Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_N02
(808 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 262 4e-71
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 258 9e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 103 3e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 57 3e-09
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.19
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 7.2
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 7.2
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 7.2
SPAC30D11.11 |||Haemolysin-III family protein|Schizosaccharomyce... 25 9.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.6
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 9.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 262 bits (642), Expect = 4e-71
Identities = 115/142 (80%), Positives = 129/142 (90%)
Frame = -3
Query: 554 PPXQMVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 375
P QMVKCDPR G+YMA C+LYRGDV+PRDV AA+ +IK++RTIQFVDWCPTGFK+GI Y
Sbjct: 302 PYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICY 361
Query: 374 QPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGE 195
+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGE
Sbjct: 362 EPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGE 421
Query: 194 FSEAREDLAALEKDYEEVGMDS 129
FSEAREDLAALE+DYEEVG DS
Sbjct: 422 FSEAREDLAALERDYEEVGQDS 443
Score = 65.7 bits (153), Expect = 7e-12
Identities = 27/36 (75%), Positives = 31/36 (86%)
Frame = -2
Query: 660 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 553
PRIHFPLVTY+P++SA KA+HE SV EITN CFEP
Sbjct: 267 PRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 34.7 bits (76), Expect = 0.016
Identities = 20/41 (48%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 793 DXEXPTYPNLXXXXDXFXLD-HCFLRFDGALNXDLXEFQTN 674
D E PTY NL LRF G+LN DL EFQTN
Sbjct: 222 DIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTN 262
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 258 bits (631), Expect = 9e-70
Identities = 114/142 (80%), Positives = 127/142 (89%)
Frame = -3
Query: 554 PPXQMVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 375
P QMVKCDPR G+YMA C+LYRGDV+PRDV AA+ TIK KRTIQFVDWCPTGFK+GI
Sbjct: 298 PYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICD 357
Query: 374 QPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGE 195
+PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGE
Sbjct: 358 RPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGE 417
Query: 194 FSEAREDLAALEKDYEEVGMDS 129
FSEAREDLAALE+DYEEVG DS
Sbjct: 418 FSEAREDLAALERDYEEVGQDS 439
Score = 66.9 bits (156), Expect = 3e-12
Identities = 28/36 (77%), Positives = 31/36 (86%)
Frame = -2
Query: 660 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 553
PRIHFPLVTYAP++SA KA+HE SV EITN CFEP
Sbjct: 263 PRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298
Score = 34.7 bits (76), Expect = 0.016
Identities = 19/41 (46%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = -1
Query: 793 DXEXPTYPNLXXXXDXFXLD-HCFLRFDGALNXDLXEFQTN 674
D E P+Y NL LRF+G+LN DL EFQTN
Sbjct: 218 DIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTN 258
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 103 bits (247), Expect = 3e-23
Identities = 52/140 (37%), Positives = 81/140 (57%), Gaps = 3/140 (2%)
Frame = -3
Query: 542 MVKCDPRHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 363
MV DPRHG+Y+ L+RG V ++V+ I +++TK + FV+W P + PP
Sbjct: 300 MVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK 359
Query: 362 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 183
DL + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 -----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEA 411
Query: 182 R---EDLAALEKDYEEVGMD 132
DL + + Y+E G+D
Sbjct: 412 ESNMNDLVSEYQQYQEAGID 431
Score = 30.3 bits (65), Expect = 0.34
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -2
Query: 660 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 556
PR+HF +V +AP+ + + + +SV E+T F+
Sbjct: 261 PRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFD 295
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 57.2 bits (132), Expect = 3e-09
Identities = 36/140 (25%), Positives = 71/140 (50%), Gaps = 4/140 (2%)
Frame = -3
Query: 554 PPXQMVKCDP-RHGKYMACCMLYRGDVVPRDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 378
P QMV +P + +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 377 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE- 201
+ P + ++ + ML+N T+IA + R ++D + + AF+ Y E + E
Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFED 416
Query: 200 --GEFSEAREDLAALEKDYE 147
EF +R+ +A L +YE
Sbjct: 417 DLNEFDSSRDVVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 31.1 bits (67), Expect = 0.19
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -1
Query: 487 VVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 353
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 25.4 bits (53), Expect = 9.6
Identities = 14/45 (31%), Positives = 18/45 (40%)
Frame = -1
Query: 487 VVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 353
VV P + RP++P P S PV V + PP P
Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 283 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 110
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 109 EPKSTK 92
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.5
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -1
Query: 451 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 365
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 4/36 (11%)
Frame = -3
Query: 614 PRRPTMNSFPSPR----SQTHASSPPXQMVKCDPRH 519
P RP + + P P+ S HA PP Q + P H
Sbjct: 1356 PVRPAVPTSPKPQIPDSSNVHAPPPPVQPMNAMPSH 1391
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 7.2
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +2
Query: 470 WVRRHHGTAYSKPCTCHDGG 529
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 25.8 bits (54), Expect = 7.2
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2
Query: 588 SVAEITNACFEPAXPDGEMRPPSWQV 511
SV ++T ACFEP+ ++ P W +
Sbjct: 800 SVTKVTCACFEPSLDYVVVKIPRWDL 825
>SPAC30D11.11 |||Haemolysin-III family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 25.4 bits (53), Expect = 9.6
Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +3
Query: 465 IPG-YDVTTVQHTASHVLAMTGVAFHH 542
+PG +D+ H H+ + GVAFH+
Sbjct: 398 LPGKFDIIGNSHQIWHIAIIVGVAFHY 424
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.6
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -1
Query: 454 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 359
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 530
Score = 25.4 bits (53), Expect = 9.6
Identities = 19/60 (31%), Positives = 26/60 (43%)
Frame = -1
Query: 532 ATPVMASTWLAVCCTVVTSYPGM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 353
+TP ++S A VV+S T + S S ++SS P S SVS W P
Sbjct: 245 STPAVSSA-AASSSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSVSGKRGLAWIP 303
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,970,113
Number of Sequences: 5004
Number of extensions: 61076
Number of successful extensions: 225
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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