Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_L23
(849 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 225 5e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 222 4e-59
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 8e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.013
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.5
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.9
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 5.9
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 225 bits (551), Expect = 5e-60
Identities = 100/128 (78%), Positives = 115/128 (89%), Gaps = 1/128 (0%)
Frame = -1
Query: 513 WLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV 337
++A C RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKV 375
Query: 336 QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD 157
RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+D
Sbjct: 376 NRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 435
Query: 156 YEEVGMDS 133
YEEVG DS
Sbjct: 436 YEEVGQDS 443
Score = 110 bits (265), Expect = 2e-25
Identities = 52/98 (53%), Positives = 63/98 (64%), Gaps = 2/98 (2%)
Frame = -3
Query: 781 PTYTNLN--VSSXRFXLXXXFLXSXAXXXGPHRVPDKXVPYPRIHFPLVTYAPVXSAEKA 608
PTY NLN ++ + + + + VPYPRIHFPLVTY+P+ SA KA
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKA 285
Query: 607 YHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 494
+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 286 FHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 222 bits (543), Expect = 4e-59
Identities = 100/131 (76%), Positives = 114/131 (87%), Gaps = 1/131 (0%)
Frame = -1
Query: 522 ASTWLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 346
A ++A C RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 345 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 166
AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL
Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428
Query: 165 EKDYEEVGMDS 133
E+DYEEVG DS
Sbjct: 429 ERDYEEVGQDS 439
Score = 109 bits (263), Expect = 4e-25
Identities = 47/60 (78%), Positives = 51/60 (85%)
Frame = -3
Query: 673 VPYPRIHFPLVTYAPVXSAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 494
VPYPRIHFPLVTYAP+ SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 260 VPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 82.2 bits (194), Expect = 8e-17
Identities = 44/122 (36%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = -1
Query: 492 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 313
RG V K+V+ I +++TK + FV+W P + PP DL + + +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIG 369
Query: 312 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVG 142
N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y+E G
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 141 MD 136
+D
Sbjct: 430 ID 431
Score = 61.7 bits (143), Expect = 1e-10
Identities = 31/98 (31%), Positives = 51/98 (52%), Gaps = 2/98 (2%)
Frame = -3
Query: 781 PTYTNLN--VSSXRFXLXXXFLXSXAXXXGPHRVPDKXVPYPRIHFPLVTYAPVXSAEKA 608
P+Y +LN VS+ + F ++ VP+PR+HF +V +AP+ + +
Sbjct: 220 PSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSS 279
Query: 607 YHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 494
+ +SV E+T F+ N MV DPRHG+Y+ L+
Sbjct: 280 SFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALF 317
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.4 bits (120), Expect = 8e-08
Identities = 34/140 (24%), Positives = 69/140 (49%), Gaps = 4/140 (2%)
Frame = -1
Query: 558 PPTRW*NATPVMASTWLAVC-CTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 382
P + + P S ++++ +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 381 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE- 205
+ P + ++ + ML+N T+IA + R ++D + + AF+ Y E + E
Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFED 416
Query: 204 --GEFSEAREDLAALEKDYE 151
EF +R+ +A L +YE
Sbjct: 417 DLNEFDSSRDVVADLINEYE 436
Score = 30.7 bits (66), Expect = 0.27
Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 3/51 (5%)
Frame = -3
Query: 673 VPYPRIHFPLVTYAPVXS---AEKAYHEQLSVAEITNACFEPANQMVKCDP 530
+P PR HF L +Y P + E + +V ++ P NQMV +P
Sbjct: 261 IPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 35.1 bits (77), Expect = 0.013
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -2
Query: 500 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 357
A PVV P + RP++P P LS V PV+ V + PP P
Sbjct: 549 AAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 29.5 bits (63), Expect = 0.63
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -2
Query: 500 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 357
A PVV P + RP++P P S PV V + PP P
Sbjct: 639 AAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/49 (36%), Positives = 21/49 (42%)
Frame = -2
Query: 497 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 351
VP V S P+ P +P PSV + V P SV P PEA
Sbjct: 643 VPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEA 691
Score = 26.6 bits (56), Expect = 4.4
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = -2
Query: 494 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 354
P V S P+ P +P PSV + V P + SV P PE
Sbjct: 539 PEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPE 585
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -2
Query: 497 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 354
VP S P+ P +P PSV + V P + SV P PE
Sbjct: 628 VPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 675
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -2
Query: 287 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 114
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 113 EPKSTK 96
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.9
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -2
Query: 455 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 369
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 5.9
Identities = 26/101 (25%), Positives = 40/101 (39%)
Frame = -2
Query: 494 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWPRFNVXXXXX 315
PVV + P T+P S P+ S + PVS + T + PP A R N
Sbjct: 988 PVVQNKPAA-TKPV--SMPAAKSKPAPMANPVSTAQQTQNRPP--APAMQARPNTTQAAA 1042
Query: 314 XXXXXXPKLGLALTTSSTSCTPSVLSCTGTSVRVWRRESSP 192
+ A T S++ PS ++ +S + S+P
Sbjct: 1043 PVTSTTTTIKQATTVSASKPAPSTVTSAASSPSNISKPSAP 1083
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,044,987
Number of Sequences: 5004
Number of extensions: 64277
Number of successful extensions: 238
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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