.5
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 3/57 (5%)
Frame = -1
Query: 600 QDHHGRGRVDIVDKYSSDVDVQKFFTALGSGVKDLVPDESTGGD---DQEFERNEAS 439
Q +G V + Y D D ++F GSG +DL ++ T + DQ+FE++ A+
Sbjct: 306 QSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSFDQKFEKSNAA 362
>At3g26430.1 68416.m03294 GDSL-motif lipase/hydrolase family protein
similar to early nodulin ENOD8 [Medicago sativa]
GI:304037; contains InterPro Entry IPR001087 Lipolytic
enzyme, G-D-S-L family
Length = 380
Score = 29.1 bits (62), Expect = 3.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 250 SFRHGLRFLVCGSLLPDPNVNVARYGVQNI 339
+F HG F GS + PN +A+ GV I
Sbjct: 97 NFSHGANFATAGSTVRPPNATIAQSGVSPI 126
>At1g74010.1 68414.m08571 strictosidine synthase family protein
similar to strictosidine synthase [Rauvolfia
serpentina][SP|P15324]; contains strictosidine synthase
domain PF03088
Length = 325
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 459 FERNEASNVILSEVSDATGKIKVTPLSKPF 370
F +NE N +LSEV++ G + + L+ PF
Sbjct: 290 FLKNEFGNTLLSEVNEFNGHLYIGTLTGPF 319
>At4g33750.1 68417.m04792 expressed protein
Length = 148
Score = 28.3 bits (60), Expect = 6.1
Identities = 14/57 (24%), Positives = 26/57 (45%)
Frame = -1
Query: 516 GSGVKDLVPDESTGGDDQEFERNEASNVILSEVSDATGKIKVTPLSKPFKQENLSPQ 346
GS ++ + + GDD E E+SN+ +E+++ T +S+ PQ
Sbjct: 67 GSNTTEVNENSRSEGDDDESGEKESSNIFSTELTEEADNTSGTSISQEEMDALTDPQ 123
>At3g57600.1 68416.m06417 AP2 domain-containing transcription
factor, putative various proteins containing an AP2
transcription factor domain, Arabidopsis thaliana
Length = 277
Score = 28.3 bits (60), Expect = 6.1
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = -1
Query: 306 VWIGKQATANEKSQAMTKAQELLGAKNYPSWVQVTRVLQNTEPAAFKQYFFTW 148
+W+G ATA E + A +A L + +++ + + +NT P+ F W
Sbjct: 52 LWLGSFATAEEAAMAYDEA--ALKLYGHDAYLNLPHLQRNTRPSLSNSQRFKW 102
>At2g02230.1 68415.m00161 F-box family protein / SKP1 interacting
partner 3-related contains similarity to SKP1
interacting partner 3 GI:10716951 from [Arabidopsis
thaliana]
Length = 317
Score = 28.3 bits (60), Expect = 6.1
Identities = 27/95 (28%), Positives = 43/95 (45%), Gaps = 5/95 (5%)
Frame = -2
Query: 719 MXKGDVFVLDXDNDIYVFVGEKAKNVEKLKAISFANQVRIRIITDAEESILWTSTRAMW- 543
+ K ++F+ DN + V G+K+ VEK A+ + +++ E +I+W + A W
Sbjct: 91 LSKKEIFLSLADNSVLVENGKKSFWVEK------ASGKKCYMLSAMELTIIWGDSPAYWK 144
Query: 542 --TFRNSSRLLVQELR--IWCRMRAPAETTRSSKG 450
T S V ELR W +R SKG
Sbjct: 145 WITVPESKFEKVAELRNVCWFEVRGKISCGMLSKG 179
>At4g31820.1 68417.m04522 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 571
Score = 27.9 bits (59), Expect = 8.0
Identities = 13/41 (31%), Positives = 26/41 (63%)
Frame = -2
Query: 698 VLDXDNDIYVFVGEKAKNVEKLKAISFANQVRIRIITDAEE 576
V D D+D+ + VGE ++ K +S +N+++ R++ +A E
Sbjct: 24 VSDLDSDVTIHVGEVTFHLHKFPLLSKSNRMQ-RLVFEASE 63
>At4g29380.1 68417.m04197 protein kinase family protein / WD-40 repeat
family protein contains Pfam PF00400: WD domain, G-beta
repeat; contains Pfam PF00069: Protein kinase domain;
contains PF02985: HEAT repeat; similar to adaptor protein
(GI:1817584) [Homo sapiens]; similar to VPS15 protein
(GI:6103009) [Pichia pastoris]
Length = 1494
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -1
Query: 597 DHHGRGRVDIVDKYSSDVDVQK 532
DH RG ++V+KYS VD++K
Sbjct: 1159 DHISRGLGNVVEKYSGIVDIKK 1180
>At3g04770.1 68416.m00513 40S ribosomal protein SA (RPSaB) identical
to p40 protein homolog GB:AAB67866 [Arabidopsis
thaliana]; similar to 40S ribosomal protein SA (P40)
GB:O65751 [Cicer arietinum]
Length = 332
Score = 27.9 bits (59), Expect = 8.0
Identities = 23/74 (31%), Positives = 42/74 (56%), Gaps = 10/74 (13%)
Frame = +1
Query: 457 ELLVVSAGALIRHQILNS*TKSREEF-LNVHIA-------RVLVHN--IDSSASVMILIR 606
E LVV++G L+++ +L+ KS +F L++ +A R+L+ + S +S++ L R
Sbjct: 256 EWLVVTSGPLLKYLMLHGLVKSNSQFLLHLQLALPLLLDGRLLLFQLLVGSKSSLISLAR 315
Query: 607 T*LANEIALSFSTF 648
T L ++ FS F
Sbjct: 316 TRLDVDLIFLFSRF 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,401,545
Number of Sequences: 28952
Number of extensions: 286885
Number of successful extensions: 836
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 836
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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