Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_M14
(799 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative ... 259 1e-69
At3g58140.1 68416.m06483 phenylalanyl-tRNA synthetase class IIc ... 55 6e-08
At5g39660.2 68418.m04803 Dof-type zinc finger domain-containing ... 32 0.51
At5g39660.1 68418.m04802 Dof-type zinc finger domain-containing ... 32 0.51
At3g61780.1 68416.m06931 expressed protein ; expression supporte... 31 1.2
At2g18700.1 68415.m02178 glycosyl transferase family 20 protein ... 31 1.2
At4g28780.1 68417.m04115 GDSL-motif lipase/hydrolase family prot... 30 2.0
At3g50370.1 68416.m05508 expressed protein 29 2.7
At1g70290.1 68414.m08087 trehalose-6-phosphate synthase, putativ... 29 2.7
At5g33370.1 68418.m03962 GDSL-motif lipase/hydrolase family prot... 29 3.6
At5g18430.1 68418.m02171 GDSL-motif lipase/hydrolase family prot... 29 3.6
At1g15890.1 68414.m01906 disease resistance protein (CC-NBS-LRR ... 29 3.6
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 29 4.7
At2g28890.1 68415.m03511 protein phosphatase 2C family protein /... 29 4.7
>At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative /
phenylalanine--tRNA ligase, putative similar to
SP|Q9Y285 Phenylalanyl-tRNA synthetase alpha chain (EC
6.1.1.20) (Phenylalanine- -tRNA ligase alpha chain)
(PheRS) {Homo sapiens}; contains Pfam profile PF01409:
tRNA synthetases class II core domain (F)
Length = 485
Score = 259 bits (635), Expect = 1e-69
Identities = 115/154 (74%), Positives = 132/154 (85%)
Frame = -3
Query: 515 QTEFTPQKYFSIDKVFRNETLDATHLAEFHQVEGVVADRGLGLADLITVLDAFFKRLGFD 336
Q F P+KYFSID+VFRNE +D THLAEFHQ+EG++ DRGL L DLI VL+ FF RLG
Sbjct: 330 QKPFVPKKYFSIDRVFRNEAVDRTHLAEFHQIEGLICDRGLTLGDLIGVLEDFFSRLGMS 389
Query: 335 QLQFKPAYNPYTEPSMEIFAYHTGLAKWIEIGNSGVFRPEMLLPMGLPEDVNVIAWGLSL 156
+L+FKPAYNPYTEPSMEIF+YH GL KW+EIGNSG+FRPEMLLPMGLPEDV VIAWGLSL
Sbjct: 390 KLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVIAWGLSL 449
Query: 155 ERPTMIKYGLNNIRDLVGPKVDLRMVYNNPICRL 54
ERPTMI YG++NIRDL G KVDL ++ NPICR+
Sbjct: 450 ERPTMILYGIDNIRDLFGHKVDLDLIKRNPICRI 483
Score = 72.5 bits (170), Expect = 3e-13
Identities = 31/40 (77%), Positives = 34/40 (85%)
Frame = -2
Query: 651 EEVHSKGGYGSQGYRYDWKIEEAQKNLLRTHTTAVSARML 532
+ VH GGYGS+GY YDWK EEA KNLLRTHTTAVS+RML
Sbjct: 286 KRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRML 325
Score = 41.9 bits (94), Expect = 5e-04
Identities = 17/27 (62%), Positives = 18/27 (66%)
Frame = -1
Query: 751 FQPQQXPARDXHDTFFXSSPAATTEFP 671
FQPQQ PARD HDTFF P+ T P
Sbjct: 252 FQPQQHPARDSHDTFFLKVPSTTRTLP 278
>At3g58140.1 68416.m06483 phenylalanyl-tRNA synthetase class IIc
family protein similar to phenylalanine-tRNA synthetase
[Homo sapiens] GI:3983103; contains Pfam profile
PF01409: tRNA synthetases class II core domain (F)
Length = 429
Score = 54.8 bits (126), Expect = 6e-08
Identities = 46/156 (29%), Positives = 72/156 (46%), Gaps = 16/156 (10%)
Frame = -3
