Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_K08
(796 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 136 3e-33
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 136 3e-33
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 136 3e-33
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 46 8e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.11
SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein L17|Sch... 28 1.8
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 26 5.4
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 9.4
SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein L17|Sc... 25 9.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 136 bits (330), Expect = 3e-33
Identities = 66/108 (61%), Positives = 75/108 (69%)
Frame = -3
Query: 791 ITTEVKSVGMHXXSSPRSCTWRQCRFQRKERVRQGLRRGYVAGDSKNNPPKGAADFTAQV 612
+TTEVKSV MH S F K + +RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 611 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSN*SQPK 468
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK PK
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Score = 90.6 bits (215), Expect = 2e-19
Identities = 42/61 (68%), Positives = 48/61 (78%)
Frame = -1
Query: 472 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 293
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 292 V 290
V
Sbjct: 449 V 449
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 136 bits (330), Expect = 3e-33
Identities = 66/108 (61%), Positives = 75/108 (69%)
Frame = -3
Query: 791 ITTEVKSVGMHXXSSPRSCTWRQCRFQRKERVRQGLRRGYVAGDSKNNPPKGAADFTAQV 612
+TTEVKSV MH S F K + +RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 611 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSN*SQPK 468
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK PK
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Score = 90.6 bits (215), Expect = 2e-19
Identities = 42/61 (68%), Positives = 48/61 (78%)
Frame = -1
Query: 472 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 293
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 292 V 290
V
Sbjct: 449 V 449
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 136 bits (330), Expect = 3e-33
Identities = 66/108 (61%), Positives = 75/108 (69%)
Frame = -3
Query: 791 ITTEVKSVGMHXXSSPRSCTWRQCRFQRKERVRQGLRRGYVAGDSKNNPPKGAADFTAQV 612
+TTEVKSV MH S F K + +RRG V GDSKN+PP G A FTAQV
Sbjct: 283 VTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQV 342
Query: 611 IVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSN*SQPK 468
I+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK PK
Sbjct: 343 IILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPK 390
Score = 90.6 bits (215), Expect = 2e-19
Identities = 42/61 (68%), Positives = 48/61 (78%)
Frame = -1
Query: 472 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 293
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 292 V 290
V
Sbjct: 449 V 449
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 45.6 bits (103), Expect = 8e-06
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 3/82 (3%)
Frame = -3
Query: 722 CRFQRKERVR---QGLRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCH 552
C Q + RVR ++ GYV +KN P F AQ+ +L P ++ GY+ V+ H
Sbjct: 526 CGDQVRLRVRGDDSDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIH 584
Query: 551 TAHIACKFAEIKEKVDRRTGKS 486
TA FA++ K+D+ KS
Sbjct: 585 TAVEEVSFAKLLHKLDKTNRKS 606
Score = 40.7 bits (91), Expect = 2e-04
Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = -1
Query: 472 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 317
P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.11
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -1
Query: 418 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 326
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 3|||Manual
Length = 187
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +3
Query: 465 DFGLTSVRFTSTTVNFFFDFCKFA 536
+FG+T R+ +VNFF+D K A
Sbjct: 75 EFGVTQARWPVKSVNFFYDLLKNA 98
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 288 LTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 410
L L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 25.4 bits (53), Expect = 9.4
Identities = 19/81 (23%), Positives = 36/81 (44%), Gaps = 8/81 (9%)
Frame = -3
Query: 650 NPPKGAADFTAQVIVLNHPGQISNGY--------TPVLDCHTAHIACKFAEIKEKVDRRT 495
NP K + + +A ++ N P Q++ T +L+ ++H + ++EK + +
Sbjct: 1092 NPKKQSGNTSADQVLFNQPKQVTEKLGVQLNLPKTKILEKVSSHSLAQITALREK-EPKD 1150
Query: 494 GKSN*SQPKIHQVWRCSHCQL 432
S S+ KI H QL
Sbjct: 1151 ADSTYSKEKIFSATSTVHLQL 1171
>SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 465 DFGLTSVRFTSTTVNFFFDFCKFA 536
+FG+T R+ +V FF+D K A
Sbjct: 75 EFGVTQARWPVKSVKFFYDLLKNA 98
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,064,540
Number of Sequences: 5004
Number of extensions: 60659
Number of successful extensions: 160
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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