Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_M24
(863 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 225 5e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 222 4e-59
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 9e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 8e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.013
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.5
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 6.0
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 6.0
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 225 bits (551), Expect = 5e-60
Identities = 100/128 (78%), Positives = 115/128 (89%), Gaps = 1/128 (0%)
Frame = -3
Query: 516 WLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV 340
++A C RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKV 375
Query: 339 QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD 160
RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+D
Sbjct: 376 NRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 435
Query: 159 YEEVGMDS 136
YEEVG DS
Sbjct: 436 YEEVGQDS 443
Score = 67.7 bits (158), Expect = 2e-12
Identities = 28/37 (75%), Positives = 30/37 (81%)
Frame = -2
Query: 607 HEQXSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 497
HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 287 HESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323
Score = 53.6 bits (123), Expect = 3e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = -1
Query: 761 QIXSXITXXLXFXGRF*MWTSPXXQXNLVPYPRIHFPLVTYAPVXXAEKAY 609
Q+ S IT L F G + + Q NLVPYPRIHFPLVTY+P+ A KA+
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEF-QTNLVPYPRIHFPLVTYSPIVSAAKAF 286
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 222 bits (543), Expect = 4e-59
Identities = 100/131 (76%), Positives = 114/131 (87%), Gaps = 1/131 (0%)
Frame = -3
Query: 525 ASTWLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 349
A ++A C RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 348 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 169
AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL
Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428
Query: 168 EKDYEEVGMDS 136
E+DYEEVG DS
Sbjct: 429 ERDYEEVGQDS 439
Score = 67.7 bits (158), Expect = 2e-12
Identities = 28/37 (75%), Positives = 30/37 (81%)
Frame = -2
Query: 607 HEQXSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 497
HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 283 HESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319
Score = 54.8 bits (126), Expect = 1e-08
Identities = 28/51 (54%), Positives = 33/51 (64%)
Frame = -1
Query: 761 QIXSXITXXLXFXGRF*MWTSPXXQXNLVPYPRIHFPLVTYAPVXXAEKAY 609
Q+ S IT L F G + + Q NLVPYPRIHFPLVTYAP+ A KA+
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEF-QTNLVPYPRIHFPLVTYAPIVSAAKAF 282
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 82.2 bits (194), Expect = 9e-17
Identities = 44/122 (36%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = -3
Query: 495 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 316
RG V K+V+ I +++TK + FV+W P + PP DL + + +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIG 369
Query: 315 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVG 145
N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y+E G
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 144 MD 139
+D
Sbjct: 430 ID 431
Score = 37.9 bits (84), Expect = 0.002
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -2
Query: 595 SVAEITNACFEPANQMVKCDPRHGKYMACCMLY 497
SV E+T F+ N MV DPRHG+Y+ L+
Sbjct: 285 SVPELTQQMFDANNMMVAADPRHGRYLTVAALF 317
Score = 30.3 bits (65), Expect = 0.37
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = -1
Query: 683 NLVPYPRIHFPLVTYAPV 630
N+VP+PR+HF +V +AP+
Sbjct: 256 NMVPFPRLHFFMVGFAPL 273
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.4 bits (120), Expect = 8e-08
Identities = 34/140 (24%), Positives = 69/140 (49%), Gaps = 4/140 (2%)
Frame = -3
Query: 561 PPTRW*NATPVMASTWLAVC-CTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 385
P + + P S ++++ +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 384 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE- 208
+ P + ++ + ML+N T+IA + R ++D + + AF+ Y E + E
Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFED 416
Query: 207 --GEFSEAREDLAALEKDYE 154
EF +R+ +A L +YE
Sbjct: 417 DLNEFDSSRDVVADLINEYE 436
Score = 25.8 bits (54), Expect = 7.9
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = -1
Query: 683 NLVPYPRIHFPLVTYAP 633
+L+P PR HF L +Y P
Sbjct: 259 SLIPSPRCHFLLTSYTP 275
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 35.1 bits (77), Expect = 0.013
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -1
Query: 503 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 360
A PVV P + RP++P P LS V PV+ V + PP P
Sbjct: 549 AAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 29.5 bits (63), Expect = 0.64
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -1
Query: 503 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 360
A PVV P + RP++P P S PV V + PP P
Sbjct: 639 AAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/49 (36%), Positives = 21/49 (42%)
Frame = -1
Query: 500 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 354
VP V S P+ P +P PSV + V P SV P PEA
Sbjct: 643 VPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEA 691
Score = 26.6 bits (56), Expect = 4.5
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = -1
Query: 497 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 357
P V S P+ P +P PSV + V P + SV P PE
Sbjct: 539 PEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPE 585
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -1
Query: 500 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 357
VP S P+ P +P PSV + V P + SV P PE
Sbjct: 628 VPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 675
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 290 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 117
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 116 EPKSTK 99
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 6.0
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -1
Query: 458 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 372
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 6.0
Identities = 26/101 (25%), Positives = 40/101 (39%)
Frame = -1
Query: 497 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWPRFNVXXXXX 318
PVV + P T+P S P+ S + PVS + T + PP A R N
Sbjct: 988 PVVQNKPAA-TKPV--SMPAAKSKPAPMANPVSTAQQTQNRPP--APAMQARPNTTQAAA 1042
Query: 317 XXXXXXPKLGLALTTSSTSCTPSVLSCTGTSVRVWRRESSP 195
+ A T S++ PS ++ +S + S+P
Sbjct: 1043 PVTSTTTTIKQATTVSASKPAPSTVTSAASSPSNISKPSAP 1083
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,970,940
Number of Sequences: 5004
Number of extensions: 59465
Number of successful extensions: 223
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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