Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_M14
(799 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 231 1e-61
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 46 6e-06
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 31 0.25
SPAC644.09 |||alanine racemase|Schizosaccharomyces pombe|chr 1||... 28 1.3
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 27 3.1
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 27 3.1
SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr... 26 5.4
SPCC594.02c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.4
SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 7.2
SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa... 25 9.5
SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa... 25 9.5
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 231 bits (564), Expect = 1e-61
Identities = 102/156 (65%), Positives = 126/156 (80%)
Frame = -3
Query: 515 QTEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVVADRGLGLADLITVLDAFFKRLGFD 336
Q F P KYFSID+VFRNET+DATHLAEFHQVEGV+ DR + L DLI L+ FF ++
Sbjct: 342 QNGFHPAKYFSIDRVFRNETVDATHLAEFHQVEGVICDRNITLGDLIGFLEVFFGKMNVK 401
Query: 335 QLQFKPAYNPYTEPSMEIFAYHTGLAKWIEIGNSGVFRPEMLLPMGLPEDVNVIAWGLSL 156
L+FKPAYNPYTEPS+E+F+YH L KW+E+GNSG+FRPEML PMGLP+DV + +GLSL
Sbjct: 402 NLRFKPAYNPYTEPSLEVFSYHEKLGKWVEVGNSGMFRPEMLEPMGLPKDVRCLGFGLSL 461
Query: 155 ERPTMIKYGLNNIRDLVGPKVDLRMVYNNPICRLDK 48
ERPTMIKYG+ +IR L+GPKV+L ++ +P RLDK
Sbjct: 462 ERPTMIKYGVADIRQLIGPKVNLDLIEASPAVRLDK 497
Score = 41.1 bits (92), Expect = 2e-04
Identities = 21/38 (55%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
Frame = -2
Query: 642 HSKGGYGSQ-GYRYDWKIEEAQKNLLRTHTTAVSARML 532
H GG GYR + +EE +K +LRTHTTAVSA ML
Sbjct: 300 HENGGETKGIGYRAPFSLEETRKLVLRTHTTAVSANML 337
Score = 35.1 bits (77), Expect = 0.012
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -1
Query: 751 FQPQQXPARDXHDTFFXSSPAATTEFP 671
F PQQ ARD DTFF PA+T + P
Sbjct: 262 FVPQQHSARDAQDTFFLKVPASTDKLP 288
>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 429
Score = 46.0 bits (104), Expect = 6e-06
Identities = 30/81 (37%), Positives = 46/81 (56%), Gaps = 4/81 (4%)
Frame = -3
Query: 344 GFDQLQFKPAYN--PYTEPSMEIFAYHTGLAKWIEIGNSGVFRPEMLLPMGLPEDVNVIA 171
G +Q++++ Y+ P+T+PS ++ G KW+EI GV + +L GL N I
Sbjct: 239 GAEQVRYRWTYDSFPFTKPSFQLEIDWKG--KWLEILGCGVVQDRLLKGAGLN---NYIG 293
Query: 170 W--GLSLERPTMIKYGLNNIR 114
W G+ LER MI YG+ +IR
Sbjct: 294 WAFGIGLERLAMILYGIPDIR 314
Score = 27.9 bits (59), Expect = 1.8
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = -3
Query: 527 RLAQQTEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVV 408
RLA++ F + V+R + +D++H FHQ+EG +
Sbjct: 124 RLAKEG-FKKNGFLITADVYRRDEVDSSHYPIFHQMEGAL 162
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 30.7 bits (66), Expect = 0.25
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = -3
Query: 494 KYFSIDKVFRNETLDATHLAEFHQVEGVVA 405
+ F + K FRNE +DATH EF E A
Sbjct: 273 RVFELGKNFRNEGIDATHNPEFTSCEAYCA 302
>SPAC644.09 |||alanine racemase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 237
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +1
Query: 130 PYLIMVGRSRDRPHAITFTSSGRPMGSSISGLNTPELPISIHLA 261
P + +G S D AI + S +GSSI G E P +H++
Sbjct: 191 PLQLSMGMSSDYLLAIKYGSDSVRVGSSIFGSRPTEKPSDVHIS 234
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 27.1 bits (57), Expect = 3.1
Identities = 7/25 (28%), Positives = 16/25 (64%)
Frame = +3
Query: 600 NRICNPGSHIHLYCALLHPLXVXMG 674
+++ PG ++ + C L+HP+ + G
Sbjct: 393 DKMVMPGDNVEMICTLIHPIVIEKG 417
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -3
Query: 494 KYFSIDKVFRNETLDATHLAEFHQVE 417
+ + I + FRNE D TH EF +E
Sbjct: 318 RVYEIGRQFRNEGADLTHNPEFTSIE 343
>SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 473
Score = 26.2 bits (55), Expect = 5.4
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +2
Query: 143 WWAARGTGPTLSRSRPQAGPWAAASQV*TPPNYQSLSIWRDRCGMRIFP 289
W G PTLS+ + + WAA SQ+ P + I DRC + P
Sbjct: 130 WLLVTGEIPTLSQVQALSADWAARSQL---PKFVEELI--DRCPPTLHP 173
>SPCC594.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 489
Score = 26.2 bits (55), Expect = 5.4
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +3
Query: 42 NSFIEPAYRVVVDHAQVDLGPDQVPDVVEPVLDHGGPLEGQAPR 173
NS + PA R V A ++ + P+VVEP PL+ AP+
Sbjct: 296 NSDLTPA-RTPVTPAPLEKPVNLAPEVVEPTNAAASPLQLNAPK 338
>SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1038
Score = 25.8 bits (54), Expect = 7.2
Identities = 19/60 (31%), Positives = 25/60 (41%), Gaps = 6/60 (10%)
Frame = -3
Query: 407 ADRGLGLADLITVLDAFFKR--LGFDQL----QFKPAYNPYTEPSMEIFAYHTGLAKWIE 246
AD + LDAF K + FD L YN + + E AY +GL WI+
Sbjct: 874 ADNSETNLNYFATLDAFVKSPLMSFDGLGELGSTDQRYNFFNQKIQEYSAYDSGLDNWIQ 933
>SPAC637.08 |||iron-sulfur cluster assembly ATPase
Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -3
Query: 176 IAWGLSLERPTMIKYGLNNIRDLVGPKV 93
+AWGLSLE I GL ++ D+ GP +
Sbjct: 81 LAWGLSLEEDKQI--GLMDV-DICGPSI 105
>SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit
Nse6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 522
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/40 (27%), Positives = 19/40 (47%)
Frame = +3
Query: 9 FFFYQQSTDLFNSFIEPAYRVVVDHAQVDLGPDQVPDVVE 128
F+ Y+ S++ N + + D +DL QVP +E
Sbjct: 214 FYSYKSSSNNLNQIVRFIFETTADMIGIDLAKRQVPIQLE 253
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,685,741
Number of Sequences: 5004
Number of extensions: 50021
Number of successful extensions: 164
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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