Query: 533 CXRLAQQTEFTPQ---KYFSIDKVFRNETLDATHLAEFHQVEGVVA-----------DRG 396
C A Q E + ++ V+R +++D+TH FHQ+EG D
Sbjct: 160 CHTSAHQAELLRKGHSRFLVTGDVYRRDSIDSTHYPVFHQMEGFCVFSPEDWNGSGKDST 219
Query: 395 LGLA-DLITVLDAFFKRL-GFDQLQFKPAYNPYTEPSMEIFAYHTGLAKWIEIGNSGVFR 222
L A DL L+ + L G ++++ Y P+T PS E+ Y W+E+ GV
Sbjct: 220 LYAAEDLKKCLEGLARHLFGSVEMRWVDTYFPFTNPSFELEIYFK--EDWLEVLGCGVTE 277
Query: 221 PEMLLPMGLPEDVNVIAWGLSLERPTMIKYGLNNIR 114
+L GL +V A+GL LER M+ + + +IR
Sbjct: 278 QVILKQSGLENNV-AWAFGLGLERLAMVLFDIPDIR 312
>At5g39660.2 68418.m04803 Dof-type zinc finger domain-containing
protein similar to H-protein promoter binding factor-2a
GI:3386546 from [Arabidopsis thaliana]
Length = 457
Score = 31.9 bits (69), Expect = 0.51
Identities = 22/68 (32%), Positives = 30/68 (44%), Gaps = 4/68 (5%)
Frame = +3
Query: 165 APRYHVHV-LRQAHGQQHLRS---EHPRITNLYPFGETGVVCEYFHAGFGVGVVRRLELQ 332
A Y+ HV + A Q + +HP NL FG V+CE +G + L+ Q
Sbjct: 194 ASHYNRHVSITSAEAMQKVARTDLQHPNGANLLTFGSDSVLCESMASGLNLVEKSLLKTQ 253
Query: 333 LVEAEPLE 356
V EP E
Sbjct: 254 TVLQEPNE 261
>At5g39660.1 68418.m04802 Dof-type zinc finger domain-containing
protein similar to H-protein promoter binding factor-2a
GI:3386546 from [Arabidopsis thaliana]
Length = 457
Score = 31.9 bits (69), Expect = 0.51
Identities = 22/68 (32%), Positives = 30/68 (44%), Gaps = 4/68 (5%)
Frame = +3
Query: 165 APRYHVHV-LRQAHGQQHLRS---EHPRITNLYPFGETGVVCEYFHAGFGVGVVRRLELQ 332
A Y+ HV + A Q + +HP NL FG V+CE +G + L+ Q
Sbjct: 194 ASHYNRHVSITSAEAMQKVARTDLQHPNGANLLTFGSDSVLCESMASGLNLVEKSLLKTQ 253
Query: 333 LVEAEPLE 356
V EP E
Sbjct: 254 TVLQEPNE 261
>At3g61780.1 68416.m06931 expressed protein ; expression supported by
MPSS
Length = 1121
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +3
Query: 294 GFGVGVVRRLELQLVEAEPLEEGVQHGDEVG 386
GFGV V+R+ EL+L EPLEE + E+G
Sbjct: 1041 GFGVTVIRKGELKLNIDEPLEEVEEEICEIG 1071
>At2g18700.1 68415.m02178 glycosyl transferase family 20 protein /
trehalose-phosphatase family protein similar to
trehalose-6-phosphate synthase SL-TPS/P [Selaginella
lepidophylla] GI:4100325; contains Pfam profiles
PF00982: Glycosyltransferase family 20, PF02358:
Trehalose-phosphatase
Length = 862
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +3
Query: 354 EEGVQHGDEVGEPEPAVRDHALHLVELRQVGGVQGLVAEHLI 479
+E + H + V EP V H+VE++ G +G V EHLI
Sbjct: 717 KELLDHLESVLTNEPVVVKRGQHIVEVKPQGVSKGKVVEHLI 758
>At4g28780.1 68417.m04115 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL3 (GI:15054386), EXL1
(GI:15054382), EXL2 (GI:15054384) [Arabidopsis
thaliana]; contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 29.9 bits (64), Expect = 2.0
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 133 YLIMVGRSRDRPHAITFTSSGRPMGSSISGLNTPEL 240
YL+ R+ P+ I + + GRP G +GLN P++
Sbjct: 47 YLVTTARADSPPYGIDYPT-GRPTGRFSNGLNLPDI 81
>At3g50370.1 68416.m05508 expressed protein
Length = 2179
Score = 29.5 bits (63), Expect = 2.7
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 117 PGPGRAQGRPAHGLQQPY 64
