Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_M21
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 237 2e-63
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 117 2e-27
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 103 3e-23
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 101 1e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 1e-20
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 69 1e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 68 2e-12
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 68 2e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.8
SPBC14C8.05c |meu17||glucan-alpha-1,4-glucosidase|Schizosaccharo... 27 4.0
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 7.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 237 bits (579), Expect = 2e-63
Identities = 112/147 (76%), Positives = 120/147 (81%)
Frame = -2
Query: 771 TXXSSXSXENFTNFPTVRSSPSETKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVD 592
T S S E P + +RFR PEALFQPS LG+E GIHE TYNSIMKCDVD
Sbjct: 229 TAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVD 288
Query: 591 IRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS 412
IRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILAS
Sbjct: 289 IRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILAS 348
Query: 411 LSTFQQMWISKQEYDESGPSIVHRKCF 331
LSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 349 LSTFQQMWISKQEYDESGPGIVYRKCF 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 117 bits (282), Expect = 2e-27
Identities = 53/124 (42%), Positives = 83/124 (66%)
Frame = -2
Query: 702 TKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD 523
T+ F PE LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +
Sbjct: 257 TECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGE 316
Query: 522 RMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVH 343
R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++
Sbjct: 317 RFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIF 375
Query: 342 RKCF 331
R+ F
Sbjct: 376 RRRF 379
Score = 26.2 bits (55), Expect = 5.4
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -1
Query: 739 YEFPDGQVITIGNEKIPLPRGSFPTLVLG 653
Y+ PD Q IT+G E P F ++G
Sbjct: 245 YQLPDNQTITLGTECFSAPEVLFNPEMMG 273
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 103 bits (247), Expect = 3e-23
Identities = 59/151 (39%), Positives = 82/151 (54%), Gaps = 11/151 (7%)
Frame = -2
Query: 777 DXTXXSSXSXENFTNFPTVRSSPSETKRFRCPEALFQPSFLGMEAC---------GIHET 625
D SS + + F FP + +RFR E LF PSF + G+HE
Sbjct: 269 DEAKASSRNPKPF-EFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHEL 327
Query: 624 TYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPER 451
Y SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S + +
Sbjct: 328 VYQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASAT 387
Query: 450 KYSVWIGGSILASLSTFQQMWISKQEYDESG 358
+VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 388 SNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 101 bits (242), Expect = 1e-22
Identities = 57/141 (40%), Positives = 85/141 (60%), Gaps = 12/141 (8%)
Frame = -2
Query: 744 NFTNFPTVRSSPSETKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANT 565
N+T P R ++R+ CPE LFQP +G E G+ E +++I DVDIRK LY
Sbjct: 239 NYT-LPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAI 297
Query: 564 VLSGGTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSIL 418
VLSGG++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++L
Sbjct: 298 VLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVL 357
Query: 417 AS-LSTFQQMWISKQEYDESG 358
A ++ MW+SK E++E G
Sbjct: 358 ADIMAQNDHMWVSKAEWEEYG 378
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.1 bits (226), Expect = 1e-20
Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = -2
Query: 639 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 460
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 459 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 334
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 68.5 bits (160), Expect = 1e-12
Identities = 30/118 (25%), Positives = 62/118 (52%)
Frame = -2
Query: 699 KRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 520
++ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 519 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 346
+++E+T++ P I + W G S + F+ ++++EY E GP +
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 67.7 bits (158), Expect = 2e-12
Identities = 38/121 (31%), Positives = 59/121 (48%)
Frame = -2
Query: 699 KRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 520
+RF PE LF PS + + GI E S+ +I+ L N V GG +PG R
Sbjct: 279 ERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKR 338
Query: 519 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 340
+ E+ +LAP+ ++K+ P + W S + L + I++ EY E G +I+ R
Sbjct: 339 LSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTR 397
Query: 339 K 337
K
Sbjct: 398 K 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 67.7 bits (158), Expect = 2e-12
Identities = 38/135 (28%), Positives = 64/135 (47%), Gaps = 15/135 (11%)
Frame = -2
Query: 699 KRFRCPEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD 523
+RF PE F P + + E N + +D+RK LY N VLSGG+T++ +
Sbjct: 285 ERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGN 344
Query: 522 RMQKEITALAPST--------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWI 385
R+Q+++ + + + +I+ ++ +VW GGS+LA F
Sbjct: 345 RLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCH 404
Query: 384 SKQEYDESGPSIVHR 340
+K +Y+E G SI R
Sbjct: 405 TKADYEEYGASIARR 419
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -2
Query: 585 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 415
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 414 SLSTFQQMWISKQEYDESGPSIVHRK 337
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.8
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -2
Query: 438 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 337
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPBC14C8.05c
|meu17||glucan-alpha-1,4-glucosidase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 450
Score = 26.6 bits (56), Expect = 4.0
Identities = 16/57 (28%), Positives = 25/57 (43%)
Frame = +2
Query: 560 NTVLAYKSLRMSTSHFMMELYVVSWMPQASIPKNEGWKRASGQRNLFVSDGDDLTVG 730
+T+LA H + L + P N+GWK+A G+ V DG ++G
Sbjct: 279 STLLASLYSNEFDMHILPTLLKLQETMTRDYPVNQGWKQAMGRYPEDVYDGVSKSIG 335
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 513 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 403
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,786,724
Number of Sequences: 5004
Number of extensions: 56796
Number of successful extensions: 187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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