Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_M03
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 210 1e-55
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 210 1e-55
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 210 1e-55
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 76 5e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 40 5e-04
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.1
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 210 bits (514), Expect = 1e-55
Identities = 96/132 (72%), Positives = 111/132 (84%)
Frame = -2
Query: 720 FQRKERSVKELRRGYVAGDSXNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 541
F K SVK++RRG V GDS N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 540 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 361
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 360 RQTVAVGVIKAV 325
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 210 bits (514), Expect = 1e-55
Identities = 96/132 (72%), Positives = 111/132 (84%)
Frame = -2
Query: 720 FQRKERSVKELRRGYVAGDSXNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 541
F K SVK++RRG V GDS N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 540 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 361
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 360 RQTVAVGVIKAV 325
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 210 bits (514), Expect = 1e-55
Identities = 96/132 (72%), Positives = 111/132 (84%)
Frame = -2
Query: 720 FQRKERSVKELRRGYVAGDSXNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 541
F K SVK++RRG V GDS N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 540 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 361
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 360 RQTVAVGVIKAV 325
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 76.2 bits (179), Expect = 5e-15
Identities = 42/125 (33%), Positives = 68/125 (54%), Gaps = 1/125 (0%)
Frame = -2
Query: 693 ELRRGYVAGDSXNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 514
+++ GYV S NP F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVL-TSTKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 513 VDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-V 337
+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V
Sbjct: 599 LDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657
Query: 336 IKAVN 322
+K ++
Sbjct: 658 VKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 39.5 bits (88), Expect = 5e-04
Identities = 37/129 (28%), Positives = 56/129 (43%)
Frame = -2
Query: 717 QRKERSVKELRRGYVAGDSXNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIAC 538
Q + V +LR G + + NP + F A++ + G I +G T VL H+
Sbjct: 470 QLADIEVNQLRPGDILSN-YENPVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGR 523
Query: 537 KFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMR 358
+ K+ K + + S K I L PLC+ +E P LGRF +R
Sbjct: 524 TVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSG 580
Query: 357 QTVAVGVIK 331
TVA G++K
Sbjct: 581 DTVAAGIVK 589
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.1
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 281 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 454
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 455 SPDLMDFGLTSVDLPVRRSTFSLIS 529
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.4
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 446 MAASPDLMDFGLTSVDLPVRRSTFS 520
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,943,685
Number of Sequences: 5004
Number of extensions: 57211
Number of successful extensions: 161
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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