Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_L10
(845 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25530.1 68418.m03038 DNAJ heat shock protein, putative simla... 50 2e-06
At5g01390.1 68418.m00052 DNAJ heat shock protein, putative simil... 46 2e-05
At3g08910.1 68416.m01037 DNAJ heat shock protein, putative simil... 46 2e-05
At2g20560.1 68415.m02401 DNAJ heat shock family protein SP|Q9UDY... 42 4e-04
At2g20550.1 68415.m02400 DNAJ chaperone C-terminal domain-contai... 41 9e-04
At3g62600.1 68416.m07032 DNAJ heat shock family protein similar ... 40 0.002
At4g28480.1 68417.m04074 DNAJ heat shock family protein contains... 40 0.002
At1g59725.1 68414.m06724 DNAJ heat shock protein, putative simil... 39 0.004
At3g47940.1 68416.m05227 DNAJ heat shock protein, putative simil... 38 0.006
At1g10350.1 68414.m01166 DNAJ heat shock protein, putative simil... 38 0.006
At1g11040.1 68414.m01265 DNAJ chaperone C-terminal domain-contai... 36 0.034
At5g22060.1 68418.m02569 DNAJ heat shock protein, putative stron... 35 0.059
At3g44110.1 68416.m04727 DNAJ heat shock protein, putative (J3) ... 35 0.078
At1g44160.1 68414.m05100 DNAJ chaperone C-terminal domain-contai... 34 0.14
At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein le... 30 2.2
>At5g25530.1 68418.m03038 DNAJ heat shock protein, putative simlar
to SP|P25685 DnaJ homolog subfamily B member 1 (Heat
shock 40 kDa protein 1) {Homo sapiens}; contains Pfam
profile PF00226: DnaJ domain
Length = 347
Score = 50.0 bits (114), Expect = 2e-06
Identities = 22/41 (53%), Positives = 26/41 (63%)
Frame = -3
Query: 549 PGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTL 427
PG G+P KEP KGDL + FD++FP RLTT K L L
Sbjct: 305 PGEGMPIAKEPRNKGDLKIKFDVQFPARLTTEQKSALKRVL 345
>At5g01390.1 68418.m00052 DNAJ heat shock protein, putative similar
to SP|Q9QYJ3 DnaJ homolog subfamily B member 1 (Heat
shock 40 kDa protein 1) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 335
Score = 46.4 bits (105), Expect = 2e-05
Identities = 21/40 (52%), Positives = 29/40 (72%), Gaps = 4/40 (10%)
Frame = -3
Query: 546 GYGLPFPKEPTRKGDLLVAFDIKFPERLTT----GVKEIL 439
G G+P PK+P+RKG+L + F IKFP +LTT G+K +L
Sbjct: 294 GEGMPIPKDPSRKGNLRIRFIIKFPSKLTTEQKSGIKRML 333
>At3g08910.1 68416.m01037 DNAJ heat shock protein, putative similar
to SP|P25685 DnaJ homolog subfamily B member 1 (Heat
shock 40 kDa protein 1) {Homo sapiens}; contains Pfam
profile PF00226: DnaJ domain
Length = 323
Score = 46.4 bits (105), Expect = 2e-05
Identities = 18/33 (54%), Positives = 25/33 (75%)
Frame = -3
Query: 546 GYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVK 448
G G+P PK+P++KG+L + F +KFP RLTT K
Sbjct: 281 GEGMPIPKDPSKKGNLRIKFTVKFPSRLTTEQK 313
>At2g20560.1 68415.m02401 DNAJ heat shock family protein SP|Q9UDY4
DnaJ homolog subfamily B member 4 (Heat shock 40 kDa
protein 1 homolog) {Homo sapiens}; contains Pfam profile
PF00226: DnaJ domain
Length = 337
Score = 42.3 bits (95), Expect = 4e-04
Identities = 20/41 (48%), Positives = 29/41 (70%), Gaps = 4/41 (9%)
Frame = -3
Query: 549 PGYGLPFPKEPTRKGDLLVAFDIKFPERLT----TGVKEIL 439
P G+P K+ T++G+L + F+IKFP RLT TGVK++L
