Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_T7_K10
         (827 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str...    71   9e-13
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S...    66   3e-11
At1g69060.1 68414.m07902 expressed protein                             29   3.8  
At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pf...    28   8.7  
At3g58160.1 68416.m06485 myosin heavy chain, putative similar to...    28   8.7  
At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box fami...    28   8.7  
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-...    28   8.7  

>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
           similarity to 60S ribosomal protein L1 GB:P49691
          Length = 406

 Score = 70.9 bits (166), Expect = 9e-13
 Identities = 37/94 (39%), Positives = 55/94 (58%)
 Frame = -3

Query: 582 TQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRA 403
           T+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ SDEI+ V+    K   RA
Sbjct: 259 TKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEIQSVVNPIKKDAKRA 318

Query: 402 TRKLNPLTNNQGDAETQSLRGRAEEESYLRAAQK 301
             K NPL N     +       A+  S L  AQ+
Sbjct: 319 VLKKNPLKNLNVMLKLNPYAKTAKRMSLLAEAQR 352



 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 43/138 (31%), Positives = 61/138 (44%), Gaps = 2/138 (1%)
 Frame = -1

Query: 632 DLLKLAPGGHFGRFVI*LSPHSAGLTPYSGHGRHHQNKRRTSTCPNRRW--PTLTSHVFS 459
           +LLKLAPGGH GRFVI        L    G       K++    P  +     L   + S
Sbjct: 242 NLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINS 301

Query: 458 SLMRSGRSSVLPTNA*SVLHAN*TRSLTTKAMLKLNPYAAVLKRKAILELRRRKNLKALA 279
             ++S  + +      +VL  N  ++L    MLKLNPYA   KR ++L   +R   K   
Sbjct: 302 DEIQSVVNPIKKDAKRAVLKKNPLKNL--NVMLKLNPYAKTAKRMSLLAEAQRVKAKKEK 359

Query: 278 DAEKSGLKLSKRNPAMKA 225
            A+K      +   A+KA
Sbjct: 360 LAKKRKTVTKEEALAIKA 377


>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
           roibosomal protein L4, Arabidopsis thaliana,
           EMBL:CAA79104
          Length = 407

 Score = 66.1 bits (154), Expect = 3e-11
 Identities = 30/69 (43%), Positives = 46/69 (66%)
 Frame = -3

Query: 582 TQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRA 403
           T+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ SDE++ V+        RA
Sbjct: 260 TKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEVQSVVNPIKDGSKRA 319

Query: 402 TRKLNPLTN 376
             K NPL N
Sbjct: 320 VLKKNPLKN 328



 Score = 47.6 bits (108), Expect = 1e-05
 Identities = 41/138 (29%), Positives = 60/138 (43%), Gaps = 2/138 (1%)
 Frame = -1

Query: 632 DLLKLAPGGHFGRFVI*LSPHSAGLTPYSGHGRHHQNKRRTSTCPNRRW--PTLTSHVFS 459
           +LLKLAPGGH GRFVI        L    G       K++    P  +     L   + S
Sbjct: 243 NLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINS 302

Query: 458 SLMRSGRSSVLPTNA*SVLHAN*TRSLTTKAMLKLNPYAAVLKRKAILELRRRKNLKALA 279
             ++S  + +   +  +VL  N  ++L    M KLNPYA   KR ++L    R   K   
Sbjct: 303 DEVQSVVNPIKDGSKRAVLKKNPLKNL--NVMFKLNPYAKTAKRMSLLAEASRVKAKKEK 360

Query: 278 DAEKSGLKLSKRNPAMKA 225
             +K  +   +   A+KA
Sbjct: 361 LEKKRKVVTKEEAQAIKA 378


>At1g69060.1 68414.m07902 expressed protein
          Length = 630

 Score = 29.1 bits (62), Expect = 3.8
 Identities = 21/90 (23%), Positives = 34/90 (37%), Gaps = 1/90 (1%)
 Frame = -3

Query: 591 RHLTQSAFGRLDPLFG-SWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKR 415
           +H   S F   +   G S  +P  + ++     P    T     +     +KV+  PNK 
Sbjct: 307 KHTANSTFSSKEDFRGPSPLSPGIRVEHERFDTPPTHFTSPKEPIHQTSFKKVVEQPNKV 366

Query: 414 VIRATRKLNPLTNNQGDAETQSLRGRAEEE 325
            ++    L+P  N   +   QS     EEE
Sbjct: 367 QVQCETSLSPEENQNKNVAYQSYTKEVEEE 396


>At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 654

 Score = 27.9 bits (59), Expect = 8.7
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = -2

Query: 604 ILDVSSSDSVRIRQA*PLIRVMEDTIKTKEELQPAPTEDGQH 479
           +L VS+   VRI Q   ++R  +   KT+     + T DG+H
Sbjct: 476 VLVVSADSKVRILQGNNVVRKYKGVCKTRSLTSASLTSDGKH 517


>At3g58160.1 68416.m06485 myosin heavy chain, putative similar to
            myosin heavy chain [Arabidopsis thaliana]
            gi|602328|emb|CAA84067.
          Length = 1242

 Score = 27.9 bits (59), Expect = 8.7
 Identities = 16/34 (47%), Positives = 21/34 (61%)
 Frame = -1

Query: 293  LKALADAEKSGLKLSKRNPAMKAEKLRERRRKNI 192
            LKAL   EK    +S+R  A +A +L ERRRK +
Sbjct: 1092 LKALLQVEKQRADISERKCA-EARELGERRRKRL 1124


>At1g30950.1 68414.m03790 unusual floral organ (UFO) / F-box family
           protein (FBX1) E3 ubiquitin ligase SCF complex F-box
           subunit; almost identical to unusual floral organs
           (UFO)GI:4376159 from [Arabidopsis thaliana]
           Landsberg-erecta; one amino acid difference
          Length = 442

 Score = 27.9 bits (59), Expect = 8.7
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +2

Query: 92  RPFQLSASYLPSSWEEAGSS*QPASWVS 175
           R ++LS +Y+PS +  +GSS    SWVS
Sbjct: 138 RWYRLSFAYIPSGFYPSGSSGGLVSWVS 165


>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
           domain-containing protein weak similarity to SP|P45974
           Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
           {Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
           domain
          Length = 413

 Score = 27.9 bits (59), Expect = 8.7
 Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
 Frame = -1

Query: 338 VLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLR-ERRRK 198
           +L+ K + E+  RK L  L  AEK   K ++     K E+ + ERRRK
Sbjct: 218 LLEAKRMEEVNERKRLMFLRKAEKEEEKRAREKIRQKLEEDKAERRRK 265


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,653,179
Number of Sequences: 28952
Number of extensions: 239029
Number of successful extensions: 640
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 638
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)