PG G++QGRP HG PY
Sbjct: 748 PGWGQSQGRPRHGPYSPY 765
>At1g70290.1 68414.m08087 trehalose-6-phosphate synthase, putative
similar to Alpha,alpha-trehalose-phosphate synthase
[UDP-forming] (EC 2.4.1.15) (Trehalose-6-phosphate
synthase) from {Kluyveromyces lactis} SP|Q07158,
{Saccharomyces cerevisiae} SP|Q00764; contains Pfam
profiles PF00982: Glycosyltransferase family 20,
PF02358: Trehalose-phosphatase
Length = 856
Score = 29.5 bits (63), Expect = 2.7
Identities = 18/51 (35%), Positives = 28/51 (54%)
Frame = +3
Query: 354 EEGVQHGDEVGEPEPAVRDHALHLVELRQVGGVQGLVAEHLIDAEVLLRSE 506
+E + H + V EP V H+VE++ G +GL AE +I E++ R E
Sbjct: 720 KEMLDHLESVLANEPVVVKRGQHIVEVKPQGVSKGLAAEKVI-REMVERGE 769
>At5g33370.1 68418.m03962 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL3 (GI:15054386), EXL1
(GI:15054382), EXL2 (GI:15054384) [Arabidopsis
thaliana]; contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 366
Score = 29.1 bits (62), Expect = 3.6
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +1
Query: 133 YLIMVGRSRDRPHAITFTSSGRPMGSSISGLNTPELPISIHLAR 264
+L R+ + P+ I F + RP G +GLN P+L IS HL +
Sbjct: 46 FLATTARADNYPYGIDFPTH-RPTGRFSNGLNIPDL-ISEHLGQ 87
>At5g18430.1 68418.m02171 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL1 GI:15054382 from
[Arabidopsis thaliana]; contains Pfam profile PF00657:
GDSL-like Lipase/Acylhydrolase
Length = 362
Score = 29.1 bits (62), Expect = 3.6
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 133 YLIMVGRSRDRPHAITFTSSGRPMGSSISGLNTPEL 240
YL+ R+ P+ I F + RP G +GLN P+L
Sbjct: 44 YLVTTARADSPPYGIDFPTR-RPTGRFSNGLNIPDL 78
>At1g15890.1 68414.m01906 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 851
Score = 29.1 bits (62), Expect = 3.6
Identities = 19/60 (31%), Positives = 24/60 (40%)
Frame = -2
Query: 318 GVQPLHRTQHGNIRIPHRSRQMDRDW*FGGVQT*DAAAHGPA*GRERDSVGPVPRAAHHD 139
GV PL R I RS+ + RD G D A + VGP+P +H D
Sbjct: 274 GVPPLTRENGSKIVFTTRSKDVCRDMEVDGEMKVDCLPPDEAWELFQKKVGPIPLQSHED 333
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 28.7 bits (61), Expect = 4.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -3
Query: 497 QKYFSIDKVFRNETLDATHLAEFHQVEGVVA 405
++ + I K FRNE +D TH EF E +A
Sbjct: 349 ERVYEIGKQFRNEGIDLTHNPEFTTCEFYMA 379
>At2g28890.1 68415.m03511 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2c
(GI:3608412) [Mesembryanthemum crystallinum]; contains
Pfam PF00481 : Protein phosphatase 2C domain
Length = 654
Score = 28.7 bits (61), Expect = 4.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 300 GVGVVRRLELQLVEAEPLEEGVQHGDEVGEPEP 398
G G ++ EL ++E +PL+EG+ H P+P
Sbjct: 15 GAGRNKKPELSILEPDPLDEGLGHSFCYVRPDP 47
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,611,111
Number of Sequences: 28952
Number of extensions: 289332
Number of successful extensions: 834
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 834
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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