Sbjct: 296 PKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKLL 336
>At2g20550.1 68415.m02400 DNAJ chaperone C-terminal
domain-containing protein contains Pfam profile PF01556:
DnaJ C terminal region; similar to DnaJ-like proteins
(GI:6179940) [Nicotiana tabacum] and(GI:11863723)
[Lycopersicon esculentum]; similar to DnaJ homolog
subfamily B member 1 (Heat shock 40 kDa protein 1) (Heat
shock protein 40) (HSP40) (DnaJ protein homolog 1)
(HDJ-1) (Swiss-Prot:P25685) [Homo sapiens] and
(Swiss-Prot:Q9QYJ3) [Mus musculus]
Length = 284
Score = 41.1 bits (92), Expect = 9e-04
Identities = 20/48 (41%), Positives = 30/48 (62%), Gaps = 4/48 (8%)
Frame = -3
Query: 570 PHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLT----TGVKEIL 439
P V+ P G+P K+ +KG+L + F+IKFP LT TG+K++L
Sbjct: 236 PEYVEVVPNEGMPLQKDQAKKGNLRIKFNIKFPTTLTSEQKTGLKKLL 283
>At3g62600.1 68416.m07032 DNAJ heat shock family protein similar to
DnaJ homolog subfamily B member 11 precursor
(SP:Q99KV1){Mus musculus}; contains Pfam PF00226: DnaJ
domain; contains PfaPF01556: DnaJ C terminal regionm
Length = 346
Score = 40.3 bits (90), Expect = 0.002
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = -3
Query: 576 SKPHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKE 445
+KP VK+F G G+P T+KG+L V F++ FP LT K+
Sbjct: 297 TKPKEVKKFKGEGMPLHYS-TKKGNLFVTFEVLFPSSLTDDQKK 339
>At4g28480.1 68417.m04074 DNAJ heat shock family protein contains
Pfam profile PF00226: DnaJ domain; ; similar to DnaJ
homolog subfamily B member 1 (Heat shock 40 kDa protein
1) (Heat shock protein 40) (HSP40) (DnaJ protein homolog
1) (HDJ-1) (Swiss-Prot:P25685) [Homo sapiens] and
(Swiss-Prot:Q9QYJ3) [Mus musculus]
Length = 348
Score = 39.9 bits (89), Expect = 0.002
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = -3
Query: 549 PGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVK 448
P G+P K+ T+KG+L + F+IKFP RLT K
Sbjct: 307 PKEGMPLQKDQTKKGNLRIKFNIKFPARLTAEQK 340
>At1g59725.1 68414.m06724 DNAJ heat shock protein, putative similar
to SP|Q9QYJ3 DnaJ homolog subfamily B member 1 (Heat
shock 40 kDa protein 1) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 331
Score = 39.1 bits (87), Expect = 0.004
Identities = 20/49 (40%), Positives = 27/49 (55%)
Frame = -3
Query: 573 KPHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTL 427
KP P G+P KE +++GDL + F+I FP RLT+ K L L
Sbjct: 280 KPGQEIVIPSEGMPISKEGSKRGDLRINFEICFPSRLTSEQKTDLKRVL 328
>At3g47940.1 68416.m05227 DNAJ heat shock protein, putative similar
to SP|O89114 DnaJ homolog subfamily B member 5 (Heat
shock protein Hsp40-3) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 350
Score = 38.3 bits (85), Expect = 0.006
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = -3
Query: 549 PGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVK 448
P G+P KEP +KG+L + +K+P RLT+ K
Sbjct: 306 PNEGMPISKEPGKKGNLKLKLSVKYPSRLTSDQK 339
>At1g10350.1 68414.m01166 DNAJ heat shock protein, putative similar
to SP|Q9QYJ3 DnaJ homolog subfamily B member 1 (Heat
shock 40 kDa protein 1) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 349
Score = 38.3 bits (85), Expect = 0.006
Identities = 21/49 (42%), Positives = 27/49 (55%)
Frame = -3
Query: 573 KPHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTL 427
KP P G+P K+P ++GDL V F+I FP RLT+ K L L
Sbjct: 299 KPGQEIVIPNEGMP-TKDPLKRGDLRVTFEILFPSRLTSEQKNDLKRVL 346
>At1g11040.1 68414.m01265 DNAJ chaperone C-terminal
domain-containing protein contains Pfam profile PF01556:
DnaJ C terminal region
Length = 438
Score = 35.9 bits (79), Expect = 0.034
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 558 KRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTL 427
K G G+P KE ++GDL + F + FPE+L+ + + + L
Sbjct: 389 KAIKGQGMPNAKEEGKRGDLRITFLVNFPEKLSEEQRSMAYEVL 432
>At5g22060.1 68418.m02569 DNAJ heat shock protein, putative strong
similarity to SP|O60884 DnaJ homolog subfamily A member
2 (Dnj3) Homo sapiens, several plant DnaJ proteins from
PGR; contains Pfam profiles PF00226 DnaJ domain, PF00684
DnaJ central domain (4 repeats), PF01556 DnaJ C terminal
region
Length = 419
Score = 35.1 bits (77), Expect = 0.059
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -3
Query: 573 KPHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTLP 424
KP + K G+P + P KG L + F ++FPE L+ + + LP
Sbjct: 311 KPDSYKAISDEGMPIYQRPFMKGKLYIHFTVEFPESLSPDQTKAIEAVLP 360
>At3g44110.1 68416.m04727 DNAJ heat shock protein, putative (J3)
identical to AtJ3 [Arabidopsis thaliana] GI:2641638,
strong similarity to several plant DnaJ proteins from
PGR; contains Pfam profiles PF00226 DnaJ domain, PF00684
DnaJ central domain (4 repeats), PF01556 DnaJ C terminal
region
Length = 420
Score = 34.7 bits (76), Expect = 0.078
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -3
Query: 573 KPHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLTTGVKEILMDTLP 424
KP + K G+P + P KG L + F ++FP+ L+ + L LP
Sbjct: 310 KPDSYKAISDEGMPIYQRPFMKGKLYIHFTVEFPDSLSPDQTKALEAVLP 359
>At1g44160.1 68414.m05100 DNAJ chaperone C-terminal
domain-containing protein contains Pfam profile PF01556:
DnaJ C terminal region
Length = 357
Score = 33.9 bits (74), Expect = 0.14
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = -3
Query: 570 PHTVKRFPGYGLPFPKEPTRKGDLLVAFDIKFPERLT 460
P V G G+P KE ++GDL V F KFP+ LT
Sbjct: 306 PGYVTVVQGKGMPNLKEKGKRGDLRVRFRTKFPQHLT 342
>At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein
leucine zipper-containing protein - Lycopersicon
esculentum, EMBL:Z12127 contains Pfam domain PF03081:
Exo70 exocyst complex subunit;
Length = 653
Score = 29.9 bits (64), Expect = 2.2
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +3
Query: 102 VVLERTKNNVINKLY*SIHSLQNNWQQHDTQCSRSPKMSSLSLGLSI*RFWFAKTQETLP 281
V+ ER K + N ++ IH Q+ W D Q ++S SL + R +F + ++ L
Sbjct: 561 VLKERFK--IFNAMFDEIHKTQSTWIVSDEQMQSELRVSISSLVIPAYRSFFGRYKQHLD 618
Query: 282 PFDQANTY 305
Q + Y
Sbjct: 619 SGKQTDKY 626
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,515,155
Number of Sequences: 28952
Number of extensions: 300447
Number of successful extensions: 593
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 592
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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